Itch (NM_001005887) Rat Untagged Clone

CAT#: RN204734

Itch (untagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug)


  "NM_001005887" in other vectors (3)

Reconstitution Protocol

USD 1,150.00

6 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Itch
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204734 representing NM_001005887
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTAGTCTGACCATGAAATCTCAACTTCAGATTACTGTCATCTCAGCCAAACTTAAAGAAAATAAAA
AGAATTGGTTTGGACCAAGTCCTTATGTAGAAGTCACAGTAGATGGGCAGTCAAAGAAGACAGAAAAATG
CAACAATACAAACAGCCCCAAGTGGAAGCAGCCCCTCACAGTTATCGTTACCCCTATGAGTAAATTATGT
TTTCGTGTGTGGAGTCACCAGACCCTGAAATCTGATGTTTTATTGGGAACTGCTGGATTAGATATTTATG
AAACATTAAAGTCAAACAATATGAAACTTGAGGAAGTAGTTATGACTTTGCAGCTTGTAGGTGACAAAGA
GCCAACAGAGACAATGGGAGATTTGTCAGTTTGTCTTGATGGGCTGCAAGTAGAAGCTGAAGTTGTTACT
AATGGTGAAACTTCATGCTCAGAGGGTGCTACACAGAATGACGCTGGCTGCAGACCCAAAGATGAAACAA
GAGTGAGCACAAATGGATCAGAGGACCCTGAGGTTGCAGCCTCGGGGGAGAACAAGAGGGCCAATGGGAA
CAATTCTCCATCCCTTTCAAATGGTGGTTTTAAGCCTTCTAGACCTCCTAGACCTTCAAGACCACCTCCA
CCCACCCCACGAAGACCAGCTTCTGTCAATGGCTCACCATCCACGAACTCTGACAGCGATGGATCTAGCA
CAGGCTCTTTGCCACCCACAAATACAAATGTAAATTCAAGTGCATCGGAAGGAGCAGCATCTGGGTTAAT
AATTCCTCGTACCATCTCTGGGGGCTCAGGCCCTAGGCCGCTGAATACTGTCAGTCAAGCTCCCCTCCCA
CCTGGGTGGGAGCAGAGAGTAGATCAGCATGGGCGTGCTTACTATGTAGACCATGTTGAAAAGCGAACAA
CACGGGATAGACCGGAACCTCTACCTCCTGGCTGGGAACGGCGTGTGGACAATATGGGACGTATTTATTA
TGTTGACCATTTCACGAGAACCACAACATGGCAGAGGCCGACCTTGGAATCTGTCCGGAACTATGAGCAG
TGGCAGCTCCAGCGTAGTCAACTTCAGGGAGCAATGCAGCAGTTTAACCAGAGATTCATCTTTGGGAATC
AAGATTTGTTTGCTACATCACAAAACAAAGAGTTTGATCCCCTTGGTCCTTTGCCCCCTGGATGGGAGAA
GAGAACTGATAGCAACGGCAGAGTGTATTTTGTCAACCACAACACTCGGATTACACAGTGGGAAGACCCC
AGAAGCCAAGGTCAGTTAAATGAAAAGCCCTTACCAGAAGGCTGGGAAATGCGATTCACAGTGGATGGAA
TTCCATATTTTGTGGACCACAATAGAAGAGCAACTACCTACATAGATCCACGCACAGGAAAGTCAGCCTT
AGACAACGGACCCCAGATAGCCTATGTTCGGGACTTCAAGGCAAAAGTTCAGTATTTCCGGTTCTGGTGT
CAGCAACTGGCCATGCCACAGCATATAAAGATCACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTC
AACAGATCATGAGCTTCAGTCCGCAAGATCTGAGAAGACGTTTGTGGGTGATTTTCCCAGGAGAAGAAGG
TTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTAT
TGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCCTGCAGATAAACCCCGCTTCTTATATCAACCCAG
ACCACCTGAAATACTTCCGTTTTATTGGCAGATTTATTGCCATGGCTCTATTCCATGGGAAATTCATAGA
TACCGGATTTTCTTTACCATTCTATAAACGTATCTTGAATAAACCAGTGGGACTTAAGGATTTAGAATCT
ATCGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAAGAAAACAATATCGAGGAATGTGGTTTGGAAA
TGTACTTCTCAGTTGATAAAGAAATTCTGGGTGAAATTAAGAGTCATGATTTGAAACCCAATGGTGGCAA
TATTCTGGTGACAGAAGAAAATAAGGAAGAGTACATCAGGATGGTCGCTGAGTGGAGGTTGTCTCGGGGA
GTTGAAGAGCAGACACAAGCTTTCTTTGAGGGCTTTAATGAAATTCTTCCTCAGCAATATTTGCAATATT
TTGATGCGAAGGAATTAGAGGTCCTTTTGTGTGGGATGCAGGAGATCGATTTGAATGACTGGCAGAGACA
CGCCATCTACCGTCACTATACCAGGACGAGCAAGCAGATCGTGTGGTTTTGGCAGTTTGTTAAAGAAATT
GATAACGAGAAGAGGATGCGACTTCTGCAGTTTGTTACCGGAACCTGCCGATTGCCAGTGGGAGGATTTG
CTGACCTCATGGGGAGTAATGGACCACAGAAGTTCTGCATCGAAAAAGTTGGCAAAGAAAATTGGTTACC
CAGAAGCCATACTTGTTTTAACCGCCTGGACCTCCCACCCTACAAGAGCTATGAGCAACTGAAGGAAAAG
CTGTTATTTGCCATTGAAGAAACTGAAGGATTTGGACAAGAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001005887
Insert Size 2565 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001005887.1, NP_001005887.1
RefSeq Size 2565 bp
RefSeq ORF 2565 bp
Locus ID 311567
Cytogenetics 3q41

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.