Idh3g (NM_031551) Rat Tagged ORF Clone

CAT#: RR207374

Idh3g (Myc-DDK-tagged ORF) - Rat isocitrate dehydrogenase 3 (NAD), gamma (Idh3g), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Idh3g
• Synonyms: IDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207374 representing NM_031551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGAAGGTGGCGATAGCTGCTGGCAGTGCTGCAAAGGCAATATTCAAGCCAGCTCTCCTCTGCC
  GTCCTTGGGAGGTTCTGGCTGCCCATGAGGCCCCCCGAAGGAGCATTTCCTCACAACAAACAATTCCTCC
  GTCTGCTAAGTATGGTGGGCGGCATACAGTGACTATGATTCCAGGGGATGGCATCGGCCCGGAGCTCATG
  TTGCATGTTAAGTCGGTATTCAGGCATGCATGTGTGCCAGTGGACTTTGAAGAGGTGCATGTAAGCTCCA
  ACGCTGATGAGGAGGACATCCGCAATGCCATCATGGCCATCCGCCGGAACCGTGTGGCTCTAAAGGGCAA
  CATCGAAACAAATCATAACTTGCCACCATCCCACAAATCCCGGAACAACATCCTTCGTACCAGCCTAGAC
  CTCTATGCCAACGTCATCCACTGTAAGAGTCTGCCAGGAGTGGTGACCCGGCACAAGGACATAGACATCC
  TCATTGTGCGGGAAAACACAGAAGGCGAGTACAGCAGCCTGGAGCATGAGAGTGTAGCAGGAGTGGTGGA
  GAGCTTGAAGATTATCACCAAAGCCAAGTCCCTGCGCATTGCTGAATATGCTTTCAAGCTGGCCCAGGAG
  AGTGGGCGTAAGAAAGTGACGGCTGTGCACAAGGCCAACATCATGAAACTGGGTGATGGACTCTTCCTCC
  AGTGTTGCAGGGAAGTGGCAGCCCGCTACCCTCAGATCACCTTTGATAGCATGATTGTGGACAACACAAC
  AATGCAGCTGGTATCCCGGCCTCAGCAGTTTGATGTCATGGTGATGCCTAATCTCTATGGTAACATTGTC
  AACAACGTCTGTGCAGGGCTAGTTGGAGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTATG
  CAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTGCCAATAAGAACATTGCTAACCCTACTGC
  CACATTGCTAGCAAGTTGCATGATGCTAGACCACCTCAAGCTCCATTCCTATGCCACCTCCATCCGAAAA
  GCTGTCTTAGCATCCATGGACAATGAAAATATGCATACCCCAGACATTGGAGGCCAGGGCACCACATCCC
  AAGCCATCCAGGACATCATTCGTCACATCCGCATCATTAATGGACGGGCTGTGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207374 representing NM_031551
  Red=Cloning site Green=Tags(s)
  
  MALKVAIAAGSAAKAIFKPALLCRPWEVLAAHEAPRRSISSQQTIPPSAKYGGRHTVTMIPGDGIGPELM
  LHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLD
  LYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE
  SGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV
  NNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRK
  AVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRIINGRAVEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031551
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031551.1, NP_113739.1
• RefSeq Size: 1371
• RefSeq ORF: 1182
• Locus ID: 25179
• MW: 42.9 kDa
• Gene Summary: gamma subunit of NAD(+)-isocitrate dehydrogenase, which catalyzes oxidative decarboxylation of citrate, and may function in the TCA cycle [RGD, Feb 2006]