Ptger1 (NM_013100) Rat Tagged ORF Clone

CAT#: RR204660

Ptger1 (Myc-DDK-tagged ORF) - Rat prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptger1
• Synonyms: EP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204660 representing NM_013100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCTACGGGCTTAACCTGAGCCTAGTGGATGAGGCAACAACGTGTGTAACACCCAGGGTCCCCA
  ATACATCTGTGGTGCTGCCAACAGGCGGTAACGGCACATCACCAGCGCTGCCTATCTTCTCCATGACGCT
  GGGTGCTGTGTCCAACGTGCTGGCGCTGGCGCTGCTGGCCCAGGTTGCAGGCAGACTGCGGCGCCGCCGC
  TCGACTGCCACCTTCCTGTTGTTCGTCGCCAGCCTGCTTGCCATCGACCTAGCAGGCCATGTGATCCCGG
  GCGCCTTGGTGCTTCGCCTGTATACTGCAGGACGTGCGCCCGCTGGCGGGGCCTGTCATTTCCTGGGCGG
  CTGCATGGTCTTCTTTGGCCTGTGCCCACTTTTGCTTGGCTGTGGCATGGCCGTGGAGCGCTGCGTGGGT
  GTCACGCAGCCGCTGATCCACGCGGCGCGCGTGTCCGTAGCCCGCGCACGCCTGGCACTAGCCCTGCTGG
  CCGCCATGGCTTTGGCAGTGGCGCTGCTGCCACTAGTGCACGTGGGTCACTACGAGCTACAGTACCCTGG
  CACTTGGTGTTTCATTAGCCTTGGGCCTCCTGGAGGTTGGCGCCAGGCGTTGCTTGCGGGCCTCTTCGCC
  GGCCTTGGCCTGGCTGCGCTCCTTGCCGCACTAGTGTGTAATACGCTCAGCGGCCTGGCGCTCCTTCGTG
  CCCGCTGGAGGCGGCGTCGCTCTCGACGTTTCCGAGAGAACGCAGGTCCCGATGATCGCCGGCGCTGGGG
  GTCCCGTGGACTCCGCTTGGCCTCCGCCTCGTCTGCGTCATCCATCACTTCAACCACAGCTGCCCTCCGC
  AGCTCTCGGGGAGGCGGCTCCGCGCGCAGGGTTCACGCACACGACGTGGAAATGGTGGGCCAGCTCGTGG
  GCATCATGGTGGTTTCGTGCATCTGCTGGAGCCCCCTGCTGGTATTGGTGGTGTTGGCCATCGGGGGCTG
  GAACTCTAACTCCCTGCAGCGGCCGCTCTTTCTGGCTGTACGCCTCGCGTCGTGGAACCAGATCCTGGAC
  CCATGGGTGTACATCCTGCTGCGCCAGGCTATGCTGCGCCAACTTCTTCGCCTCCTACCCCTGAGGGTTA
  GTGCCAAGGGTGGTCCAACGGAGCTGAGCCTAACCAAGAGTGCCTGGGAGGCCAGTTCACTGCGTAGCTC
  CCGGCACAGTGGCTTCAGCCACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204660 representing NM_013100
  Red=Cloning site Green=Tags(s)
  
  MSPYGLNLSLVDEATTCVTPRVPNTSVVLPTGGNGTSPALPIFSMTLGAVSNVLALALLAQVAGRLRRRR
  STATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCVG
  VTQPLIHAARVSVARARLALALLAAMALAVALLPLVHVGHYELQYPGTWCFISLGPPGGWRQALLAGLFA
  GLGLAALLAALVCNTLSGLALLRARWRRRRSRRFRENAGPDDRRRWGSRGLRLASASSASSITSTTAALR
  SSRGGGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWNSNSLQRPLFLAVRLASWNQILD
  PWVYILLRQAMLRQLLRLLPLRVSAKGGPTELSLTKSAWEASSLRSSRHSGFSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013100
• ORF Size: 1215 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013100.1, NP_037232.1
• RefSeq Size: 2216
• RefSeq ORF: 1218
• Locus ID: 25637
• MW: 81.2 kDa
• Gene Summary: binds prostaglandin E2; plays a role in G-protein coupled receptor signaling and Ca2+ mobilization [RGD, Feb 2006]