Pla2g7 (NM_001009353) Rat Tagged ORF Clone

CAT#: RR206950

Pla2g7 (Myc-DDK-tagged ORF) - Rat phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) (Pla2g7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pla2g7
• Synonyms: MGC95107
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206950 representing NM_001009353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCGCTCAAACTGCGCGTGCTTTTCTGCCTCCTCTGCTGCCTCCCACGGGTACATCCTTTGTACT
  GGCAAGACCCTTCTTTTTTCGACTTCAGGCCATCAGTAATGTTTCATAAGCTCCAATCGGTGATGTCTGC
  TGTCAGCGCTGGCCGTTGTAAAATCCCCAAAGGAAATGGATCTTACCCTGTCGGCTGTACAGACATGATG
  TTCGGTTATGGGAATGAGAGCATCTTCTTGCGTTTGTACTACCCAGCTCAAGATCAAGGTCCTCATGACA
  CTGTTTGGGTCCCAAACATAGAATATTTTTGGGGTCTTAGTAAATTTCTCGGAACACCTACTTTTGTAGG
  CAACATTTTGCGCCTCTTATATGGTTCTCTGACAGCTCCTGCAAGCTGGAATTTTCCTTTGCGGACTGGA
  GAAAAATACCCACTAATTATCTTTTCTCACGGTCTCGGAGCCTTCAGGACAATTTATTCTGCTATTGGCG
  CTGCTTTGGCATCCTATGGGTTTATAGTGGCCACTGTGGAACATAGAGACGGATCTGCATCCGCAACTTA
  CTATTTTGAAGACCAGGCAGCTGCGAAAATGGAAAACAGGTCTTGGTTTTACCTGAAAAAGATAAAACAA
  GAGGAGTCGGAAAGAGCTCGGAAAGAGCAGGTTCGGCAGAGAGCAAAAGAATGTTCCAAGGCTCTCAGTG
  CAATTCTTGACATTGAACATGGAAACCCAAAAGAGAATGTACTAGGTTTACCTTTTGATATGAAACAGCT
  GAAGGATTCTATCGATGAGACTAAAATAGCTGTGATGGGGCATTCTTTTGGAGGAGCAACAGTTTTTCAA
  GCCCTAAGTGAAGACCAGAGATTCAGATGTGGGATTGCCCTTGATCCGTGGATGTTTCCCGTGAGTGAGG
  AGCTGTACTCCAGAGTTCCTCAGCCTCTCTTCTTTATCAACTCTGCCGAATTCCAGACTCCAAAGGACAT
  TGCAAAAATGAAAAACTTCTACCAGCCTGACAAGGAAAGGAAAATGATTACGATCAAGGGCTCAGTGCAC
  CAGAATTTTGCTGACGGGACTTTTGTAACTGGCAAAATAATTGGAAACAAGCTGTCACTGAAAGGAGACA
  TAGACTCCAGAGTTGCCATAGACCTCACCAACAAGGCTTCCTTGGCTTTCTTACAAAAACATTTAGGACT
  TCATAAAGACTTTGATCAGTGGGACTGTCTGGTGGAGGGAGAGAACGAGAACCTCATCCCGGGGTCACCC
  TTTGATGTAGTCACCCAGTCCCCGGCTCTGCAGAGTTCTCCCGGATCACACAACCAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206950 representing NM_001009353
  Red=Cloning site Green=Tags(s)
  
  MVPLKLRVLFCLLCCLPRVHPLYWQDPSFFDFRPSVMFHKLQSVMSAVSAGRCKIPKGNGSYPVGCTDMM
  FGYGNESIFLRLYYPAQDQGPHDTVWVPNIEYFWGLSKFLGTPTFVGNILRLLYGSLTAPASWNFPLRTG
  EKYPLIIFSHGLGAFRTIYSAIGAALASYGFIVATVEHRDGSASATYYFEDQAAAKMENRSWFYLKKIKQ
  EESERARKEQVRQRAKECSKALSAILDIEHGNPKENVLGLPFDMKQLKDSIDETKIAVMGHSFGGATVFQ
  ALSEDQRFRCGIALDPWMFPVSEELYSRVPQPLFFINSAEFQTPKDIAKMKNFYQPDKERKMITIKGSVH
  QNFADGTFVTGKIIGNKLSLKGDIDSRVAIDLTNKASLAFLQKHLGLHKDFDQWDCLVEGENENLIPGSP
  FDVVTQSPALQSSPGSHNQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009353
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009353.1, NP_001009353.1
• RefSeq Size: 1797 bp
• RefSeq ORF: 1323 bp
• Locus ID: 301265
• Cytogenetics: 9q13
• MW: 49.5 kDa