Abcb10 (NM_001012166) Rat Tagged ORF Clone

CAT#: RR206893

Abcb10 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb10
• Synonyms: MGC109142
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206893 representing NM_001012166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCCCTCCTGCCAGGGCGCTGCTGCTGTTTCCGCGCCGGGGCCCTGCTGTGCGAGCTTGGGCCC
  CGGCCGTCTCCTCTCGGACATGGTTGGCTTCTGAGTGGACTCCGCTCGTACGCGCGTGGACCTCTCTGAT
  CCACGAGCCGAGCCCGGGCCTCCGCTTTCCCGTGCCCCTTTCGGGGCTGCCTGGCGGCGTGGGACGGTGG
  GCCACCTCCTCGGGGATCCGCAGGTGTTGGGTGCTGGCAGGACCCCGCGCCGCGCATCCCCTGTTCGCCA
  GGCTCCAGGGAGCAGCGGCCACCGGTGTGCGAGACCTTGGGAACGACTCGCAGCGGCGGCCCGCGACGAC
  CGGGCGCTCAGAAGTGTGGAAGCTCCTGGGGTTGGTGCGCCCGGAGCGCGGGAGACTGTCAGCTGCGGTT
  GGCTTTCTTGCCGTGTCCAGTGTCATCACCATGTCGGCCCCCTTCTTCCTGGGGAAGATCATTGATGTCA
  TCTACACCAACCCAACCGAGGACTTTGGCGACAGCCTGACTCGCCTCTGTGCTGGGCTCACCTGTGTGTT
  CCTGTGTGGCGCTGCCGCGAACAGCATCCGTGTCTACCTCATGCAGTCTTCAGGTCAGAGCATTGTGAAC
  AGGCTGCGCACCTCACTCTTCTCCTCCATTCTAAGGCAAGAGGTTGCCTTCTTTGACAAGACCCGCACGG
  GCGAGTTGATTAACCGCCTCTCTTCGGATACCGCACTCCTGGGCCACTCCGTGACCGAGAATCTCTCTGA
  CGGGCTGAGGGCTGTGGCCCAGGCCTCGGTTGGTGTTGGCATGATGTTCTTTGTGTCACCAAGTCTGGCC
  ACCTTTGTTCTGAGTGTGGTGCCACCTATCTCTGTCCTCGCTGTGATTTACGGGCGATATCTACGGAAAC
  TGTCCAAAGCCACACAGGACTCTTTGGCAGAAGCCACACAGCTAGCTGAGGAGCGGATTGGAAACATAAG
  AACCATCCGAGCTTTTGGGAAGGAGATGACAGAAGTGGAGAAGTACACCAGCCGAGTGGACCAGTTGCTG
  CAGCTGGCACGGAAGGAGGCCTTGGCACGAGCCGGCTTCTTTGGAGCGGCTGGGCTCTCGGGAAACTTGA
  TTGTGCTGTCTGTCCTGTACAAAGGAGGCCTGTTGCTGGGCAGCACCCACATGACAGTGGGCGAGCTCTC
  TTCCTTCCTCATGTACGCTTTCTGGGTCGGACTGAGCATCGGAGGTCTGAGTTCATTCTACTCCCAGCTG
  ATGAAGGGCCTGGGTGCTGGCGGGCGGCTCTGGGAGCTGCTGGAGAGACAACCACGGCTGCCTTTTAACG
  AGGGGATCGTGTTAGATGAGAAAACCTTCCAGGGCGCCCTGGAGTTCAGAAATGTGCATTTCACGTACCC
  TGCTCGCCCGGAGGTGTCCGTGTTCCAGGATTTCAGTCTTTCCATCCCATCTGGATCTGTCACAGCGCTG
  GTTGGCCCTAGTGGTTCTGGGAAATCAACGGTGGTCTCACTCCTGCTGCGGTTGTACGACCCCAGTTCTG
  GAACAGTCAGTCTTGATGGCCATGACATTCGTCAGCTAAACCCCGTCTGGCTGAGATCCAAGATTGGGAC
  AGTAAGTCAGGAGCCAGTCCTGTTCTCCTGCTCTGTTGCTGAAAACATTGCCTATGGTGCTGACAACCTC
  TCCTCGGTCACAGCCCGGCAGGTGGAGAGGGCCGCAGAAGTGGCCAATGCTGCTGAGTTCATTCGTAGCT
  TCCCACAGGGCTTCGACACTGTGGTCGGGGAGAAGGGTGTCCTCCTGTCTGGTGGGCAGAAGCAGAGGAT
  CGCAATAGCCAGAGCTCTGCTGAAGAACCCCAAAATTCTTCTCCTCGATGAAGCAACCAGCGCACTGGAT
  GCCGAAAACGAGCACCTGGTCCAGGAGGCGCTTGACCGGCTGATGGAGGGAAGAACCGTGTTGATCATAG
  CCCACCGCCTCTCCACCATTAAGAACGCCAATTTCGTTGCCGTCCTTAACCAAGGAAAGATCTGTGAACA
  TGGGACACATGAGGAGCTGCTTTTGAAGCCCAATGGGCTTTACAGAAAACTGATGAACAAGCAGAGTCTC
  CTGCTACATAACGGGGCAGAGCAGTTCCTGGAACCTGCACAGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206893 representing NM_001012166
  Red=Cloning site Green=Tags(s)
  
  MRAPPARALLLFPRRGPAVRAWAPAVSSRTWLASEWTPLVRAWTSLIHEPSPGLRFPVPLSGLPGGVGRW
  ATSSGIRRCWVLAGPRAAHPLFARLQGAAATGVRDLGNDSQRRPATTGRSEVWKLLGLVRPERGRLSAAV
  GFLAVSSVITMSAPFFLGKIIDVIYTNPTEDFGDSLTRLCAGLTCVFLCGAAANSIRVYLMQSSGQSIVN
  RLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTALLGHSVTENLSDGLRAVAQASVGVGMMFFVSPSLA
  TFVLSVVPPISVLAVIYGRYLRKLSKATQDSLAEATQLAEERIGNIRTIRAFGKEMTEVEKYTSRVDQLL
  QLARKEALARAGFFGAAGLSGNLIVLSVLYKGGLLLGSTHMTVGELSSFLMYAFWVGLSIGGLSSFYSQL
  MKGLGAGGRLWELLERQPRLPFNEGIVLDEKTFQGALEFRNVHFTYPARPEVSVFQDFSLSIPSGSVTAL
  VGPSGSGKSTVVSLLLRLYDPSSGTVSLDGHDIRQLNPVWLRSKIGTVSQEPVLFSCSVAENIAYGADNL
  SSVTARQVERAAEVANAAEFIRSFPQGFDTVVGEKGVLLSGGQKQRIAIARALLKNPKILLLDEATSALD
  AENEHLVQEALDRLMEGRTVLIIAHRLSTIKNANFVAVLNQGKICEHGTHEELLLKPNGLYRKLMNKQSL
  LLHNGAEQFLEPAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012166
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012166.1, NP_001012166.1
• RefSeq Size: 3460 bp
• RefSeq ORF: 2148 bp
• Locus ID: 361439
• Cytogenetics: 19q12
• MW: 77.4 kDa