Lif (NM_022196) Rat Tagged ORF Clone

CAT#: RR205959

Lif (Myc-DDK-tagged ORF) - Rat leukemia inhibitory factor (cholinergic differentiation factor) (Lif), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lif
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205959 representing NM_022196
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTCTTGGCCACAGGGATTGTGCCCCTACTGCTCATTCTGCACTGGAAACACGGGGCAGGGAGCC
  CCCTTCCCATCACCCCTGTAAATGCCACCTGCGCCATACGCCACCCGTGTCACGGCAACCTCATGAACCA
  GATCAAGAGTCAACTGGCTCAACTCAACGGCAGTGCCAATGCCCTCTTTATTTCCTATTACACAGCTCAA
  GGGGAACCATTTCCCAACAACGTGGATAAGCTATGTGCGCCCAACATGACGGATTTCCCACCTTTCCATG
  CCAATGGGACAGAGAAGACCAAGTTGGTCGAGCTGTATCGGATGGTCGCGTACCTGGGAGCCTCCCTGAC
  CAACATCACCTGGGATCAGAAAAACCTCAACCCCACTGCCGTGAGCCTCCAGATCAAACTCAATGCGACT
  ACAGACGTCATGAGGGGGCTCCTTAGCAACGTGCTTTGCCGTCTGTGCAACAAGTACCATGTGGGCCATG
  TGGATGTGCCCTGTGTCCCCGACAACTCTAGCAAAGAAGCCTTCCAAAGGAAGAAGTTGGGCTGCCAGCT
  CCTGGGGACATACAAGCAAGTCATTAGTGCGGTGGTCCAGGCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205959 representing NM_022196
  Red=Cloning site Green=Tags(s)
  
  MKVLATGIVPLLLILHWKHGAGSPLPITPVNATCAIRHPCHGNLMNQIKSQLAQLNGSANALFISYYTAQ
  GEPFPNNVDKLCAPNMTDFPPFHANGTEKTKLVELYRMVAYLGASLTNITWDQKNLNPTAVSLQIKLNAT
  TDVMRGLLSNVLCRLCNKYHVGHVDVPCVPDNSSKEAFQRKKLGCQLLGTYKQVISAVVQAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022196
• ORF Size: 606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022196.1, NM_022196.2, NP_071532.2
• RefSeq Size: 609
• RefSeq ORF: 609
• Locus ID: 60584
• MW: 22.1 kDa
• Gene Summary: a secreted cytokine; involved in embryonic stem cell and myeloid cell growth and differentiation and stimulation of bone remodeling [RGD, Feb 2006]