Mmp10 (NM_133514) Rat Tagged ORF Clone

CAT#: RR207171

Mmp10 (Myc-DDK-tagged ORF) - Rat matrix metallopeptidase 10 (Mmp10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207171 representing NM_133514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCACTGGCCATCCTGGTGCTGCTGTGCTTTCCGATCTGCTCAGCATATCCTCTGCATGGGGCAG
  TGAGACAAGACCACTCAACCATGGATCTTGCTCAGCAATACCTAGAAAAATACTACAACTTTAGAAAAAA
  TGAGAAACAATTTTTCAAAAGAAAGGACAGTAGTCCTGTTGTCAAAAAAATTGAAGAAATGCAGAAGTTC
  CTTGGGCTGGAGATGACAGGGAAGCTGGACTCGAACACTGTGGAGATGATGCACAAGCCCCGGTGTGGTG
  TTCCCGACGTTGGTGGCTTCAGTACCTTTCCAGGTTCACCCAAATGGAGGAAAAACCACATCTCCTACAG
  GATTGTGAATTATACACTGGATTTACCAAGAGAGAGTGTGGATTCTGCCATTGAGAGAGCTTTGAAGGTC
  TGGGAGGAGGTGACCCCACTCACATTCTCCAGGATCTCTGAAGGAGAGGCTGACATAATGATCTCCTTTG
  CAGTTGGAGAACATGGAGACTTTTACCCTTTTGATGGAGTGGGACAGAGCTTGGCTCATGCCTACCCACC
  TGGCCCTGGATTTTATGGAGATGCTCACTTCGATGATGATGAGAAATGGTCACTGGGACCCTCAGGGACC
  AATTTATTCCTGGTTGCTGCGCATGAACTTGGTCACTCCCTGGGTCTCTTTCACTCAAACAACAAAGAAT
  CTCTGATGTACCCAGTCTACAGGTTCTCCACGAGCCAAGCCAACATTCGCCTTTCTCAGGATGATATAGA
  GGGCATTCAATCCCTGTATGGAGCCCGCCCCTCCTCTGATGCCACAGTGGTTCCTGTGCCCTCTGTCTCT
  CCAAAACCTGAGACCCCAGTCAAATGTGATCCTGCTTTGTCCTTTGATGCAGTCACCATGCTGAGAGGGG
  AATTCCTATTCTTTAAAGACAGGCACTTCTGGCGTAGAACCCAGTGGAATCCCGAGCCTGAATTCCATTT
  GATTTCAGCATTTTGGCCCTCTCTTCCTTCAGGCTTAGATGCTGCCTATGAGGCAAATAACAAGGACAGA
  GTTCTGATTTTTAAAGGAAGTCAGTTCTGGGCAGTCCGAGGAAATGAAGTCCAAGCAGGTTACCCAAAGA
  GGATCCACACTCTTGGCTTTCCTCCCACCGTGAAGAAGATTGATGCAGCTGTTTTTGAAAAGGAGAAGAA
  GAAGACGTATTTCTTTGTAGGTGACAAATACTGGAGATTTGATGAGACAAGACAGCTTATGGATAAAGGC
  TTCCCGAGACTGATAACAGATGACTTCCCAGGAATTGAGCCACAAGTTGATGCTGTGTTACATGCATTTG
  GGTTTTTTTATTTCTTCTGTGGATCATCACAGTTCGAGTTTGACCCCAATGCCAGGACGGTGACACACAC
  ACTGAAGAGCAACAGCTGGCTGTTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207171 representing NM_133514
  Red=Cloning site Green=Tags(s)
  
  MEPLAILVLLCFPICSAYPLHGAVRQDHSTMDLAQQYLEKYYNFRKNEKQFFKRKDSSPVVKKIEEMQKF
  LGLEMTGKLDSNTVEMMHKPRCGVPDVGGFSTFPGSPKWRKNHISYRIVNYTLDLPRESVDSAIERALKV
  WEEVTPLTFSRISEGEADIMISFAVGEHGDFYPFDGVGQSLAHAYPPGPGFYGDAHFDDDEKWSLGPSGT
  NLFLVAAHELGHSLGLFHSNNKESLMYPVYRFSTSQANIRLSQDDIEGIQSLYGARPSSDATVVPVPSVS
  PKPETPVKCDPALSFDAVTMLRGEFLFFKDRHFWRRTQWNPEPEFHLISAFWPSLPSGLDAAYEANNKDR
  VLIFKGSQFWAVRGNEVQAGYPKRIHTLGFPPTVKKIDAAVFEKEKKKTYFFVGDKYWRFDETRQLMDKG
  FPRLITDDFPGIEPQVDAVLHAFGFFYFFCGSSQFEFDPNARTVTHTLKSNSWLLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133514
• ORF Size: 1428 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133514.1, NP_598198.1
• RefSeq Size: 1717
• RefSeq ORF: 1431
• Locus ID: 117061
• MW: 54.2 kDa
• Gene Summary: enzyme and a transformation-associated protein; activates procollagenase and degrades fibronectin and some gelatins [RGD, Feb 2006]