Chrna10 (NM_022639) Rat Tagged ORF Clone

CAT#: RR205353

Chrna10 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 10 (Chrna10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205353 representing NM_022639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAAGGAGCCACTACCTGGACCTGGGGTTTCTGCTGCTGCTGTTTCTCCCTGCAGAGTGCCTGG
  GAGCTGAGGGGAGACTGGCTCACAAGCTGTTTCGTGACCTGTTTGCCAACTATACAAGTGCTCTGAGACC
  AGTGGCAGATACAGACCAGACTCTAAATGTGACCCTGGAGGTGACATTGTCTCAGATCATTGATATGGAT
  GAACGGAACCAGGTGCTGACCTTGTACCTGTGGATCCGGCAAGAGTGGACAGATGCCTACCTACACTGGG
  ACCCCAAAGCCTATGGTGACCTGGACGCAATCCGAATTCCCAGCCGTCTAGTGTGGCGACCAGACATCGT
  ACTTTACAACAAGGCGGACACGCAGCCACCTGCCTCAGCCAGCACCAACGTGGTTGTGCGGCACGACGGC
  GCTGTGCGCTGGGACGCACCGGCCATCACACGCAGCTCGTGCCGTGTGGACGTGTCTGCCTTCCCTTTTG
  ACGCGCAGCGCTGCGGCCTGACCTTCGGCTCATGGACGCACGGTGGGCACCAGCTGGATGTGCGACCTCG
  GGGCACTTCCGCCAGTCTGGCCGACTTCGTGGAGAACGTTGAATGGCGGGTGCTGGGCATGCCAGCACGC
  AGGCGGGTCCTCACCTATGGCTGCTGCTCTGAGCCCTACCCAGATGTGACCTTTACTCTGTTGCTGCGCC
  GCCGCGCTGCAGCCTACGTGTGCAACCTCCTGCTGCCCTGTGTGTTCATCTCCCTGCTGGCGCCTCTGGC
  CTTCCACCTGCCTGCTGACTCTGGGGAGAAGGTGTCTCTGGGCGTCACCGTGCTCCTGGCGCTCACCGTC
  TTCCAGCTGATCCTGGCCGAGAGCATGCCACCTGCAGAGAGTGTGCCACTCATCGGAAAGTACTATATGG
  CCACTATGACCATGGTCACATTCTCCACAGCACTTACCATCCTCATCATGAATCTGCACTACTGTGGCCC
  TAATGCACATCCAGTGCCCGCCTGGGCTCGGGTTCTCCTGCTGGGACACCTAGCCAAAGGCCTGTGTGTG
  CGGGAACGAGGGGAGCCCTGTGGGCAGTCCAAGCCACTAGAGTCAGCCCCCAGCCTCCAGCCTCCACCAG
  CTTCCCCAGCGGGCCCTTGTCATGAGCCACGGTGTCTATGCCACCAGGAAGCCCTTCTACATCATATAGC
  TTCCATTGCTAGTACCTTCCGCAGCCACCGGGCTGCCCAGCGCCGCCACGAAGATTGGAAGCGTCTGGCT
  AGAGTAATGGACCGCTTTTTCCTAGGCATCTTCTTCTGCATGGCTCTGGTCATGAGCCTCATTGTACTGG
  TGCAAGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205353 representing NM_022639
  Red=Cloning site Green=Tags(s)
  
  MGTRSHYLDLGFLLLLFLPAECLGAEGRLAHKLFRDLFANYTSALRPVADTDQTLNVTLEVTLSQIIDMD
  ERNQVLTLYLWIRQEWTDAYLHWDPKAYGDLDAIRIPSRLVWRPDIVLYNKADTQPPASASTNVVVRHDG
  AVRWDAPAITRSSCRVDVSAFPFDAQRCGLTFGSWTHGGHQLDVRPRGTSASLADFVENVEWRVLGMPAR
  RRVLTYGCCSEPYPDVTFTLLLRRRAAAYVCNLLLPCVFISLLAPLAFHLPADSGEKVSLGVTVLLALTV
  FQLILAESMPPAESVPLIGKYYMATMTMVTFSTALTILIMNLHYCGPNAHPVPAWARVLLLGHLAKGLCV
  RERGEPCGQSKPLESAPSLQPPPASPAGPCHEPRCLCHQEALLHHIASIASTFRSHRAAQRRHEDWKRLA
  RVMDRFFLGIFFCMALVMSLIVLVQAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022639
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022639.1, NP_072161.1
• RefSeq Size: 2172
• RefSeq ORF: 1344
• Locus ID: 64574
• MW: 49.8 kDa
• Gene Summary: alpha10 subunit of the nicotininc acetylcholine receptor (nAChR); may heterodimerize with alpha9 subunit [RGD, Feb 2006]