Prkg2 (NM_013012) Rat Tagged ORF Clone

CAT#: RR206197

Prkg2 (Myc-DDK-tagged ORF) - Rat protein kinase, cGMP-dependent, type II (Prkg2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkg2
• Synonyms: cGKII; GDPKII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206197 representing NM_013012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATGGTTCAGTGAAACCCAAGCATTCGAAGCACCCAGACGGCCAGTCAGGGAACCTCAGCAATG
  AAGCCCTGCGGAGCAAAGTGGCAGAGCTGGAGCGCGAGGTGAAGAGGAAGGATGCAGAACTTCAGGAGCG
  CGAGTACCACCTGAAGGAGCTTCGGGAGCAGCTAGCTAAGCAGACCGTGGCCATTGCGGAGCTCACCGAG
  GAGCTGCAGAGCAAGTGCATCCAGCTGAACAAGCTGCAGGATGTTATCCACGTGCAGGGAGGAAGCCCAC
  TGCAGGCCTCTCCCGACAAAGTGCCTCTGGATGTTCACCGCAAGACCTCAGGCTTGGTCTCCCTCCACAG
  CAGGAGGGGGGCGAAGGCTGGAGTGTCTGCAGAGCCCACAAGCCGGACCTATGACCTCAACAAGCCCCCT
  GAGTTCTCCTTTGAGAAAGCGAGGGTCAGGAAGGATTCCAGTGAGAAAAAACTTATCACAGACGCCCTGA
  ATAAGAACCAGTTTCTGAAGAGACTGGATCCGCAGCAGATCAAAGACATGGTGGAATGCATGTACGGGAG
  AAACTATCAACAGGGCAGTTACATCGTTAAGCAAGGGGAACCTGGAAACCACATCTTTGTGCTGGCCGAG
  GGTAGACTGGAGGTGTTCCAAGGAGAGAAATTGCTGTCGTCCATCCCAATGTGGACCACGTTTGGGGAGC
  TGGCTATTTTGTATAATTGTACGCGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACTTGGGCTCT
  CGATAGAGAGGTTTTCCAGAACATTATGAGGAGAACAGCGCAAGCCAGGGATGAGGAATACAGAAACTTC
  CTCAGAAGTGTCTCCTTGTTGAAGAATTTACCTGAAGACAAACTAACCAAGATTATTGACTGCTTGGAAG
  TGGAATACTATGACAAAGGAGATTATATCATCAGAGAAGGAGAGGAAGGGAGCACCTTTTTCATCTTGGC
  AAAAGGAAAGGTGAAAGTCACACAGAGCACAGAGGGCCATGATCAGCCACAGTTGATCAAAACACTGCAG
  AAAGGAGAGTACTTTGGAGAAAAGGCTCTGATCAGTGATGATGTCAGATCGGCCAATATAATCGCGGAAG
  AAAATGATGTCGCATGCCTCGTGATCGACCGAGAAACATTCAACCAAACTGTCGGGACATTTGATGAACT
  CCAAAAATACCTTGAGGGGTATGTGGCCACCCTGAACCGTGACGATGAAAAGAGACACGCAAAGCGCTCC
  ATGTCTAGCTGGAAGTTGTCCAAAGCCCTTTCTCTGGAGATGATTCAGCTGAAAGAGAAGGTGGCCAGAT
  TCTCCTCAACCTCCCCATTCCAGAACCTTGAGATTATCGCAACACTGGGCGTTGGTGGGTTCGGAAGAGT
  GGAGCTTGTTAAAGTTAAAAATGAGAACATCGCTTTTGCCATGAAGTGTATAAGGAAGAAGCACATTGTT
  GACACTAAACAGCAGGAACATGTCTACTCCGAGAAGAGGATCCTGGAGGAGCTGTGTTCTCCCTTCATCG
  TGAAATTGTATCGTACTTTCAAGGACAATAAATACGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGA
  GCTCTGGAGTATATTAAGGGACAGAGGCAGCTTCGATGAACCCACCTCCAAATTCTGTGTGGCCTGCGTG
  ACAGAAGCATTCGATTATCTACACCGACTAGGCATTATCTACAGAGACCTGAAGCCAGAGAACTTAATTC
  TGGATGCCGATGGCTACCTTAAGTTGGTTGACTTTGGATTCGCTAAGAAGATTGGCTCTGGGCAGAAAAC
  GTGGACATTCTGTGGGACTCCAGAGTATGTGGCCCCCGAGGTCATTCTTAACAAAGGACATGACTTCAGT
  GTGGATTTCTGGTCCCTGGGGATTCTGGTCTATGAGCTTCTAACGGGCAACCCTCCCTTTTCTGGGATAG
  ACCAAATGATGACCTACAATTTGATTCTCAAAGGGATCGAGAAAATGGATTTTCCTAGAAAGATAACAAG
  GCGACCCGAGGATTTGATCCGGAGGCTTTGCAGGCAAAACCCAACAGAAAGGCTGGGGAACCTGAAGAAT
  GGGATCAACGACATTAAGAAACACAGGTGGTTAAACGGTTTCAATTGGGAGGGGTTGAAAGCACGGAGCC
  TTCCCTCACCTTTACGAAGAGAGCTCAGTGGCCCCATAGATCACAGCTACTTTGACAAGTATCCTCCTGA
  AAAGGGGGTTCCTCCGGATGAAATGTCAGGCTGGGATAAGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206197 representing NM_013012
  Red=Cloning site Green=Tags(s)
  
  MGNGSVKPKHSKHPDGQSGNLSNEALRSKVAELEREVKRKDAELQEREYHLKELREQLAKQTVAIAELTE
  ELQSKCIQLNKLQDVIHVQGGSPLQASPDKVPLDVHRKTSGLVSLHSRRGAKAGVSAEPTSRTYDLNKPP
  EFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIVKQGEPGNHIFVLAE
  GRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEEYRNF
  LRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQ
  KGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKRS
  MSSWKLSKALSLEMIQLKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENIAFAMKCIRKKHIV
  DTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACV
  TEAFDYLHRLGIIYRDLKPENLILDADGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFS
  VDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKN
  GINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFDKYPPEKGVPPDEMSGWDKDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013012
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013012.1, NP_037144.1
• RefSeq Size: 2990
• RefSeq ORF: 2289
• Locus ID: 25523
• MW: 87.2 kDa
• Gene Summary: may regulate renin release and renal ion transport [RGD, Feb 2006]