Cyp17a1 (NM_012753) Rat Tagged ORF Clone

CAT#: RR204575

Cyp17a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 17, subfamily a, polypeptide 1 (Cyp17a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp17a1
• Synonyms: Cyp17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204575 representing NM_012753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGAACTTGTGGGTCTCTTGCTGCTCATCCTAGCCTATTTCTTTTGGGTCAAGTCAAAGACGCCCG
  GTGCCAAGCTCCCCAGGAGCCTTCCATCCCTGCCCCTGGTGGGCAGTCTGCCGTTTCTCCCCAGACGTGG
  TCATATGCATGTCAACTTCTTCAAGCTACAGGAAAAGTATGGTCCCATCTATTCTCTTCGCCTGGGTACC
  ACAACTACAGTGATCATCGGCCACTATCAGCTGGCCAGGGAGGTGCTCATCAAGAAGGGAAAAGAATTCT
  CTGGTCGGCCCCAGATGGTGACTCAAAGCCTCTTGTCGGACCAAGGGAAAGGCGTGGCCTTTGCAGATGC
  TGGTAGCTCCTGGCACCTGCACCGGAAGTTGGTGTTCAGCACCTTTTCCCTGTTCAAGGATGGCCAGAAA
  CTGGAGAAGCTAATCTGTCAGGAAGCCAAGTCACTGTGTGATATGATGCTGGCACACGACAAGGAGTCCA
  TAGACCTGTCCACGCCTATCTTCATGTCAGTAACCAACATCATCTGTGCTATCTGCTTCAACATCTCTTA
  TGAGAAAAATGATCCAAAACTGACCGCCATAAAGACCTTTACTGAGGGTATCGTGGATGCCACGGGCGAC
  AGAAATCTGGTGGACATATTCCCATGGTTGACGATTTTTCCCAACAAAGGCTTGGAGGTGATAAAGGGTT
  ATGCCAAAGTTCGAAATGAAGTACTGACTGGAATATTTGAAAAGTGCAGGGAGAAGTTCGACAGTCAGTC
  TATCTCCAGCCTGACGGACATTCTGATTCAAGCCAAGATGAATTCAGACAACAACAACAGCTGTGAAGGC
  CGGGACCCAGATGTGTTTTCAGATAGGCACATCCTTGCCACGGTGGGAGACATCTTTGGGGCGGGCATAG
  AGACAACTACCACTGTGCTCAAGTGGATCCTGGCTTTCCTGGTGCACAATCCTGAGGTGAAGAAGAAGAT
  CCAAAAGGAGATTGACCAGTACGTAGGCTTCAGCCGAACACCAACTTTCAATGACCGGTCTCACCTCCTC
  ATGCTGGAGGCCACTATCCGAGAAGTGCTGCGTATCAGGCCGGTGGCTCCCATGCTCATCCCCCACAAGG
  CTAACGTTGACTCCAGCATTGGAGAGTTTACTGTCCCCAAGGACACACATGTGGTCGTCAATCTCTGGGC
  ACTGCATCACGATGAGAATGAATGGGACCAGCCAGATCAGTTCATGCCTGAACGCTTCTTAGATCCAACG
  GGAAGCCATCTCATTACACCCACGCAGAGTTACTTGCCCTTCGGAGCTGGTCCCCGATCCTGCATCGGAG
  AGGCTCTGGCCCGTCAGGAGCTCTTTGTCTTCACGGCCTTGCTACTGCAGAGGTTTGACTTGGATGTGTC
  AGATGATAAACAACTGCCCCGCCTGGAGGGTGATCCCAAGGTAGTCTTTCTGATCGACCCTTTCAAAGTA
  AAGATCACGGTGCGCCAGGCATGGATGGATGCACAGGCTGAGGTTAGCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204575 representing NM_012753
  Red=Cloning site Green=Tags(s)
  
  MWELVGLLLLILAYFFWVKSKTPGAKLPRSLPSLPLVGSLPFLPRRGHMHVNFFKLQEKYGPIYSLRLGT
  TTTVIIGHYQLAREVLIKKGKEFSGRPQMVTQSLLSDQGKGVAFADAGSSWHLHRKLVFSTFSLFKDGQK
  LEKLICQEAKSLCDMMLAHDKESIDLSTPIFMSVTNIICAICFNISYEKNDPKLTAIKTFTEGIVDATGD
  RNLVDIFPWLTIFPNKGLEVIKGYAKVRNEVLTGIFEKCREKFDSQSISSLTDILIQAKMNSDNNNSCEG
  RDPDVFSDRHILATVGDIFGAGIETTTTVLKWILAFLVHNPEVKKKIQKEIDQYVGFSRTPTFNDRSHLL
  MLEATIREVLRIRPVAPMLIPHKANVDSSIGEFTVPKDTHVVVNLWALHHDENEWDQPDQFMPERFLDPT
  GSHLITPTQSYLPFGAGPRSCIGEALARQELFVFTALLLQRFDLDVSDDKQLPRLEGDPKVVFLIDPFKV
  KITVRQAWMDAQAEVST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012753
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012753.1, NM_012753.2, NP_036885.1
• RefSeq Size: 1722
• RefSeq ORF: 1524
• Locus ID: 25146
• MW: 57.3 kDa
• Gene Summary: steroid hydroxylase that comprises a main component of the steroidogenic pathway; activity culminates in corticosteriod and androgen production in adrenal gland and sex steroid production in gonads [RGD, Feb 2006]