Chpt1 (NM_001007750) Rat Tagged ORF Clone

CAT#: RR206162

Chpt1 (Myc-DDK-tagged ORF) - Rat choline phosphotransferase 1 (Chpt1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chpt1
• Synonyms: MGC95241
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206162 representing NM_001007750
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGCGCCGGGGCCAGGCCGGCGCCGCGCTGGGTGAAGGCGCTAGGTGAGCCGCTCAGCGCCG
  CGCAGCTGCGGCGGCTGGAGGACCACCGCTACAGCGCGGCGGGAGAGTCGCTGTTCGAGCCGCCGCTGCA
  GCTTTTCTGGACCTGGCTGCTCCAATGGATCCCGCTCTGGATAGCCCCCAACACCATCACCCTCTTCGGC
  CTCGCCATCAACCTGTTCACCACACTAGTGCTCATCTTCTACTGCCCTACGGTCACGGAGGAGGCACCAT
  ACTGGACATACCTTTTATGTGCCCTGGGACTCTTTATCTACCAGTCACTGGATGCCATTGACGGAAAACA
  AGCCAGAAGGACAAATTCCTGCTCTCCTTTAGGGGAACTGTTTGATCATGGTTGTGACTCTCTTTCCACA
  GTATTTATGGCTATCGGTGCTTCAATTGCTGTTCGTCTAGGAACACATCCTGACTGGTTGTTTTTCTGCT
  CTTTCGTTGGGATGTTCATGTTTTATTGTGCTCATTGGCAGACTTACGTCTCAGGAGTGTTGCGATTTGG
  AAGGGTGGATGTAACTGAGATCCAGGTAGCTTTAGTGATTGTCTTTCTGTTATCTACATTCGGAGGAGCA
  ATGATGTGGGACTATACGATACCTATTCTAGAAATAAAACTGAAGATCCTTCCGGTTCTTGGAGTTGTAG
  GTGGATTAATATTTTCCTGTTCAAACTATTTCCATGTGATCCTCCATGGTGGTGTTGGCAAGAATGGATC
  TACTATTGCGGGCACCAGTGTCTTGTCACCTGGACTCCACATAGGATTAATTATTATATTGGCAATAATG
  ATCTATAAAAAATCTGCAACAAATGTGTTTGAAAAACACCCTTGCCTTTATACATTAATGTTTGGATGTG
  TCTTTGCTAAAGTTGCACAAAAATTGGTGATAGCTCACATGACAAAAAGTGAACTATATCTTCAAGACAC
  TGTTTTTATTGGGCCAGGTCTTTTATTTTTAGACCAATACTTTAATAATTTTATAGATGAATATGTTGTT
  CTATGGATAGCAATGGTCATTACTTCATTTGATATGATGATATACTTTAGTTCTTTGTGCCTGCAAATTT
  CAAGACACCTTCATCTAAGTATCTTCAAGACTTCATATCAACAAGCACCTGAACAGGTTCACAAGCATAT
  TGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206162 representing NM_001007750
  Red=Cloning site Green=Tags(s)
  
  MAAGAGARPAPRWVKALGEPLSAAQLRRLEDHRYSAAGESLFEPPLQLFWTWLLQWIPLWIAPNTITLFG
  LAINLFTTLVLIFYCPTVTEEAPYWTYLLCALGLFIYQSLDAIDGKQARRTNSCSPLGELFDHGCDSLST
  VFMAIGASIAVRLGTHPDWLFFCSFVGMFMFYCAHWQTYVSGVLRFGRVDVTEIQVALVIVFLLSTFGGA
  MMWDYTIPILEIKLKILPVLGVVGGLIFSCSNYFHVILHGGVGKNGSTIAGTSVLSPGLHIGLIIILAIM
  IYKKSATNVFEKHPCLYTLMFGCVFAKVAQKLVIAHMTKSELYLQDTVFIGPGLLFLDQYFNNFIDEYVV
  LWIAMVITSFDMMIYFSSLCLQISRHLHLSIFKTSYQQAPEQVHKHID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007750
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007750.1, NP_001007751.1
• RefSeq Size: 1555 bp
• RefSeq ORF: 1197 bp
• Locus ID: 362866
• Cytogenetics: 7q13
• MW: 44.6 kDa