B3galnt1 (NM_001013158) Rat Tagged ORF Clone

CAT#: RR205628

B3galnt1 (Myc-DDK-tagged ORF) - Rat beta-1,3-N-acetylgalactosaminyltransferase 1 (B3galnt1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B3galnt1
• Synonyms: B3galt3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205628 representing NM_001013158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGGCTGTCTTGACTGCCATTCCCAATAGGATGTCACTGAGATCCCTCAAATGGAGCCTCCTGC
  TGCTGTCCCTGCTGAGCTTCCTGGTGATCTGGTACCTCAGCCTTCCCCACTACAATGTGATTGAGCGTGT
  GAACTGGATGTACTTCTATGAGTATGAGCCAATTTACAGACAAGACTTTCAATTCACACTCAGGGAACAT
  TCGAACTGCTCTCAACAAAACCCATTCCTGGTCATCCTGGTGACCTCTCGTCCCTCAGATGTGAAAGCCA
  GACAAGCCATTAGAGTTACTTGGGGCGAGAAAAAAACCTGGTGGGGACACGAGGTTCTTACATTTTTCTT
  ACTTGGCCAGGAGGCTGAAAGGGAAGACAAGGTATTAGCATTGTCCTTGGAGGATGAACATGCTCTCTAT
  GGCGACATTATACGGCAAGACTTTTTAGATACCTACAATAACTTGACCTTGAAAACCATTATGGCTTTCA
  GGTGGGTAATTGAATTTTGCCCCAATGCCAAGTATGTCATGAAAACAGACACCGATGTTTTCATCAACAC
  GGGCAATTTAGTCAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGCTATCCTCTAATT
  GAAAACTATTCCTATAGAGGGTTTTTCCATAAAAACCACATTTCATACCAAGAATACCCGTTCAAGGTGT
  TCCCTCCCTACTGCAGTGGGCTGGGCTACATCATGTCCGGCGACCTGGTGCCGAAGATCTACGAGATGAT
  GGGCCACGTGAAACCCATCAAGTTTGAGGATGTCTATGTCGGCATCTGTCTGAATTTGTTAAAAGTGGAC
  ATTCATATTCCAGAAGACACAAATCTTTTCTTCCTGTTCAGAATACACTTGGACGTGTGTCAGCTGAGAC
  GGGTGATTGCAGCCCATGGCTTTTCCTCCAAGGAGATCATCACCTTCTGGCAGGTCATGCTGAGGAACAC
  CACCTGCCACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205628 representing NM_001013158
  Red=Cloning site Green=Tags(s)
  
  MAPAVLTAIPNRMSLRSLKWSLLLLSLLSFLVIWYLSLPHYNVIERVNWMYFYEYEPIYRQDFQFTLREH
  SNCSQQNPFLVILVTSRPSDVKARQAIRVTWGEKKTWWGHEVLTFFLLGQEAEREDKVLALSLEDEHALY
  GDIIRQDFLDTYNNLTLKTIMAFRWVIEFCPNAKYVMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLI
  ENYSYRGFFHKNHISYQEYPFKVFPPYCSGLGYIMSGDLVPKIYEMMGHVKPIKFEDVYVGICLNLLKVD
  IHIPEDTNLFFLFRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013158
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013158.1, NP_001013176.1
• RefSeq Size: 1928 bp
• RefSeq ORF: 996 bp
• Locus ID: 310508
• Cytogenetics: 2q32
• MW: 39.2 kDa