Pfkfb1 (NM_012621) Rat Tagged ORF Clone

CAT#: RR207195

Pfkfb1 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb1
• Synonyms: Pfkfb01; PFRX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207195 representing NM_012621
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGAGAGATGGGAGAACTCACTCAAACCAGGTTACAGAAGATCTGGATTCCACACAGCAGCAGTA
  GCAGCGTGCTGCAACGGCGAAGGGGCTCCTCCATACCACAGTTCACTAATTCTCCCACGATGGTGATCAT
  GGTGGGTTTACCAGCTCGAGGCAAGACCTACATCTCTACGAAGCTCACACGCTATCTCAACTGGATAGGA
  ACACCAACTAAAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGTTACAGGAACTATGAATTCT
  TTCGCCCAGACAACACAGAGGCCCAGCTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAGGATGTCCA
  TAAGTATCTCAGCCGCGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACGA
  TCGTTGATTCTACAGTTTGCTAAGGAACATGGTTATAAGGTCTTCTTTATTGAGTCTATTTGTAATGACC
  CCGAAATCATTGCAGAAAACATCAAGCAAGTGAAACTTGGTAGTCCTGATTACATAGACTGTGACCAAGA
  AAAGGTTTTGGAAGACTTTCTAAAGAGAATAGAGTGCTATGAGATCAACTACCAACCTTTGGATGAGGAA
  TTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTAAATCGAGTGCAGG
  ACCACGTTCAGAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATCTACCTATG
  CCGCCATGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAGCTCGGGGC
  AAGCAGTATGCCTATGCACTAGCCAACTTCATCCGGTCTCAAGGCATCAGCTCCCTGAAAGTATGGACTA
  GCCACATGAAGAGGACCATTCAGACCGCTGAAGCCCTAGGTGTCCCCTATGAACAGTGGAAGGCCCTGAA
  TGAGATTGATGCGGGTGTCTGTGAAGAGATGACCTATGAAGAAATTCAGGAACACTACCCTGAGGAATTT
  GCACTACGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGC
  GTCTTGAACCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAGGCTGTCAT
  GCGGTGCCTCCTGGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTCCTCTGCAT
  ACTGTGCTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGTGGAGGCTG
  TGAACACACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAAGCTGAGGAAGCCTTGGACACTGT
  ACCTGCCCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207195 representing NM_012621
  Red=Cloning site Green=Tags(s)
  
  MSREMGELTQTRLQKIWIPHSSSSSVLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIG
  TPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERR
  SLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEE
  LDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARG
  KQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEF
  ALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLH
  TVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012621
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012621.1, NM_012621.3, NM_012621.4, NP_036753.4
• RefSeq Size: 1830
• RefSeq ORF: 1416
• Locus ID: 24638
• MW: 54.8 kDa
• Gene Summary: bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]