Pde5a (NM_133584) Rat Tagged ORF Clone

CAT#: RR206532

Pde5a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 5A, cGMP-specific (Pde5a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde5a
• Synonyms: PDE5A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206532 representing NM_133584
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCTTTGGAGACAAAACGAGAGACATGGTCAATGCATGGTTTTCAGAGAGAGTTCACAACATTC
  CTGTGTGCAAGGAAGGCATCCGGGCCCACACCGAGTCCTGCTCTTGCTCTCTGCCTCAAAGTCCTCATGC
  AGATAATACCACCCCTGGAGCACCAGCCAGAAAAATCTCTGCCTCTGAATTTGACCGACCTCTTAGACCC
  ATTGTTGTCAAGGATTCTGAGGGAACCGTGAGCTTTCTCTCTGACTCAGGAAAAAAGGAACAGATGCCAC
  TAACCTCTCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCAAGACTCTTGGAACTAGTAAAAGATAT
  TTCCAGTCATTTGGATGTCACAGCCTTGTGTCACAAAATTTTCTTGCATATCCATGGACTTATCTCTGCT
  GATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGACAAATTTCTCGTCAGTCGCCTCTTTG
  ATGTTGCTGAAGGTTCAACACTGGAGGAAGCTTCAAATAACTGTATCCGTTTAGAATGGAACAAAGGCAT
  TGTGGGACATGTGGCAGCTTTTGGAGAGCCCTTGAACATCAAAGATGCCTATGAGGATCCCCGGTTCAAT
  GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCTTTGTATGCCAATTAAGAACCACAGGG
  AAGAGGTCGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTCACTGAAAAAGA
  TGAAAAGGACTTTGCTGCCTATCTGGCATTTTGTGGCATTGTTCTTCACAACGCCCAGCTCTATGAAACG
  TCACTGCTGGAGAACAAAAGAAATCAGGTATTACTTGACCTTGCTAGCTTAATTTTCGAGGAGCAGCAGT
  CATTGGAAGTAATTCTGAAGAAAATAGCCGCCACTATTATCTCCTTCATGCAGGTGCAGAAGTGCACCAT
  CTTCATCGTGGATGAAGACTGCCCTGACTCGTTCTCTCGTGTGTTTCAAATGGAGTGGGAGGAAGTAGGG
  AAATCCTCCGAGCCTTTGACAAGGGAGCACGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA
  AAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGACAACAGATTTCCCTGGACAAATGAAAA
  CATGGGCCATATAAACACGCACTGCATCAGAAGTTTGCTTTGCACGCCTATAAAAAATGGGAAGAAGAAC
  AAAGTCATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAAGACTGGCAAGATTAAGGCTTTCAACC
  AAAACGATGAACAATTCCTGGAGGCTTTTGTCATCTTCTGTGGCTTGGGGATCCAGAACACACAGATGTA
  CGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACGTTAGAGGTCCTGTCTTATCACGCATCAGCG
  GCGGAGGAAGAAACAAGGGAGCTCCAGGCTCTAGCGGCTGCTGTGGTACCGTCTGCCCAAACCCTTAAAA
  TTACCGACTTCAGCTTCAGTGACTTCGAGCTGTCTGATCTGGAAACAGCGCTGTGTACAATTCGGATGTT
  CACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACACGAGGTTCTTTGCCGATGGATTTTGAGTGTC
  AAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCACGCCTTTAACACGGCACAGTGCATGT
  TTGCTGCTCTGAAAGCAGGCAAGATTCAGAACAAGTTGACGGATCTGGAGACGCTTGCATTGCTGATTGC
  CGCTTTAAGCCATGATTTGGACCATCGTGGTGTGAACAACTCATACATACAGCGGAGCGAGCACCCCCTG
  GCCCAGCTGTACTGCCACTCCACCATGGAGCACCATCACTTCGACCAGTGCTTGATGGTTCTAAACAGCC
  CAGGCAACCAGATCCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACATTGAAAATAATCAAGCAAGC
  AATTTTAGCCACTGACCTAGCACTGTACATTAAGAGACGAGGAGAATTTTTTGAACTTATAAGAAAAAAT
  GAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTGGCAATGCTGATGACTGCCTGTGATCTCT
  CCGCCATTACTAAGCCCTGGCCTATTCAACAACGGATAGCAGAACTCGTGGCAGCCGAATTCTTTGATCA
  AGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCCGCTGATCTAATGAACAGGGAGAAGAAAAATAAA
  ATCCCAAGTATGCAAGTTGGGTTCATAGACGCCATCTGCTTGCAGCTGTATGAGGCCCTGACCCACGTCT
  CAGAAGACTGTTTGCCCCTGCTGGATGGCTGCAGGAAGAACAGGCAGAAATGGCAAGCCCTCGCAGACCA
  GCAGGAGAAGACGCTCCTTAATGGGGAGAGCGGCCAGGCCAAGCGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206532 representing NM_133584
  Red=Cloning site Green=Tags(s)
  
  MLPFGDKTRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLPQSPHADNTTPGAPARKISASEFDRPLRP
  IVVKDSEGTVSFLSDSGKKEQMPLTSPRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISA
  DRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFN
  AEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYET
  SLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCPDSFSRVFQMEWEEVG
  KSSEPLTREHDANKINYMYAQYVKNTMEPLNIPDVTKDNRFPWTNENMGHINTHCIRSLLCTPIKNGKKN
  KVIGVCQLVNKMEEKTGKIKAFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASA
  AEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSV
  KKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPL
  AQLYCHSTMEHHHFDQCLMVLNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKN
  EFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAELVAAEFFDQGDRERKELNMEPADLMNREKKNK
  IPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNRQKWQALADQQEKTLLNGESGQAKRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133584
• ORF Size: 2499 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133584.1, NP_598268.1
• RefSeq Size: 3397
• RefSeq ORF: 2502
• Locus ID: 171115
• MW: 94.6 kDa
• Gene Summary: catalyzes the hydrolysis of cGMP to 5'-GMP; may regulate vascular relaxation; may be involved in establishment of cerebellar neural networks [RGD, Feb 2006]