Pde1b (NM_022710) Rat Tagged ORF Clone

CAT#: RR205735

Pde1b (Myc-DDK-tagged ORF) - Rat phosphodiesterase 1B, calmodulin-dependent (Pde1b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde1b
• Synonyms: Aop2; Pde1b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205735 representing NM_022710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTCCCCCCGCAGTCCTCCAGAGATGCTGGAGTCGGATTGCCCGTCACCCCTGGAGCTGAAGT
  CAGCCCCCAGCAAGAAGATGTGGATTAAGCTACGGTCTCTGCTGCGCTACATGGTGAAGCAGTTGGAGAA
  TGGGGAGGTTAACATCGAGGAGCTGAAGAAAAACCTGGAATACACAGCTTCCCTGCTGGAAGCTGTCTAC
  ATTGATGAGACAAGGCAAATCTTGGACACTGAAGATGAGCTTCGGGAACTTCGGTCAGATGCCGTGCCTT
  CAGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGACCCGAGCCAAAGGTCGCAGGGCAGAAGA
  GAAACCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAGCGGATGTTCCGGAGA
  ACGTATACCGCTGTGGGTCCCACGTATTCTACTGCAGTCCACAACTGTCTCAAGAACCTGGACGTCTGGT
  GCTTCGATGTCTTCTCTTTGAACCGGGCAGCTGATGACCACGCTCTGAGGACCATTGTTTTTGAGTTGCT
  GACTCGGCATAGCCTCATCAGCCGCTTCAAGATTCCCACAGTGTTTCTGATGAGTTTTCTGGAGGCCTTA
  GAGACAGGCTACGGGAAATATAAGAACCCTTACCACAACCAGATCCATGCCGCTGACGTGACCCAGACTG
  TCCATTGCTTCCTGCTCCGCACAGGCATGGTGCACTGCCTGTCAGAGATTGAGGTCTTGGCCATCATCTT
  CGCGGCAGCCATCCATGACTATGAGCACACAGGCACAACCAACAGCTTCCACATCCAGACCAAGTCAGAA
  TGCGCCATCCTGTACAATGACCGATCGGTGCTGGAGAATCACCACATCAGCTCTGTTTTCCGAATGATGC
  AGGATGATGAGATGAACATTTTTATCAATCTCACCAAGGATGAATTCGTAGAGCTGCGGGCCCTGGTCAT
  TGAGATGGTGTTAGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACTATGAAGACAGCCCTGCAG
  CAGCTTGAAAGGATTGACAAATCCAAGGCCCTGTCTCTTCTGCTTCATGCTGCTGACATCAGCCACCCAA
  CCAAGCAGTGGTCAGTCCACAGCCGCTGGACCAAGGCCCTAATGGAAGAGTTCTTCCGCCAGGGTGACAA
  GGAGGCAGAGCTGGGCTTGCCCTTCTCTCCACTCTGTGACCGCACCTCCACATTGGTGGCCCAGTCCCAG
  ATAGGTTTCATTGACTTCATTGTGGAGCCCACCTTCTCTGTGCTGACTGATGTGGCAGAAAAGAGTGTCC
  AGCCCTTGACAGATGATGATTCCAAGTCTAAAAGTCAGCCCAGCTTCCAGTGGCGCCAGCCTTCTTTAGA
  TGTGGACGTAGGAGACCCCAACCCTGATGTGGTCAGTTTCCGCTCCACCTGGACCAAGTACATTCAAGAG
  AATAAACAGAAGTGGAAGGAACGGGCAGCGAGTGGCATCACCAACCAGATGTCCATTGATGAACTGTCCC
  CCTGTGAGGAAGAAGCCCCATCCTCCCCTGCAGAAGATGAGCACAACCAGAATGGGAATCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205735 representing NM_022710
  Red=Cloning site Green=Tags(s)
  
  MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEVNIEELKKNLEYTASLLEAVY
  IDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRR
  TYTAVGPTYSTAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEAL
  ETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSE
  CAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ
  QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQ
  IGFIDFIVEPTFSVLTDVAEKSVQPLTDDDSKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQE
  NKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022710
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022710.1, NP_073201.1
• RefSeq Size: 1831
• RefSeq ORF: 1608
• Locus ID: 29691
• MW: 61.3 kDa
• Gene Summary: phosphodiesterase isozyme that catalyzes the calmodulin dependent hydrolysis of cGMP; expressed in brain [RGD, Feb 2006]