Cnot6 (NM_001013856) Rat Tagged ORF Clone

CAT#: RR206851

Cnot6 (Myc-DDK-tagged ORF) - Rat CCR4-NOT transcription complex, subunit 6 (Cnot6), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot6
• Synonyms: CCR4; RGD1310783
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206851 representing NM_001013856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG
  CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT
  GTGGTCACTAACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT
  GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG
  GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG
  AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT
  CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGGTACTGCAAAAAGAA
  TTTCAACAGAACAACCACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGC
  CTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGT
  CCATCATGGGCACTAAATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTG
  ATATTATAAGTCTTCAGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACG
  TGGCTATAATGGATTCTTTAGTCCTAAGTCTCGAGCTAGGACTATGTCTGAACAGGAAAGAAAGCATGTT
  GATGGCTGTGCAATATTCTTCAAGACTGAAAAATTTACTTTGGTTCAGAAACACACGGTTGAATTTAATC
  AGCTAGCAATGGCGAATTCAGAAGGGTCTGAAGCTATGTTGAACAGAGTCATGACAAAGGATAACATTGG
  AGTTGCCGTACTGCTAGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACA
  GAAAAACAACTTATTCTTGTGGCTAATGCTCATATGCATTGGGACCCTGAATACTCAGACGTGAAGTTGG
  TGCAAACTATGATGTTCCTTTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAG
  TGTATTGGGAGAATGTGGAACTATTCCACTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGT
  GTTGTAGAGTATTTGAGCACTGGTGGGGTAGAAACAAATCATAAAGACTTTAAGGAACTGAGATACAATG
  AAAGTCTTACAAACTTCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGAATCACACATGGTTTCAA
  GTTAAAGAGTGCCTATGAGAATGGCCTGATGCCTTACACGAATTACACATTTGATTTCAAGGGTATAATT
  GACTACATCTTCTATTCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGACCACCATTGGC
  TAGTTGAGAATAACATCAGCGGCTGCCCACATCCACTTATTCCCTCCGACCACTTCTCACTTTTTGCACA
  ATTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206851 representing NM_001013856
  Red=Cloning site Green=Tags(s)
  
  MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSSSLWSLTHLTALHLSDNSLSCIPSDI
  AKLHNLVYLDLSHNQIQSLPAELGNMVSLRELHLNYNQLRVLPFELGKLFQLQTLSLKGNPLTQDILNLC
  LEPDGTRRLLNYLLDNLSGTAKRISTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYC
  PSWALNWDYRKKAIIQEILSCNADIISLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHV
  DGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKELIEMSSGKPHLGT
  EKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRSLKSSVLGECGTIPLVLCADLNSLPDSG
  VVEYLSTGGVETNHKDFKELRYNESLTNFSCNGKNGMTNGRITHGFKLKSAYENGLMPYTNYTFDFKGII
  DYIFYSKPQLNTLAILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013856
• ORF Size: 1671 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013856.1, NP_001013878.1
• RefSeq Size: 2213 bp
• RefSeq ORF: 1674 bp
• Locus ID: 287249
• Cytogenetics: 10q21
• MW: 63.3 kDa