Serpinc1 (NM_001012027) Rat Tagged ORF Clone

CAT#: RR204339

Serpinc1 (Myc-DDK-tagged ORF) - Rat serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 (Serpinc1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Serpinc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204339 representing NM_001012027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCCCCGGGAATAGGAAGTGCGGTTGCTGGAGAGAGGAAGCTTTGTCTCCTCTCTCTGCTACTCA
  TTGGTGCCTTGGGCTGTGCTGTCTGTCATGGAAACCCTGTGGACGACATCTGCATAGCGAAGCCCCGAGA
  CATCCCCGTGAACCCCATGTGCATTTACCGCTCCCCTGCGAAGAAGGCCACGGAGGAGGATGTCCTAGAG
  CAGAAGGTTCCGGAAGCCACCAACCGGCGGGTCTGGGAACTGTCCAAGGCCAATTCTCGATTTGCCACTA
  ACTTCTATCAGCACCTGGCAGACTCCAAGAACGACAACGACAACATTTTCCTGTCACCCTTGAGCATCTC
  CACGGCGTTTGCTATGACCAAGCTGGGTGCTTGTAATAACACCCTCAAGCAGCTGATGGAGGTTTTTAAA
  TTTGATACCATCTCCGAGAAGACATCCGACCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCT
  ATCGAAAAGCCAACAAGTCCTCTAACTTGGTGTCAGCCAACCGCCTTTTTGGAGACAAATCCCTTACCTT
  CAATGAGAGCTATCAAGACGTTAGTGAGATTGTCTATGGAGCCAAGCTTCAGCCCCTGGACTTCAAGGAG
  AATCCGGAGCAATCCAGAGTGACCATCAACAACTGGGTAGCTAATAAGACTGAAGGCCGCATCAAAGACG
  TCATCCCCCAAGGAGCCATTGATGAGCTCACTGCCCTGGTGCTGGTTAACACCATTTACTTCAAGGGCCT
  GTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACCATTCCACAAAGTTGATGGGCAGTCATGC
  CTGGTGCCCATGATGTACCAGGAAGGCAAATTCAAATACAGGCGTGTGGGAGAGGGTACCCAGGTGCTAG
  AGATGCCCTTCAAGGGGGACGACATCACCATGGTGCTCATCCTGCCCAAGCCTGAGAAGAGCCTGGCTAA
  GGTGGAGCAGGAACTCACCCCGGAGCTGCTGCAGGAGTGGCTGGATGAGCTGTCGGAGGTCATGCTTGTG
  GTCCACGTGCCCCGCTTCCGCATCGAGGACAGCTTCAGTCTGAAGGAGCAGCTGCAAGACATGGGCCTTG
  TTGATCTCTTCAGCCCTGAGAAGTCCCAACTCCCAGGGATCATTGCTGAAGGCAGGGACGACCTCTTTGT
  CTCCGATGCATTCCACAAAGCGTTTCTTGAGGTAAATGAGGAAGGCAGTGAAGCAGCAGCGAGTACTTCT
  GTCGTGATTACTGGCCGGTCACTGAACCCCAGTAGGGTGACCTTCAAGGCCAACAGGCCCTTCCTGGTTC
  TTATAAGGGAAGTCGCACTGAACACTATTATATTCATGGGGAGAGTGTCTAATCCTTGTGTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204339 representing NM_001012027
  Red=Cloning site Green=Tags(s)
  
  MYSPGIGSAVAGERKLCLLSLLLIGALGCAVCHGNPVDDICIAKPRDIPVNPMCIYRSPAKKATEEDVLE
  QKVPEATNRRVWELSKANSRFATNFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNNTLKQLMEVFK
  FDTISEKTSDQIHFFFAKLNCRLYRKANKSSNLVSANRLFGDKSLTFNESYQDVSEIVYGAKLQPLDFKE
  NPEQSRVTINNWVANKTEGRIKDVIPQGAIDELTALVLVNTIYFKGLWKSKFSPENTRKEPFHKVDGQSC
  LVPMMYQEGKFKYRRVGEGTQVLEMPFKGDDITMVLILPKPEKSLAKVEQELTPELLQEWLDELSEVMLV
  VHVPRFRIEDSFSLKEQLQDMGLVDLFSPEKSQLPGIIAEGRDDLFVSDAFHKAFLEVNEEGSEAAASTS
  VVITGRSLNPSRVTFKANRPFLVLIREVALNTIIFMGRVSNPCVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012027
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012027.1, NP_001012027.1
• RefSeq Size: 1561 bp
• RefSeq ORF: 1398 bp
• Locus ID: 304917
• Cytogenetics: 13q22
• MW: 52.2 kDa