Bace2 (NM_001002802) Rat Tagged ORF Clone

CAT#: RR205199

Bace2 (Myc-DDK-tagged ORF) - Rat beta-site APP-cleaving enzyme 2 (Bace2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bace2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205199 representing NM_001002802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGCTGCTTCGAGCACTCTTGCTCCCACTGCTGGCGCAGTGGCTCTTGCGCGCGGTCCCCGTGC
  TGGCCCCGGCGCCCTTCACGCTTCCCCTCCAAGTGGCCGGGGCCGCGAACCACAGAGCCTCAACTGTTCC
  CGGGCTCGGGACCCCCGAGTTGCCCCGGGCTGATGGCCTGGCCCTCGCACTGGAGCCTGCCAGGGCTACC
  GCCAACTTCTTGGCTATGGTGGACAATCTTCAGGGGGACTCTGGCCGCGGCTACTACCTGGAGATGCTGA
  TTGGGACCCCTCCGCAAAAGGTACGGATTCTTGTGGACACCGGAAGCAGTAACTTCGCAGTGGCAGGTGC
  CCCACACTCCTACATAGACACCTACTTTGACTCAGAGAGCTCCAGCACATACCACTCCAAGGGCTTTGAG
  GTCACGGTGAAGTACACACAGGGAAGCTGGACTGGATTTGTTGGTGAGGACCTTGTCACCATCCCAAAAG
  GCTTCAACAGCTCTTTCTTGGTCAATATTGCCACTATTTTCGAGTCTGAGAATTTCTTTTTGCCTGGGAT
  TAAGTGGAATGGAATCCTTGGACTCGCTTATGCTGCCTTGGCCAAGCCATCAAGCTCTCTGGAGACTTTT
  TTTGATTCCCTGGTGGCCCAAGCAAAAATTCCGGACATTTTCTCCATGCAGATGTGCGGGGCTGGATTGC
  CAGTAGCTGGATCTGGTACCAACGGAGGTAGTCTTGTCCTGGGTGGGATTGAACCAAGTTTGTATAAAGG
  AGATATATGGTATACCCCAATTAAAGAGGAATGGTACTATCAAATAGAGATCTTGAAATTGGAAATTGGA
  GGCCAGAGCCTCAACCTGGACTGCAGAGAGTATAACGCAGACAAGGCCATTGTGGACAGCGGCACCACAC
  TCCTGCGCCTGCCCCAGAAGGTGTTTGATGCAGTGGTGGAAGCCGTGGCACGCACATCTCTGATTCCAGA
  GTTTTCTGATGGCTTCTGGACGGGGGCCCAGCTGGCATGCTGGACAAATTCTGAAACTCCATGGGCGTAT
  TTCCCTAAGATTTCTATCTACCTGAGAGATGAGAACGCCAGTCGCTCCTTCCGAATCACCATTCTGCCGC
  AGCTCTACATTCAGCCCATGATGGGAGCTGGTTTCAATTATGAGTGCTACCGTTTTGGTATCTCCTCTTC
  CACAAATGCGCTGGTGATTGGTGCCACCGTGATGGAGGGATTCTACGTGGTCTTTGACAGAGCTCAGAGG
  AGGGTGGGCTTTGCAGTGAGTCCCTGTGCAGAGATTGCAGGTACCACAGTGTCTGAAATTTCTGGGCCCT
  TTTCCACGGAAGACATAGCCAGCAACTGTGTTCCAGCACAGGCTCTGAATGAGCCCATCTTGTGGATTGT
  GTCCTATGCCCTGATGAGTGTGTGTGGAGCCATTCTCCTGGTTCTGATCCTCCTGCTGCTGTTCCCGCTG
  CACTGCCGACACGCCCCCCGAGACCCTGAGGTGGTTAACGATGAATCCTCACTAGTCAGACATCGCTGGA
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205199 representing NM_001002802
  Red=Cloning site Green=Tags(s)
  
  MGALLRALLLPLLAQWLLRAVPVLAPAPFTLPLQVAGAANHRASTVPGLGTPELPRADGLALALEPARAT
  ANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVRILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFE
  VTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETF
  FDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIG
  GQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAY
  FPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQR
  RVGFAVSPCAEIAGTTVSEISGPFSTEDIASNCVPAQALNEPILWIVSYALMSVCGAILLVLILLLLFPL
  HCRHAPRDPEVVNDESSLVRHRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002802
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002802.1, NP_001002802.1
• RefSeq Size: 1545 bp
• RefSeq ORF: 1545 bp
• Locus ID: 288227
• Cytogenetics: 11q12
• MW: 55.9 kDa