Itch (NM_001005887) Rat Tagged ORF Clone

CAT#: RR204734

Itch (Myc-DDK-tagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204734 representing NM_001005887
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAGTCTGACCATGAAATCTCAACTTCAGATTACTGTCATCTCAGCCAAACTTAAAGAAAATAAAA
  AGAATTGGTTTGGACCAAGTCCTTATGTAGAAGTCACAGTAGATGGGCAGTCAAAGAAGACAGAAAAATG
  CAACAATACAAACAGCCCCAAGTGGAAGCAGCCCCTCACAGTTATCGTTACCCCTATGAGTAAATTATGT
  TTTCGTGTGTGGAGTCACCAGACCCTGAAATCTGATGTTTTATTGGGAACTGCTGGATTAGATATTTATG
  AAACATTAAAGTCAAACAATATGAAACTTGAGGAAGTAGTTATGACTTTGCAGCTTGTAGGTGACAAAGA
  GCCAACAGAGACAATGGGAGATTTGTCAGTTTGTCTTGATGGGCTGCAAGTAGAAGCTGAAGTTGTTACT
  AATGGTGAAACTTCATGCTCAGAGGGTGCTACACAGAATGACGCTGGCTGCAGACCCAAAGATGAAACAA
  GAGTGAGCACAAATGGATCAGAGGACCCTGAGGTTGCAGCCTCGGGGGAGAACAAGAGGGCCAATGGGAA
  CAATTCTCCATCCCTTTCAAATGGTGGTTTTAAGCCTTCTAGACCTCCTAGACCTTCAAGACCACCTCCA
  CCCACCCCACGAAGACCAGCTTCTGTCAATGGCTCACCATCCACGAACTCTGACAGCGATGGATCTAGCA
  CAGGCTCTTTGCCACCCACAAATACAAATGTAAATTCAAGTGCATCGGAAGGAGCAGCATCTGGGTTAAT
  AATTCCTCGTACCATCTCTGGGGGCTCAGGCCCTAGGCCGCTGAATACTGTCAGTCAAGCTCCCCTCCCA
  CCTGGGTGGGAGCAGAGAGTAGATCAGCATGGGCGTGCTTACTATGTAGACCATGTTGAAAAGCGAACAA
  CACGGGATAGACCGGAACCTCTACCTCCTGGCTGGGAACGGCGTGTGGACAATATGGGACGTATTTATTA
  TGTTGACCATTTCACGAGAACCACAACATGGCAGAGGCCGACCTTGGAATCTGTCCGGAACTATGAGCAG
  TGGCAGCTCCAGCGTAGTCAACTTCAGGGAGCAATGCAGCAGTTTAACCAGAGATTCATCTTTGGGAATC
  AAGATTTGTTTGCTACATCACAAAACAAAGAGTTTGATCCCCTTGGTCCTTTGCCCCCTGGATGGGAGAA
  GAGAACTGATAGCAACGGCAGAGTGTATTTTGTCAACCACAACACTCGGATTACACAGTGGGAAGACCCC
  AGAAGCCAAGGTCAGTTAAATGAAAAGCCCTTACCAGAAGGCTGGGAAATGCGATTCACAGTGGATGGAA
  TTCCATATTTTGTGGACCACAATAGAAGAGCAACTACCTACATAGATCCACGCACAGGAAAGTCAGCCTT
  AGACAACGGACCCCAGATAGCCTATGTTCGGGACTTCAAGGCAAAAGTTCAGTATTTCCGGTTCTGGTGT
  CAGCAACTGGCCATGCCACAGCATATAAAGATCACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTC
  AACAGATCATGAGCTTCAGTCCGCAAGATCTGAGAAGACGTTTGTGGGTGATTTTCCCAGGAGAAGAAGG
  TTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTAT
  TGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCCTGCAGATAAACCCCGCTTCTTATATCAACCCAG
  ACCACCTGAAATACTTCCGTTTTATTGGCAGATTTATTGCCATGGCTCTATTCCATGGGAAATTCATAGA
  TACCGGATTTTCTTTACCATTCTATAAACGTATCTTGAATAAACCAGTGGGACTTAAGGATTTAGAATCT
  ATCGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAAGAAAACAATATCGAGGAATGTGGTTTGGAAA
  TGTACTTCTCAGTTGATAAAGAAATTCTGGGTGAAATTAAGAGTCATGATTTGAAACCCAATGGTGGCAA
  TATTCTGGTGACAGAAGAAAATAAGGAAGAGTACATCAGGATGGTCGCTGAGTGGAGGTTGTCTCGGGGA
  GTTGAAGAGCAGACACAAGCTTTCTTTGAGGGCTTTAATGAAATTCTTCCTCAGCAATATTTGCAATATT
  TTGATGCGAAGGAATTAGAGGTCCTTTTGTGTGGGATGCAGGAGATCGATTTGAATGACTGGCAGAGACA
  CGCCATCTACCGTCACTATACCAGGACGAGCAAGCAGATCGTGTGGTTTTGGCAGTTTGTTAAAGAAATT
  GATAACGAGAAGAGGATGCGACTTCTGCAGTTTGTTACCGGAACCTGCCGATTGCCAGTGGGAGGATTTG
  CTGACCTCATGGGGAGTAATGGACCACAGAAGTTCTGCATCGAAAAAGTTGGCAAAGAAAATTGGTTACC
  CAGAAGCCATACTTGTTTTAACCGCCTGGACCTCCCACCCTACAAGAGCTATGAGCAACTGAAGGAAAAG
  CTGTTATTTGCCATTGAAGAAACTGAAGGATTTGGACAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204734 representing NM_001005887
  Red=Cloning site Green=Tags(s)
  
  MGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPMSKLC
  FRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVT
  NGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPP
  PTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPRTISGGSGPRPLNTVSQAPLP
  PGWEQRVDQHGRAYYVDHVEKRTTRDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQ
  WQLQRSQLQGAMQQFNQRFIFGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDP
  RSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWC
  QQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMY
  CLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLES
  IDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRG
  VEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIVWFWQFVKEI
  DNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEK
  LLFAIEETEGFGQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005887
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005887.1, NP_001005887.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 2565 bp
• Locus ID: 311567
• Cytogenetics: 3q41
• MW: 97.7 kDa