Egr1 (NM_012551) Rat Tagged ORF Clone

CAT#: RR205200

Egr1 (Myc-DDK-tagged ORF) - Rat early growth response 1 (Egr1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egr1
• Synonyms: Krox-24; Ngf1; Ngfi; NGFI-A; zif-268
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205200 representing NM_012551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGACAACTACCCCAAACTGGAGGAGATGATGCTGCTGAGCAACGGGGCTCCCCAGTTCCTCGGTGCTG
  CCGGAACCCCAGAGGGCAGCGGCGGCAATAACAGCAGCAGCAGCAGCAGCAGCAGCAGCGGGGGCGGTGG
  TGGGGGCGGCAGCAACAGCGGCAGCAGCGCTTTCAATCCTCAAGGGGAGCCGAGCGAACAACCCTACGAG
  CACCTGACCACAGAGTCCTTTTCTGACATCGCTCTGAATAACGAGAAGGCGCTGGTGGAGACAAGTTATC
  CCAGCCAAACTACCCGGTTGCCTCCCATCACCTATACTGGCCGCTTCTCCCTGGAGCCTGCACCCAACAG
  TGGCAACACTTTGTGGCCTGAACCCCTTTTCAGCCTAGTCAGTGGCCTTGTGAGCATGACCAACCCTCCA
  ACCTCTTCATCCTCAGCGCCTTCTCCAGCTGCTTCATCGTCTTCCTCTGCCTCCCAGAGCCCACCCCTGA
  GCTGTGCCGTGCCGTCCAACGACAGCAGTCCCATTTACTCAGCTGCACCCACCTTTCCTACTCCCAACAC
  TGACATTTTTCCTGAGCCCCAAAGCCAGGCCTTTCCTGGCTCTGCAGGCACAGCCTTGCAGTACCCGCCT
  CCTGCCTACCCTGCCACCAAGGGTGGTTTCCAGGTTCCCATGATCCCTGACTATCTGTTTCCACAACAAC
  AGGGAGACCTGAGCCTGGGCACCCCAGACCAGAAGCCCTTCCAGGGTCTGGAGAACCGTACCCAGCAGCC
  TTCGCTCACTCCACTATCCACTATCAAAGCCTTCGCCACTCAGTCGGGCTCCCAGGACTTAAAGGCTCTT
  AATAACACCTACCAGTCCCAACTCATCAAACCCAGCCGCATGCGCAAGTACCCCAACCGGCCCAGCAAGA
  CACCCCCCCATGAACGCCCGTATGCTTGCCCTGTTGAGTCCTGCGATCGCCGCTTTTCTCGCTCGGATGA
  GCTTACACGCCACATCCGCATCCATACAGGCCAGAAGCCCTTCCAGTGTCGAATCTGCATGCGTAATTTC
  AGTCGTAGTGACCACCTTACCACCCACATCCGCACCCACACAGGCGAGAAGCCTTTTGCCTGTGACATTT
  GTGGGAGAAAGTTTGCCAGGAGTGATGAACGCAAGAGGCATACCAAAATCCACTTAAGACAGAAGGACAA
  GAAAGCAGACAAAAGTGTCGTGGCCTCCTCAGCTGCCTCTTCCCTCTCTTCCTACCCATCCCCAGTGGCT
  ACCTCCTACCCATCCCCCGCCACCACCTCATTTCCATCCCCAGTGCCCACCTCTTACTCCTCTCCGGGCT
  CCTCTACCTACCCGTCTCCTGCACACAGTGGCTTCCCATCGCCCTCGGTGGCCACCACCTATGCCTCCGT
  CCCACCTGCTTTCCCTGCCCAGGTCAGCACCTTCCAGTCTGCAGGGGTCAGCAACTCCTTCAGCACCTCA
  ACGGGTCTTTCAGACATGACAGCAACCTTTTCTCCTAGGACAATTGAAATTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205200 representing NM_012551
  Red=Cloning site Green=Tags(s)
  
  MDNYPKLEEMMLLSNGAPQFLGAAGTPEGSGGNNSSSSSSSSSGGGGGGGSNSGSSAFNPQGEPSEQPYE
  HLTTESFSDIALNNEKALVETSYPSQTTRLPPITYTGRFSLEPAPNSGNTLWPEPLFSLVSGLVSMTNPP
  TSSSSAPSPAASSSSSASQSPPLSCAVPSNDSSPIYSAAPTFPTPNTDIFPEPQSQAFPGSAGTALQYPP
  PAYPATKGGFQVPMIPDYLFPQQQGDLSLGTPDQKPFQGLENRTQQPSLTPLSTIKAFATQSGSQDLKAL
  NNTYQSQLIKPSRMRKYPNRPSKTPPHERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNF
  SRSDHLTTHIRTHTGEKPFACDICGRKFARSDERKRHTKIHLRQKDKKADKSVVASSAASSLSSYPSPVA
  TSYPSPATTSFPSPVPTSYSSPGSSTYPSPAHSGFPSPSVATTYASVPPAFPAQVSTFQSAGVSNSFSTS
  TGLSDMTATFSPRTIEIC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012551
• ORF Size: 1524 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012551.1, NM_012551.2, NP_036683.1
• RefSeq Size: 1527
• RefSeq ORF: 1527
• Locus ID: 24330
• MW: 53.9 kDa
• Gene Summary: activates transcription of the LH receptor gene; may be involved in synaptic plasticity during REM sleep [RGD, Feb 2006]