App (NM_019288) Rat Tagged ORF Clone

CAT#: RR204173

App (Myc-DDK-tagged ORF) - Rat amyloid beta (A4) precursor protein (App), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: App
• Synonyms: Abeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204173 representing NM_019288
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCAGCCTGGCACTGCTCCTGCTGGCCGCCTGGACGGTTCGGGCTCTGGAGGTGCCCACTGATG
  GCAATGCCGGTCTGCTGGCAGAACCCCAGATCGCCATGTTCTGTGGTAAACTCAACATGCACATGAATGT
  GCAGAATGGAAAATGGGAGTCAGACCCATCAGGGACCAAAACCTGCATTGGCACCAAGGAGGGAATCCTG
  CAGTACTGCCAAGAGGTCTACCCTGAACTGCAGATCACAAACGTGGTGGAAGCCAACCAGCCAGTGACCA
  TCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACGCACACCCACATCGTGATTCCTTACCGGTG
  CCTAGTTGGTGAGTTTGTAAGCGATGCCCTTCTCGTGCCCGACAAGTGCAAGTTTCTACACCAGGAGCGG
  ATGGACGTTTGTGAGACCCATCTTCACTGGCATACTGTTGCCAAAGAGACATGCAGTGAGAAGAGCACTA
  ACTTGCACGACTATGGCATGCTGCTGCCCTGTGGCATCGACAAGTTCCGAGGGGTCGAGTTCGTGTGCTG
  CCCGTTGGCGGAGGAGAGCGACAGCATCGATTCTGCGGACGCAGAGGAGGACGACTCCGATGTCTGGTGG
  GGTGGAGCGGACACAGACTATGCTGATGGCGGTGAAGACAAAGTCGTAGAAGTAGCCGAAGAGGAGGAAG
  TGGCCGATGTTGAGGAAGAAGAAGCTGAGGATGACGAGGATGTGGAGGATGGGGATGAGGTGGAGGAGGA
  GGCCGAGGAGCCCTACGAAGAGGCCACAGAGAGAACAACCAGCATTGCCACCACTACCACAACTACCACT
  GAGTCTGTGGAGGAGGTAGTCCGAGAGGTGTGCTCTGAACAAGCTGAGACCGGGCCATGCCGTGCAATGA
  TCTCCCGCTGGTACTTTGATGTCACTGAAGGAAAGTGCGCCCCATTCTTTTACGGCGGATGTGGCGGCAA
  CAGGAACAACTTTGACACTGAAGAGTACTGCATGGCGGTGTGTGGCAGCGTGTCATCCCAAAGTTTACTC
  AAGACTACCAGTGAACCTCTTCCCCAAGATCCGGTTAAACTTCCCACGACGGCAGCCAGCACCCCTGACG
  CAGTCGACAAGTACCTGGAGACCCCCGGAGATGAGAACGAGCACGCCCATTTCCAGAAAGCCAAAGAGAG
  GTTGGAAGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAGGAGGCAGAACGTCAAGCC
  AAGAATTTGCCCAAAGCTGACAAGAAGGCCGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTCTGGAAC
  AGGAAGCAGCCAACGAGAGGCAGCAGCTTGTAGAGACACACATGGCCAGAGTTGAAGCCATGCTCAATGA
  TCGCCGTCGCCTGGCCCTCGAGAATTACATCACCGCACTGCAGGCGGTGCCTCCAAGGCCTCATCATGTG
  TTCAACATGCTGAAGAAGTACGTCCGTGCAGAGCAGAAGGACAGACAGCACACCCTAAAGCATTTTGAAC
  ATGTGCGCATGGTGGACCCCAAGAAAGCTGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGAT
  CTACGAGCGCATGAACCAGTCTCTGTCCCTGCTCTACAACGTCCCTGCCGTGGCTGAGGAGATTCAAGAT
  GAAGTTGACGAGCTGCTTCAGAAGGAGCAGAACTACTCCGACGACGTCTTAGCCAACATGATCAGTGAAC
  CCAGAATCAGTTACGGCAACGATGCTCTCATGCCTTCTTTGACTGAAACGAAGACCACTGTGGAGCTCCT
  TCCCGTGAATGGCGAATTCAGCCTGGATGATCTCCAACCGTGGCATCCTTTTGGGGTGGACTCTGTGCCA
  GCCAATACAGAAAATGAAGTTGAGCCTGTCGACGCCCGCCCCGCTGCTGACCGAGGACTGACCACTCGAC
  CAGGGTCTGGGTTGACAAACATCAAGACAGAAGAGATCTCAGAAGTGAAGATGGATGCGGAGTTCGGACA
  TGATTCAGGCTTCGAAGTCCGCCATCAAAAACTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGT
  GCCATCATTGGACTCATGGTGGGTGGCGTTGTCATAGCAACAGTGATTGTCATCACCTTGGTGATGCTGA
  AGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAGGTTGACGCTGCTGTGACCCCGGAGGAGCG
  CCACCTCTCCAAGATGCAGCAGAATGGATATGAGAATCCAACATACAAGTTCTTTGAGCAGATGCAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204173 representing NM_019288
  Red=Cloning site Green=Tags(s)
  
  MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGIL
  QYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQER
  MDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSIDSADAEEDDSDVWW
  GGADTDYADGGEDKVVEVAEEEEVADVEEEEAEDDEDVEDGDEVEEEAEEPYEEATERTTSIATTTTTTT
  ESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSVSSQSLL
  KTTSEPLPQDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQA
  KNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHV
  FNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQD
  EVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVP
  ANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKG
  AIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019288
• ORF Size: 2310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019288.1, NM_019288.2, NP_062161.1
• RefSeq Size: 2340
• RefSeq ORF: 2313
• Locus ID: 54226
• MW: 86.7 kDa
• Gene Summary: activates voltage dependent calcium channels; may induce neuronal apoptosis [RGD, Feb 2006]