Sphk2 (NM_001012066) Rat Tagged ORF Clone

CAT#: RR207193

Sphk2 (Myc-DDK-tagged ORF) - Rat sphingosine kinase 2 (Sphk2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207193 representing NM_001012066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCGATCCTGCACGGCGAGTTTGGTTCCTACC
  CAGCCCATGGCCCCAGGTTTGCCCTCACCCTCACGACACAAGCCCTACACATACAGCGACTGCGCCCAAA
  GCCAGAAGCCCGGCCCCGAGATGGTCTAGTCCCTCTGGACGAGGTCTCAGGCTGTGGCACTCTGCAGAGC
  CGTAGCCCCGAAGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGGCGTGGGGGCCGGC
  GCAGAGCTACTCGTACCTTCCGGGCCGACGGGGCCGCCACTTATGTGGAGAATCGTGCAGAGGCCCAGCG
  TTGGGCAACTGCTCTCATGTGTCTCCTCCGAGGAGTGCCTCTGTCTGGGGACCAGGAAATCACCCCTGAA
  TTGCTGCCCCGGAAGCCCAGGCTGCTCCTATTGGTCAATCCTTTTGGGGGGCGGGGCCTGGCCTGGCAGC
  GCTGTATGGACCACGTGGTGCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACG
  ACAAAACCATGCACGTGAGCTGGTGCAGGGGTTGAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCC
  GGAGATGGGCTGCTTTATGAGGTGCTGAATGGGCTCCTCGACCGCCCAGATTGGGAAGACGCTGTGCGGA
  TGCCCATTGGTGTCCTCCCCTGTGGTTCGGGCAATGCACTAGCTGGGGCAGTTAACCATCATGGCGGGTT
  TGAGCAAACTGTTGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGGGGTGGCAGCCATCCT
  CTGGACCTGCTCTCTGTGACGCTAGCCTCAGGATCCCGCTGTTTTTCCTTCCTGTCAGTTGCCTGGGGAT
  TCTTGTCAGATGTGGACATTCACAGCGAGCGCTTCAGAGCCCTGGGCAGTGCTCGATTCACACTGGGTGC
  AGTGCTAGGCCTGGCCACATTGCATACCTACCGTGGACGTCTCTCCTACCTCCCTGCTACCACAGAACCA
  GCCTTGCCCATCCCAGGCCATGGTCTGCCAAGAGCCAAGTCAGAACTAGCCTTGGCCCCAGCCCCAGCCC
  CAGCCGCCACCCACTCACCTCTTCATCGATCTGTGTCTGACCTGCCCCTGCCTCTTCCCCAGCCTGCCCT
  GGCCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGA
  GACTGGGGGGGAGCTGGGGATGCACCTTTGTCCCCAGACCCATTGCTGCCTTCATCCCCCAACGCTTTCA
  AAACAGCTCAGCTTTCACCCATCGCCGAAGGGCCCTCAGAAATGCCAGCATCAGGGCTGCTGCCTCCCAT
  CCACAATGCTCCAGAAGCCTCTACCTGGGGCCCAGCGGACCACCTGCTCCCTCCCCTGGGCTCTCCACTG
  CCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGCCATCTTGCCAAGCCACCTCTGCG
  CAGACCTGATGGCAGCCCCACACGCGCGTTTTGACGATGGTGTGGTGCACCTGTGCTGGGTGCGGAGCGG
  CATCTCACGGGCCGCACTTCTACGAATTTTGCTGGCCATGGAACATGGAAACCACTTCAGCCTGGGCTGC
  CCACATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAGCCACTCACGCCTCGTGGGGTGCTCACTG
  TAGATGGGGAATTAGTAGAGTATGGGCCAATACAGGCACAGGTGCATCCAAGTCTCGCCACACTGCTCAC
  TGGGCCTGCAGGTCAAAAGCCTCGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207193 representing NM_001012066
  Red=Cloning site Green=Tags(s)
  
  MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQS
  RSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPE
  LLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVS
  GDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHP
  LDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEP
  ALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTG
  DWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPL
  PQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGC
  PHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012066
• ORF Size: 1848 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012066.1, NP_001012066.1
• RefSeq Size: 2408 bp
• RefSeq ORF: 1851 bp
• Locus ID: 308589
• Cytogenetics: 1q22
• MW: 65.5 kDa