Ucp1 (NM_012682) Rat Tagged ORF Clone

CAT#: RR205275

Ucp1 (Myc-DDK-tagged ORF) - Rat uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ucp1
• Synonyms: Ucp; Ucpa; Uncp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205275 representing NM_012682
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGTTCGACAACTTCCGAAGTGCAACCCACCATGGGGGTCAAGATCTTCTCAGCCGGCGTTTCTG
  CCTGCCTAGCAGACATCATCACCTTCCCGCTGGACACCGCCAAAGTCCGCCTTCAGATCCAAGGTGAAGG
  CCAGGCTTCCAGTACTATTAGGTATAAAGGTGTCTTAGGGACCATCACCACCCTGGCCAAGACAGAAGGA
  TTGCCGAAACTGTACAGCGGTCTGCCTGCTGGCATCCAGAGGCAAATCAGCTTTGCTTCCCTCAGGATTG
  GCCTCTACGATACGGTCCAAGAGTACTTCTCTTCAGGGAGAGAAACGCCTGCCTCTTTGGGAAGCAAGAT
  CTCGGCTGGCTTGATGACGGGTGGCGTGGCGGTATTCATTGGGCAGCCCACAGAGGTGGTGAAGGTCAGA
  ATGCAAGCACAAAGCCATCTGCACGGGATCAAACCCCGCTACACTGGGACCTACAATGCTTACAGAGTTA
  TAGCCACCACAGAAAGCTTGTCAACACTGTGGAAAGGGACGACTCCTAATCTAATGAGAAATGTCATCAT
  CAACTGTACAGAGCTGGTGACATATGACCTCATGAAGGGGGCCCTTGTGAACCACCACATACTGGCAGAT
  GACGTCCCCTGCCATTTACTGTCAGCTCTTGTCGCCGGGTTTTGCACCACACTCCTGGCCTCTCCGGTGG
  ATGTGGTAAAAACGAGATTCATCAACTCTCTACCAGGACAGTACCCAAGTGTACCCAGCTGTGCAATGAC
  CATGTACACCAAGGAAGGACCGGCAGCCTTTTTCAAAGGGTTTGCGCCTTCTTTTCTGCGACTCGGATCC
  TGGAACGTCATCATGTTTGTGTGCTTTGAACAGCTGAAGAAAGAGCTGATGAAGTCCCGGCAGACAGTGG
  ACTGCACCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205275 representing NM_012682
  Red=Cloning site Green=Tags(s)
  
  MVSSTTSEVQPTMGVKIFSAGVSACLADIITFPLDTAKVRLQIQGEGQASSTIRYKGVLGTITTLAKTEG
  LPKLYSGLPAGIQRQISFASLRIGLYDTVQEYFSSGRETPASLGSKISAGLMTGGVAVFIGQPTEVVKVR
  MQAQSHLHGIKPRYTGTYNAYRVIATTESLSTLWKGTTPNLMRNVIINCTELVTYDLMKGALVNHHILAD
  DVPCHLLSALVAGFCTTLLASPVDVVKTRFINSLPGQYPSVPSCAMTMYTKEGPAAFFKGFAPSFLRLGS
  WNVIMFVCFEQLKKELMKSRQTVDCTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012682
• ORF Size: 921 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012682.1, NM_012682.2, NP_036814.1
• RefSeq Size: 1194
• RefSeq ORF: 924
• Locus ID: 24860
• MW: 33.7 kDa
• Gene Summary: plays a role in heat production by uncoupling oxidative phosphorylation from the respiratory chain [RGD, Feb 2006]