Parp1 (NM_013063) Rat Tagged ORF Clone

CAT#: RR205456

Parp1 (Myc-DDK-tagged ORF) - Rat poly (ADP-ribose) polymerase 1 (Parp1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parp1
• Synonyms: Adprt; ARTD1; Parp-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205456 representing NM_013063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCCACGGAGAGGCTTTACCGAGTGGAGTACGCTAAGAGCGGGCGCGCCTCTTGCAAGAAAT
  GCAGCGAGAGTATCCCCAAGGACTCGCTCCGCATGGCCATCATGGTGCAGTCACCCATGTTCGATGGAAA
  AGTCCCACACTGGTATCACTTCTCCTGCTTCTGGAAGGTGGGCCACTCCATCCGGCAGCCTGACACAGAG
  GTGGACGGGTTCTCTGAGCTGCGGTGGGATGACCAGCAGAAGGTCAAGAAGACCGCAGAGGCTGGAGGCG
  TGGCAGGCAAAGGCCAGCATGGAGGTGGCGGCAAGGCGGAGAAGACGCTGGGCGACTTTGCGGCAGAGTA
  TGCCAAGTCCAACAGGAGCACATGCAAGGGCTGCATGGAGAAGATAGAGAAGGGTCAGATGCGCCTGTCC
  AAGAAGATGCTGGATCCAGAGAAGCCGCAACTGGGTATGATTGACCGCTGGTACCATCCAACTTGCTTTG
  TCAAGAACCGGGACGAGCTGGGCTTCCGGCCTGAGTACAGTGCCAGCCAGCTCAAGGGCTTTAGCCTCCT
  CTCTGCAGAAGACAAAGAAGCTCTGAAGAAGCAGCTCCCGGCAGTCAAGAGTGAAGGAAAGAGAAAATGT
  GACGAGGTGGATGGAATAGATGAAGTAGCCAAAAAGAAATCTAAAAAAGGAAAGGACAAGGAGAGTAGTA
  AACTGGAAAAGGCGCTCAAGGCTCAGAACGAGCTGGTCTGGAATATCAAAGATGAGCTGAAGAAAGCCTG
  CTCCACCAACGACCTGAAGGAGTTGCTCATCTTCAACCAGCAGCAAGTACCATCTGGAGAGTCCGCAATC
  CTGGACCGAGTTGCTGATGGCATGGCGTTTGGGGCCCTTCTGCCCTGCAAGGAGTGTTCAGGACAGCTGG
  TTTTTAAGAGCGACGCTTATTACTGTACTGGGGATGTCACTGCCTGGACCAAGTGCATGGTCAAGACACA
  GAATCCCAGCCGAAAGGAATGGGTAACTCCAAAGGAATTCCGAGAAATATCCTACCTCAAGAAATTAAAG
  ATCAAAAAGCAGGACCGGCTGTTCCCTCCAGAGAGCAGCGCCCCAGCACCACCAGCACCTCCCGTCTCGA
  TCACCTCCGCACCCACAGCTGTGAACTCCTCTGCACCAGCAGACAAACCCCTGTCTAACATGAAGATCCT
  GACTCTTGGTAAGCTCTCCCAGAACAAGGACGAAGCAAAGGCCATGATTGAGAAACTCGGAGGCAAGCTG
  ACAGGATCTGCCAACAAGGCCTCCTTGTGTATCAGCACTAAGAAGGAGGTGGAGAAGATGAGTAAGAAGA
  TGGAGGAAGTGAAAGCAGCCAATGTTCGAGTCGTGTGTGAGGACTTCCTCCAGGATGTGTCTGCCTCAGC
  TAAGAGCCTCCAAGAGCTGCTCTCAGCCCATAGCTTGTCCTCGTGGGGGGCTGAGGTGAAGGTAGAGCCT
  GGTGAAGTGGTGGTCCCCAAGGGGAAGTCAGCTGCACCCTCCAAGAAGAGCAAGGGTGCTGTCAAGGAGG
  AAGGTGTCAACAAGTCTGAAAAGAGGATGAAACTAACTCTGAAGGGAGGAGCAGCCGTTGATCCTGACTC
  TGGTCTGGAACACTCTGCACATGTCCTGGAGAAAGGCGGGAAGGTGTTCAGTGCCACGCTTGGCCTGGTG
  GACATTGTGAAAGGGACTAACTCCTATTACAAACTGCAACTTCTGGAGTCCGACAAGGAGAGCAGGTATT
  GGATCTTCCGGTCCTGGGGCCGAGTGGGCACAGTTATCGGCAGTAACAAGCTTGAGCAGATGCCGTCCAA
  AGAGGATGCTGTTGAACACTTCATGAAGTTATACGAAGAGAAGACTGGAAATGCCTGGCACTCGAAAAAC
  TTCACAAAGTATCCCAAGAAGTTCTACCCTCTGGAGATTGACTATGGCCAGGACGAAGAGGCAGTAAAGA
  AGCTGGCGGTGAAGCCTGGCACTAAGTCGAAGCTGCCGAAGCCGGTGCAGGAGCTCGTCGGGATGATCTT
  CGACGTGGAGAGCATGAAGAAGGCCTTGGTGGAGTACGAGATTGACCTTCAGAAGATGCCCTTGGGGAAA
  CTGAGCAGAAGGCAGATCCAGGCCGCCTACTCTATCCTCAGTGAGGTCCAGCAGGCAGTGTCCCAAGGCA
  GCAGTGAATCTCAGATCCTGGATCTCTCCAATCGCTTCTACACCCTCATCCCCCATGACTTTGGAATGAA
  GAAGCCCCCACTTCTGAACAACACAGACAGTGTGCAGGCCAAGGTGGAGATGCTGGACAACCTCCTGGAC
  ATCGAGGTTGCCTATAGTCTTCTCAGGGGTGGGTCTGATGACAGCAGTAAGGACCCCATCGATGTCAACT
  ATGAGAAGCTCAAAACTGACATTAAGGTGGTTGACAGAGATTCTGAAGAGGCTGAGGTCATCAGGAAGTA
  TGTGAAGAACACTCACGCGACCACGCACAATGCCTATGACCTGGAAGTGATAGACATCTTTAAGATAGAG
  CGAGAGGGAGAGAGCCAACGCTACAAGCCCTTCAGGCAGCTTCACAACCGGAGACTGCTGTGGCACGGGT
  CCAGGACCACCAACTTCGCAGGCATCCTGTCACAGGGTCTGCGGATAGCCCCACCTGAAGCACCTGTGAC
  AGGCTACATGTTTGGGAAAGGAATCTACTTTGCTGATATGGTGTCCAAAAGTGCGAACTACTGCCACACG
  TCTCAGGGAGACCCGATTGGCTTAATACTGTTGGGAGAAGTTGCCCTTGGAAACATGTACGAACTCAAGC
  ATGCTTCTCACATCAGCAAGTTACCCAAGGGCAAGCACAGTGTCAAAGGTTTGGGCAAAACCGCCCCTGA
  CCCTTCGGCCAGCATCACCCTGGATGGTGTAGAGGTTCCGCTGGGAACAGGGATTCCGTCTGGTGTTAAT
  GACACCTGCCTGCTGTATAACGAGTACATTGTCTACGACATTGCTCAGGTGAATCTGAAGTACCTACTGA
  AACTGAAGTTCAATTTCAAGACCTCCTTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205456 representing NM_013063
  Red=Cloning site Green=Tags(s)
  
  MAEATERLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRQPDTE
  VDGFSELRWDDQQKVKKTAEAGGVAGKGQHGGGGKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQMRLS
  KKMLDPEKPQLGMIDRWYHPTCFVKNRDELGFRPEYSASQLKGFSLLSAEDKEALKKQLPAVKSEGKRKC
  DEVDGIDEVAKKKSKKGKDKESSKLEKALKAQNELVWNIKDELKKACSTNDLKELLIFNQQQVPSGESAI
  LDRVADGMAFGALLPCKECSGQLVFKSDAYYCTGDVTAWTKCMVKTQNPSRKEWVTPKEFREISYLKKLK
  IKKQDRLFPPESSAPAPPAPPVSITSAPTAVNSSAPADKPLSNMKILTLGKLSQNKDEAKAMIEKLGGKL
  TGSANKASLCISTKKEVEKMSKKMEEVKAANVRVVCEDFLQDVSASAKSLQELLSAHSLSSWGAEVKVEP
  GEVVVPKGKSAAPSKKSKGAVKEEGVNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLV
  DIVKGTNSYYKLQLLESDKESRYWIFRSWGRVGTVIGSNKLEQMPSKEDAVEHFMKLYEEKTGNAWHSKN
  FTKYPKKFYPLEIDYGQDEEAVKKLAVKPGTKSKLPKPVQELVGMIFDVESMKKALVEYEIDLQKMPLGK
  LSRRQIQAAYSILSEVQQAVSQGSSESQILDLSNRFYTLIPHDFGMKKPPLLNNTDSVQAKVEMLDNLLD
  IEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEVIRKYVKNTHATTHNAYDLEVIDIFKIE
  REGESQRYKPFRQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHT
  SQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTAPDPSASITLDGVEVPLGTGIPSGVN
  DTCLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013063
• ORF Size: 3042 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013063.1, NM_013063.2, NP_037195.1
• RefSeq Size: 3571
• RefSeq ORF: 3045
• Locus ID: 25591
• MW: 112.7 kDa
• Gene Summary: catalyzes poly (ADP-ribose) protein modification; plays a role in DNA repair and genome stability [RGD, Feb 2006]