Hal (NM_017159) Rat Tagged ORF Clone

CAT#: RR204895

Hal (Myc-DDK-tagged ORF) - Rat histidine ammonia lyase (Hal), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hal
• Synonyms: histidase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204895 representing NM_017159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAGGTACACGGTGCACGTGCGTGGGGAATGGCTGGCGGTACCCTGCCAGGACGGGAAGCTCAGTG
  TTGGCTGGCTGGGCCGCGAGGCTGTGCGGCGCTACATGAAGAACAAGCCGGACAACGGGGGCTTCACCTC
  GGTGGATGAAGTGCGATTCCTTGTGCGCCGGTGCAAGGGCCTGGGCCTGTTGGACAATGAGGACCTGCTG
  GAGGTGGCCCTGGAGGACAATGAGTTCGTAGAAGTGGTGATTGAAGGCGATGTAATGTCTCCTGATTTCA
  TTCCATCACAGCCAGAAGGCGTTTTCCTTTACAGCAAATACCGGGAGCCTGAAAAGTACATTGCCTTGGA
  TGGGGACAGTCTGAGCACGGAGGATCTGGTCAACCTAGGCAAGGGACATTACAAGATAAAGCTCACTTCA
  ATAGCTGAGAAGAAAGTGCAGCAGTCCCGGGAAGTCATTGACAGCATCATAAAAGAAAGAACAGTTGTTT
  ACGGCATCACCACGGGTTTTGGAAAATTTGCCAGAACTGTGATCCCTGCCAATAAGCTACAGGAGCTTCA
  GGTCAACTTAGTCCGTTCCCATTCTTCAGGTGTTGGGAAGCCACTCAGTCCTGAGAGGTGTCGGATGCTC
  TTGGCTTTGAGGATCAATGTCTTAGCCAAAGGATACAGTGGGATTTCCTTGGAGACCCTGAAACAAGTGA
  TTGAAGTATTTAATGCCTCCTGCCTGTCCTATGTTCCAGAGAAAGGGACCGTTGGTGCCAGCGGAGACCT
  TGCCCCACTCTCCCATCTTGCTTTGGGGCTCATTGGAGAAGGAAAGATGTGGTCCCCAAAGAGTGGCTGG
  GCCGATGCCAAATATGTCTTAGAAGCCCACGGATTGAAACCAATTGTTCTGAAACCAAAAGAAGGCCTGG
  CACTCATCAACGGGACACAGATGATCACTTCCCTGGGCTGTGAAGCCGTGGAGCGAGCCAGCGCCATTGC
  CCGGCAAGCGGATATCGTGGCTGCCTTGACCCTCGAGGTGCTGAAGGGCACCACCAAAGCCTTCGATACC
  GACATCCACGCCGTCCGCCCTCACCGCGGGCAAATTGAAGTTGCTTTCCGGTTCCGGTCCCTCTTGGACT
  CAGATCACCACCCGTCAGAAATTGCAGAAAGCCACAGGTTCTGTGATCGTGTTCAGGATGCGTACACCTT
  ACGCTGCTGTCCACAGGTCCACGGTGTGGTGAACGATACAATTGCCTTTGTGAAGGACATCATCACCACA
  GAACTGAACAGTGCCACAGACAATCCTATGGTCTTTGCCAGTCGAGGGGAGACAATTTCGGGAGGAAACT
  TCCACGGTGAATACCCAGCCAAAGCCCTGGACTATTTGGCCATTGGCGTCCATGAACTTGCGGCAATTAG
  TGAAAGAAGAATCGAAAGGCTGTGTAATCCTTCCCTCAGTGAGCTGCCCGCCTTCCTGGTGGCTGAAGGA
  GGTCTCAATTCTGGATTCATGATAGCCCACTGTACCGCAGCAGCCCTGGTGTCTGAGAGCAAGGCTCTGT
  GCCACCCTTCATCTGTGGACTCACTCTCTACCAGTGCTGCCACGGAGGACCACGTCTCCATGGGAGGATG
  GGCAGCCAGGAAAGCTCTCAGGGTCATCGAGCACGTGGAACAAGTGCTGGCCATTGAGCTTCTCGCGGCC
  TGCCAGGGCATAGAGTTTCTGCGCCCCTTGAAAACAACCACTCCGCTGGAGAAGGTGTACGACCTGGTGC
  GCTCCGTAGTAAGGCCCTGGATCAAAGATCGCTTCATGGCCCCGGACATCGAGGCAGCACACAGGTTGCT
  TCTTGACCAGAAGGTTTGGGAGGTGGCTGCCCCGTACATCGAGAAATACAGAATGGAGCACATTCCGGAA
  TCCAGACCGCTTTCTCCAACAGCGTTTTCACTAGAATCTCTGAGGAAGAATTCAGCCACAATCCCAGAGT
  CTGACGATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204895 representing NM_017159
  Red=Cloning site Green=Tags(s)
  
  MPRYTVHVRGEWLAVPCQDGKLSVGWLGREAVRRYMKNKPDNGGFTSVDEVRFLVRRCKGLGLLDNEDLL
  EVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGHYKIKLTS
  IAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRML
  LALRINVLAKGYSGISLETLKQVIEVFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGW
  ADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDT
  DIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITT
  ELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEG
  GLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAA
  CQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPE
  SRPLSPTAFSLESLRKNSATIPESDDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017159
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017159.1, NP_058855.1
• RefSeq Size: 2136
• RefSeq ORF: 1974
• Locus ID: 29301
• MW: 72.3 kDa
• Gene Summary: catalyzes the conversion of histidine to urocanic acid [RGD, Feb 2006]