Pigw (NM_194461) Rat Tagged ORF Clone

CAT#: RR205734

Pigw (Myc-DDK-tagged ORF) - Rat phosphatidylinositol glycan anchor biosynthesis, class W (Pigw), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pigw
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205734 representing NM_194461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAAAAGCAGCTGAAGGAAGCTTTTGTCAGTAACCTCAGTGGGACCAGTGTTCTGGAAGTGACCC
  AGGGCCTGTGCTTTCCTGCGTTCTGTATCCTGTGTAGAGGCCTTCTGATCATTTTCTCACAGCATTTGTG
  CTCTTTCTTGCACACCTGGACCACACAATTCTTCATGGACTTTGTTGTCTTGATAGTCCCCCTAGTGATC
  ACTCTGACTGTATTGTCTTCCTTTATCCTCCTTGAGAATCTCACTGTGATTCTCTGTGGGGCGTGGCTGT
  TGTATCAGATATACCACAGGAGGACTTGCTATGCCAAAGTACCTGTCCAGAAAGTCTTTGCAAGCTTCTT
  GAAAATCAGCCTAGAATCAGAGTACAATCCAGCCATCACCTGTTACCGGGTCATTAACAGTGTATTTACT
  GCTATTGCCATTTTGGCTGTGGATTTCCCACTTTTCCCTAGAAGATTTGCCAAAACTGAACTTTATGGGA
  CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTATTTTTGGGGCTGCAATGGTTTGCCCAGAGGTTAGAAG
  GAAATATACAGAAGGGTCTAGATTTAATCATCTTAGAAAATCGCTGTATTCTGTCTGGCCATTAGTCTTC
  TTAGGAATGGGAAGGTTAGTCATTATAAAATCCATAGGCTACCAGGAACATTCAACTGAGTATGGAGTTC
  ACTGGAATTTTTTCTTTACCATCATAGTTGTGAAATTAATAACATCACTGCTTTTGATTATTTTTCCTTT
  AAATAAGTCATGGATTGTGGCCATCAGCATTACTGTGTTGTACCAGCTTGCCCTTGACTTTACTCCACTC
  AAGGGGATAATTTTGTATGGCACTGATGGCAGGGGCACAAGAGTTGGCTTATTAAATGCTAACCGAGAGG
  GAATAATTTCCACCTTGGGGTATGTGGCAATATACATGGCTGGTGTACAAACAGGATTTTATGTGTTCAA
  GAGAAGAGCACAGGTCAGAGACTGGATAAAAGCTACTTGTTGGGTATTCTCGGTGGCTGTTGGCTTCTTC
  ATTTCTCTTAATATAGTGCAAGTAAACGTAGAAGCAGTATCTCGAAGAATGGCCAATTTAGCCTTTTGTC
  TGTGGGTGGTTGCCTCCAGCCTGATGCTGCTTAGTTGTCTGTTACTGAGTGGTATCGTTTTGAGTTTTGC
  CAAATTTCTAATTAAAGGGGCTCTAGTTCCATGTTCTTGGAAACTTATACAGTCTGCTACTACAAATAGA
  CAGTCTGAATCTCTAATCGTAGAAGCTGAAAAGAACAAACCCAGTTTTTGTTTGATCACAGCTCTGAACA
  GAAACCAGTTGTTTTTTTTCTTACTGTCAAATGTAGCAACTGGTCTGATTAACCTGACGGTGGATACATT
  ACACACTGGTGCCTTTTGGACCTTAGCTGTGCTTAGTATTTATATGTTTGCCAACTGCTTAGTTATATAT
  GTACTTGACTTACAAGGTAAAACCATAAAGTTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205734 representing NM_194461
  Red=Cloning site Green=Tags(s)
  
  MSQKQLKEAFVSNLSGTSVLEVTQGLCFPAFCILCRGLLIIFSQHLCSFLHTWTTQFFMDFVVLIVPLVI
  TLTVLSSFILLENLTVILCGAWLLYQIYHRRTCYAKVPVQKVFASFLKISLESEYNPAITCYRVINSVFT
  AIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFIFGAAMVCPEVRRKYTEGSRFNHLRKSLYSVWPLVF
  LGMGRLVIIKSIGYQEHSTEYGVHWNFFFTIIVVKLITSLLLIIFPLNKSWIVAISITVLYQLALDFTPL
  KGIILYGTDGRGTRVGLLNANREGIISTLGYVAIYMAGVQTGFYVFKRRAQVRDWIKATCWVFSVAVGFF
  ISLNIVQVNVEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIVLSFAKFLIKGALVPCSWKLIQSATTNR
  QSESLIVEAEKNKPSFCLITALNRNQLFFFLLSNVATGLINLTVDTLHTGAFWTLAVLSIYMFANCLVIY
  VLDLQGKTIKFW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194461
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194461.1, NP_919443.1
• RefSeq Size: 1509
• RefSeq ORF: 1509
• Locus ID: 378774
• MW: 56.4 kDa
• Gene Summary: inositol acetyltransferase enzyme [RGD, Feb 2006]