Sphk2 (NM_001012066) Rat Untagged Clone

CAT#: RN207193

Sphk2 (untagged ORF) - Rat sphingosine kinase 2 (Sphk2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Sphk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207193 representing NM_001012066
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCGATCCTGCACGGCGAGTTTGGTTCCTACC
  CAGCCCATGGCCCCAGGTTTGCCCTCACCCTCACGACACAAGCCCTACACATACAGCGACTGCGCCCAAA
  GCCAGAAGCCCGGCCCCGAGATGGTCTAGTCCCTCTGGACGAGGTCTCAGGCTGTGGCACTCTGCAGAGC
  CGTAGCCCCGAAGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGGCGTGGGGGCCGGC
  GCAGAGCTACTCGTACCTTCCGGGCCGACGGGGCCGCCACTTATGTGGAGAATCGTGCAGAGGCCCAGCG
  TTGGGCAACTGCTCTCATGTGTCTCCTCCGAGGAGTGCCTCTGTCTGGGGACCAGGAAATCACCCCTGAA
  TTGCTGCCCCGGAAGCCCAGGCTGCTCCTATTGGTCAATCCTTTTGGGGGGCGGGGCCTGGCCTGGCAGC
  GCTGTATGGACCACGTGGTGCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACG
  ACAAAACCATGCACGTGAGCTGGTGCAGGGGTTGAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCC
  GGAGATGGGCTGCTTTATGAGGTGCTGAATGGGCTCCTCGACCGCCCAGATTGGGAAGACGCTGTGCGGA
  TGCCCATTGGTGTCCTCCCCTGTGGTTCGGGCAATGCACTAGCTGGGGCAGTTAACCATCATGGCGGGTT
  TGAGCAAACTGTTGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGGGGTGGCAGCCATCCT
  CTGGACCTGCTCTCTGTGACGCTAGCCTCAGGATCCCGCTGTTTTTCCTTCCTGTCAGTTGCCTGGGGAT
  TCTTGTCAGATGTGGACATTCACAGCGAGCGCTTCAGAGCCCTGGGCAGTGCTCGATTCACACTGGGTGC
  AGTGCTAGGCCTGGCCACATTGCATACCTACCGTGGACGTCTCTCCTACCTCCCTGCTACCACAGAACCA
  GCCTTGCCCATCCCAGGCCATGGTCTGCCAAGAGCCAAGTCAGAACTAGCCTTGGCCCCAGCCCCAGCCC
  CAGCCGCCACCCACTCACCTCTTCATCGATCTGTGTCTGACCTGCCCCTGCCTCTTCCCCAGCCTGCCCT
  GGCCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGA
  GACTGGGGGGGAGCTGGGGATGCACCTTTGTCCCCAGACCCATTGCTGCCTTCATCCCCCAACGCTTTCA
  AAACAGCTCAGCTTTCACCCATCGCCGAAGGGCCCTCAGAAATGCCAGCATCAGGGCTGCTGCCTCCCAT
  CCACAATGCTCCAGAAGCCTCTACCTGGGGCCCAGCGGACCACCTGCTCCCTCCCCTGGGCTCTCCACTG
  CCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGCCATCTTGCCAAGCCACCTCTGCG
  CAGACCTGATGGCAGCCCCACACGCGCGTTTTGACGATGGTGTGGTGCACCTGTGCTGGGTGCGGAGCGG
  CATCTCACGGGCCGCACTTCTACGAATTTTGCTGGCCATGGAACATGGAAACCACTTCAGCCTGGGCTGC
  CCACATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAGCCACTCACGCCTCGTGGGGTGCTCACTG
  TAGATGGGGAATTAGTAGAGTATGGGCCAATACAGGCACAGGTGCATCCAAGTCTCGCCACACTGCTCAC
  TGGGCCTGCAGGTCAAAAGCCTCGAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012066
• Insert Size: 1851 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012066.1, NP_001012066.1
• RefSeq Size: 2408 bp
• RefSeq ORF: 1851 bp
• Locus ID: 308589
• Cytogenetics: 1q22