Cs (NM_130755) Rat Tagged ORF Clone

CAT#: RR205482

Cs (Myc-DDK-tagged ORF) - Rat citrate synthase (Cs), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205482 representing NM_130755
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACTCACTGCGGCAGCCCGGCTCTTGGGAGCCAAGAACTCATCCTGCCTCGTCCTTGCTGCCC
  GGCACGCCAGTGCTTCTTCCACGAATTTGAAAGACATACTGAGCAATCTGATACCTAAGGAGCAGGCCAG
  AGTTAAGACCTTCAGACAGCAGCATGGGAAGACAGTGGTTGGCCAAATCACTGTGGACATGATGTACGGT
  GGCATGAGAGGCATGAAGGGACTTGTGTATGAGACATCGGTTCTTGATCCTGATGAGGGCATCCGCTTCC
  GAGGCTATAGTATCCCTGAGTGCCAGAAACTGCTACCTAAGGCTAAAGGTGGGGAAGAGCCCCTACCTGA
  GGGCTTATTTTGGCTGCTGGTAACTGGACAGATGCCCACAGAGGAACAGGTCTCTTGGCTCTCACAAGAA
  TGGGCAAAAAGGGCAGCTCTGCCTTCCCATGTGGTCACCATGCTGGACAATTTTCCGACCAATCTGCACC
  CCATGTCTCAGCTCAGTGCAGCCATCACAGCCCTCAACAGTGAAAGCAACTTCGCCCGGGCATATGCAGA
  GGGAATAAACCGAACTAAGTACTGGGAGCTCATCTATGAAGACTGTATGGACCTGATTGCCAAGCTGCCG
  TGCGTTGCAGCAAAGATCTACCGGAATCTGTACCGGGAGGGCAGCAGTATTGGGGCAATTGACTCTAAGC
  TGGACTGGTCCCACAATTTTACCAACATGTTATGCTACACAGAACCTCAGTTCACAGAACTCATGCGTTT
  GTACCTTACCATCCACAGTGACCATGACGGTGGCAATGTAAGTGCCCACACAAGCCACTTGGTGGGCAGC
  GCCCTTTCAGACACTTACCTGTCCTTTGCAGCAGCTATGAATGGGCTGGCAGGGCCTCTGCATGGACTAG
  CAAACCAGGAGGTGCTTGTCTGGCTGACGCAGCTACAGAAGGAAGTCGGCAAAGACGTGTCAGATGAGAA
  GTTACGAGACTACATCTGGAACACACTCAACTCGGGACGGGTTGTCCCAGGATATGGTCATGCAGTCCTA
  AGGAAGACTGATCCACGATATTCCTGTCAGCGAGAGTTTGCTCTGAAACATCTGCCCAAGGATCCCATGT
  TTAAGCTGGTTGCTCAGCTGTACAAGATTGTGCCCAATATCCTCTTAGAGCAAGGAAAGGCTAAGAACCC
  CTGGCCCAACGTAGATGCTCACAGTGGGGTGCTGCTCCAGTACTATGGCATGACGGAGATGAATTACTAC
  ACAGTCCTGTTCGGAGTGTCGAGGGCACTGGGTGTGTTGGCCCAGCTTATCTGGAGCAGAGCCCTAGGCT
  TTCCTCTAGAAAGGCCCAAGTCCATGAGCACGGATGGTCTGATGAAGTTTGTGGACTCTAAGTCAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205482 representing NM_130755
  Red=Cloning site Green=Tags(s)
  
  MALLTAAARLLGAKNSSCLVLAARHASASSTNLKDILSNLIPKEQARVKTFRQQHGKTVVGQITVDMMYG
  GMRGMKGLVYETSVLDPDEGIRFRGYSIPECQKLLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSQE
  WAKRAALPSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGINRTKYWELIYEDCMDLIAKLP
  CVAAKIYRNLYREGSSIGAIDSKLDWSHNFTNMLCYTEPQFTELMRLYLTIHSDHDGGNVSAHTSHLVGS
  ALSDTYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVL
  RKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYY
  TVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTDGLMKFVDSKSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130755
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130755.1, NP_570111.1
• RefSeq Size: 1498
• RefSeq ORF: 1401
• Locus ID: 170587
• MW: 51.9 kDa
• Gene Summary: human homolog is an enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A [RGD, Feb 2006]