Bcl2l14 (NM_001024338) Rat Tagged ORF Clone

CAT#: RR205191

Bcl2l14 (Myc-DDK-tagged ORF) - Rat Bcl2-like 14 (apoptosis facilitator) (Bcl2l14), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcl2l14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205191 representing NM_001024338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAGCACCAGTGTGTACGACCTGGAAGATATCCCCCTGGAAGACGATGATCCGAACAGCATAGAGT
  TCAAAATCCTGGCCTTCTATGCCAGACACCATGTCTTCAAGAGCACCCCGGCTGTCTTCTCCCCCAAGCT
  CTCCAGGACCAGAAGTTTGTCCCAGAAAGCACTGGGGACTTGGTCAACTGACTCCTGGACACAGATATCG
  TTGCCCTGTAGAGATTCCCTCTCCAGTGAAAAGCACATAAGCTTGGGCAAGAAGAAGTCTTCTTGGAGAA
  CACTCTTCAGAGTAACAGAGAAGGAGGAAGAGCCGCCGAGCTCCCCAAAGGAGATCCATGCTCAGGGCCC
  CTTCCCGGTAGAGCGGCAGGGTAGGAACCAGCACTGGTCCAGATCCCTGTCCAGCGTGGAGCAGTGCCTG
  GAGAGTGAAGCTGTGGATTCCAAAGTTGCCTGTATTGCTAACAGAGTGGCTGAAATAGTTTACTCCTGGC
  CACCACCAGATGACAGCCAGAGCCAGGGAGGAGGCAAGTTCAAAGAGAGTGTCCCAAAGATTCTGCACTT
  TGAATTGCAAGGCCCTCAACTGAGACCCTGCGACTCGAAGAAAGATGGAGAAGACCAAATAATAAACAGG
  ATTGTTGAACTGCTGAAATACTCGGGGGATCAGTTGGGAAGAGAGATAAAGAGAGACAAGGCTTTGGTGA
  GCATCTTCCAGGAAGGGCTGTCCTACTCGGTGTTCAGGACCATCACAGACCTGTTCCTGAGGGACGTGGA
  CACCAGAGGAGAGTCAGAGATCAAAGCTCAGGGCTTCAAGGCTGCCCTTGCAATAGATGCCATAGCCAAG
  CTCACCGCCATCGACAACCACCCAATGAACAGGATGCTGGGCTTTGGGACCAAGTACCTAAGAGAGTACT
  TCTCCCCCTGGGTCCAGCAGAACGGTGGATGGGAAAAAATACTTGGGCTATCACATGAAGAAGTAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205191 representing NM_001024338
  Red=Cloning site Green=Tags(s)
  
  MCSTSVYDLEDIPLEDDDPNSIEFKILAFYARHHVFKSTPAVFSPKLSRTRSLSQKALGTWSTDSWTQIS
  LPCRDSLSSEKHISLGKKKSSWRTLFRVTEKEEEPPSSPKEIHAQGPFPVERQGRNQHWSRSLSSVEQCL
  ESEAVDSKVACIANRVAEIVYSWPPPDDSQSQGGGKFKESVPKILHFELQGPQLRPCDSKKDGEDQIINR
  IVELLKYSGDQLGREIKRDKALVSIFQEGLSYSVFRTITDLFLRDVDTRGESEIKAQGFKAALAIDAIAK
  LTAIDNHPMNRMLGFGTKYLREYFSPWVQQNGGWEKILGLSHEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024338
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024338.1, NP_001019509.1
• RefSeq Size: 1608 bp
• RefSeq ORF: 981 bp
• Locus ID: 500348
• Cytogenetics: 4q43
• MW: 36.9 kDa
• Gene Summary: Plays a role in apoptosis. [UniProtKB/Swiss-Prot Function]