Prkci (NM_032059) Rat Tagged ORF Clone

CAT#: RR205182

Prkci (Myc-DDK-tagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkci
• Synonyms: Pkcl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205182 representing NM_032059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACCCAGAGGGACAGCAGCACCATGTCTCACACGGTCGCGTGCGGCGGCGGCGGGGACCATTCCC
  ACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATTATGATAACACACTTCGAGCCTTCCATCTCCTT
  TGAAGGACTTTGCAGTGAGGTTCGAGATATGTGTTCTTTTGACAATGAGCAGCCATTCACCATGAAATGG
  ATAGATGAGGAAGGAGACCCGTGCACAGTGTCTTCTCAGTTGGAGTTGGAAGAGGCTTTCAGGCTGTATG
  AGTTGAACAAGGATTCTGAACTCCTGATCCACGTGTTCCCGTGTGTACCAGAGCGTCCTGGAATGCCTTG
  CCCAGGGGAAGACAAGTCCATTTACCGCAGAGGGGCGCGCAGGTGGAGAAAGCTGTATTGTGCAAATGGC
  CACACTTTTCAAGCCAAACGCTTTAACAGGCGTGCCCACTGTGCCATCTGCACAGACAGGATCTGGGGAC
  TTGGACGACAAGGATATAAATGCATCAACTGCAAACTGCTGGTTCATAAGAAGTGCCACAAGCTTGTCAC
  AATTGAGTGTGGGCGGCATTCTTTGCCACCGGAACCCATGATGCCAATGGACCAGTCATCCATGCACCCA
  GACCACACACAGACAGTAATTCCATATAATCCTTCAAGTCATGAGAGTTTGGACCAAGTTGGTGAAGAAA
  AGGAGGCAATGAACACCAGGGAGAGTGGGAAGGCGTCATCAAGCTTAGGTCTCCAGGATTTCGATTTGCT
  TCGAGTTATAGGGAGAGGAAGTTACGCCAAAGTACTGCTGGTTCGATTAAAAAAGACAGATCGCATTTAT
  GCAATGAAGGTTGTGAAGAAAGAGCTCGTCAATGACGATGAGGATATTGATTGGGTACAGACAGAAAAGC
  ATGTGTTTGAGCAGGCGTCCAATCACCCTTTCCTTGTTGGTCTGCATTCCTGCTTCCAGACAGAAAGCAG
  GCTGTTTTTTGTCATAGAATATGTGAATGGAGGGGATCTCATGTTTCATATGCAGCGGCAAAGAAAACTT
  CCTGAAGAACATGCCAGGTTTTACTCAGCAGAAATCAGTCTAGCACTAAATTATCTTCATGAGCGAGGGA
  TAATTTATAGAGATTTGAAGTTGGACAATGTACTGCTGGACTCTGAAGGACACATCAAACTCACTGACTA
  CGGCATGTGTAAGGAAGGATTACGGCCCGGAGATACAACCAGCACCTTCTGTGGGACTCCCAATTACATT
  GCTCCTGAGATCTTAAGAGGAGAAGACTATGGCTTCAGCGTTGACTGGTGGGCTCTTGGAGTACTCATGT
  TTGAGATGATGGCGGGAAGGTCTCCATTTGATATCGTTGGGAGCTCTGACAATCCTGACCAAAACACAGA
  GGATTATCTGTTCCAAGTCATTTTGGAGAAGCAGATTCGCATACCGCGCTCCCTGTCTGTGAAAGCAGCA
  AGTGTGCTGAAGAGTTTCCTCAACAAGGACCCAAAGGAACGATTGGGTTGTCACCCTCAAACTGGATTTG
  CTGACATCCAAGGACACCCATTCTTCCGTAATGTGGATTGGGACATGATGGAGCAGAAGCAAGTGGTTCC
  GCCCTTTAAACCAAACATTTCTGGAGAATTTGGTTTGGATAACTTTGACTCCCAGTTTACCAACGAACCA
  GTCCAGCTCACTCCAGATGATGATGACATCGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTCGAGT
  ATATCAACCCTCTCTTGATGTCTGCAGAAGAGTGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205182 representing NM_032059
  Red=Cloning site Green=Tags(s)
  
  MPTQRDSSTMSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKW
  IDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANG
  HTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQSSMHP
  DHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIY
  AMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKL
  PEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYI
  APEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAA
  SVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEP
  VQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032059
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032059.1, NP_114448.1
• RefSeq Size: 4501
• RefSeq ORF: 1791
• Locus ID: 84006
• MW: 68.3 kDa
• Gene Summary: member of the atypical subgroup of PKCs that do not contain the calcium binding domain and possess only one cysteine-rich region; plays a critical role in controlling cell growth and cell survival; enhances Rab2-mediated vesicle budding [RGD, Feb 2006]