Aanat (NM_012818) Rat Tagged ORF Clone

CAT#: RR205489

  • TrueORF®

Aanat (Myc-DDK-tagged ORF) - Rat arylalkylamine N-acetyltransferase (Aanat), (10 ug)


  "NM_012818" in other vectors (3)

Reconstitution Protocol

USD 420.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Aanat
Synonyms AA-NAT; Nat4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205489 representing NM_012818
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAGCATCCACCCCCTGAAACCTGAGGCCCTGCATCTGCCTCTTGGGACCTCAGAGTTCCTAGGCT
GCCAGCGGCGCCACACACTCCCTGCCAGTGAGTTCCGCTGCCTCACGCCGGAGGATGCCACCAGTGCGTT
TGAGATTGAGCGCGAAGCCTTTATCTCAGTCTCGGGTACCTGCCCCCTCCACTTGGATGAGATCCGGCAC
TTCCTCACCCTGTGTCCAGAGCTGTCACTGGGCTGGTTCGAGGAGGGCTGTCTTGTGGCCTTCATCATCG
GTTCACTTTGGGACAAGGAGAGACTTACTCAGGAGTCGCTGACACTACACAGGCCTGGAGGCCGCACGGC
CCACCTGCACGTACTGGCCGTGCACCGAACCTTCCGGCAGCAGGGCAAGGGCTCCGTCCTGCTGTGGCGA
TACCTTCACCACCTGGGGAGTCAGCCGGCGGTGCGCCGGGCTGTGCTCATGTGCGAGAACGCCCTGGTGC
CCTTCTATGAGAAATTTGGTTTCCAGGCCATGGGCCCGTGCGCCATCACCATGGGATCTCTCACCTTCAC
TGAGCTGCAATGTTCCTTACGGTGCCACACCTTCCTGAGGAGGAACAGTGGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205489 representing NM_012818
Red=Cloning site Green=Tags(s)

MLSIHPLKPEALHLPLGTSEFLGCQRRHTLPASEFRCLTPEDATSAFEIEREAFISVSGTCPLHLDEIRH
FLTLCPELSLGWFEEGCLVAFIIGSLWDKERLTQESLTLHRPGGRTAHLHVLAVHRTFRQQGKGSVLLWR
YLHHLGSQPAVRRAVLMCENALVPFYEKFGFQAMGPCAITMGSLTFTELQCSLRCHTFLRRNSGC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012818
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_012818.1, NM_012818.2, NP_036950.1
RefSeq Size 1311
RefSeq ORF 618
Locus ID 25120
MW 23.1 kDa
Gene Summary The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. [provided by RefSeq, Feb 2014]

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