Neu1 (NM_031522) Rat Tagged ORF Clone

CAT#: RR205676

Neu1 (Myc-DDK-tagged ORF) - Rat sialidase 1 (lysosomal sialidase) (Neu1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neu1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205676 representing NM_031522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGGCAGAGCCAAGCAGACCCCCGGGACCACCGAGCTACTGGACAGGCCGGCGGGGTCAGGGGC
  TCGCAGCGATCTTCCTGCTCCTGGTGTCCGCGGCGGGATCAGAGGCCAGGACAGAGGATGACTTCAGCCT
  GGTGCAGCCGCTGGTGACCATGGAGCAATTGCTGTGGGTGAGCGGGAAGCAGATCGGCTCCGTAGACACT
  TTTCGAATCCCGCTCATCACAGCCACCCCTCGGGGCACGCTCCTGGCCTTCGCTGAGGCTAGGAAAAAGT
  CTGCATCCGATGAGGGGGCCAAGTTCATCGCTATGAGGAGGTCCACAGACCAGGGTAGCACGTGGTCCTC
  TACGGCTTTCATTGTAGACGACGGGGAGGCCTCCGATGGGCTGAACCTGGGGGCTGTGGTGAACGACGTA
  GACACAGGAGTGGTGTTCCTCATCTATACCCTCTGTGCTCACAAGGTCAACTGCCAGGTGGCCTCTACCA
  TGCTGGTTTGGAGTAAGGATGACGGCGTTTCCTGGAGCCCACCGCGGAATCTCTCCGTGGATATTGGCAC
  AGAGATGTTTGCCCCTGGACCCGGCTCTGGCATTCAGAAACAGCGGGAGCCTTGGAAGGGCCGGCTCATT
  GTGTGTGGACACGGGACGCTGGAGCGAGATGGGGTCTTCTGTCTCCTCAGTGATGACCACGGTGCCTCCT
  GGCACTATGGGACTGGAGTGAGCGGCATTCCCTTCGGCCAGCCCAAACACGATCACGATTTCAACCCCGA
  CGAGTGCCAGCCCTACGAGCTTCCAGACGGCTCAGTCATCATCAACGCCCGGAACCAGAATAACTACCAC
  TGCCGCTGCAGGATCGTCCTCCGCAGCTATGACGCCTGTGACACCCTCAGGCCCCGGGATGTGACCTTCG
  ACCCTGAGCTCGTGGACCCTGTGGTAGCTGCAGGAGCGCTAGCCACCAGCTCCGGCATTGTCTTCTTCTC
  CAATCCAGCCCATCCCGAGTACCGAGTGAACCTGACCCTGCGCTGGAGCTTCAGCAATGGCACATCCTGG
  CAGAAGGAGAGGGTCCAGTTGTGGCCGGGACCCAGCGGCTACTCATCCCTGACAGCCCTGGAAAACAGCA
  CGGACGGGAAGAAGCAGCCCCCGCAGCTGTTCGTTCTGTACGAGAAAGGACTGAACCGGTACACGGAGAG
  CATCTCCATGGTCAAAATCAGCGTCTACGGCACGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205676 representing NM_031522
  Red=Cloning site Green=Tags(s)
  
  MVGAEPSRPPGPPSYWTGRRGQGLAAIFLLLVSAAGSEARTEDDFSLVQPLVTMEQLLWVSGKQIGSVDT
  FRIPLITATPRGTLLAFAEARKKSASDEGAKFIAMRRSTDQGSTWSSTAFIVDDGEASDGLNLGAVVNDV
  DTGVVFLIYTLCAHKVNCQVASTMLVWSKDDGVSWSPPRNLSVDIGTEMFAPGPGSGIQKQREPWKGRLI
  VCGHGTLERDGVFCLLSDDHGASWHYGTGVSGIPFGQPKHDHDFNPDECQPYELPDGSVIINARNQNNYH
  CRCRIVLRSYDACDTLRPRDVTFDPELVDPVVAAGALATSSGIVFFSNPAHPEYRVNLTLRWSFSNGTSW
  QKERVQLWPGPSGYSSLTALENSTDGKKQPPQLFVLYEKGLNRYTESISMVKISVYGTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031522
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031522.1, NM_031522.2, NM_031522.3, NP_113710.2
• RefSeq Size: 1931
• RefSeq ORF: 1230
• Locus ID: 24591
• MW: 44.6 kDa
• Gene Summary: enzyme that cleaves sialic acid from glycoproteins and glycolipids [RGD, Feb 2006]