Txnrd2 (NM_022584) Rat Tagged ORF Clone

CAT#: RR207441

Txnrd2 (Myc-DDK-tagged ORF) - Rat thioredoxin reductase 2 (Txnrd2), nuclear gene encoding mitochondrial protein, (10 ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Symbol: Txnrd2
• Synonyms: Tr3; Trxr2; Trxrd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207441 representing NM_022584
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATTGTGGCGGCGCTGCGCGGATCCAGCGGGCGCTTCCGGCCGCAGACACGGGTTTTAACAC
  GCGGGACGCGGGGCGCGGCGGGCGCGGCGAGCGCAGCGGGAGGGCAGCAGAACTTTGATCTCTTGGTGAT
  CGGTGGGGGATCCGGTGGCCTAGCTTGTGCCAAGGAAGCGGCTCAGCTGGGAAGGAAGGTGGCTGTGGCT
  GACTATGTGGAACCCTCTCCCCGAGGCACCAAATGGGGCCTTGGTGGCACCTGTGTCAACGTGGGCTGCA
  TACCCAAGAAGCTGATGCATCAGGCCGCACTGCTGGGGGGCATGATCAGAGATGCTCAGCACTACGGCTG
  GGAGGTGGCCCAGCCTGTCCAGCACAACTGGAAGGCAATGGCCGAAGCCGTGCAAAACCATGTGAAGTCC
  TTGAACTGGGGTCATCGTGTCCAACTGCAGGACAGGAAAGTCAAGTACTTTAACATCAAAGCCAGCTTTG
  TCAACGAGCACACAGTTCACGGTGTCGACAAAGCCGGGAAGGTGACTCAGCTTTCAGCCAAGCACATAGT
  CATCGCTACAGGAGGACGGCCGAAGTACCCCACACAGGTCAAAGGAGCCCTGGAACACGGAATCACAAGT
  GATGACATCTTCTGGCTGAAGGAGTCCCCTGGGAAAACGTTGGTGGTTGGAGCCAGTTATGTGGCCCTGG
  AGTGTGCCGGCTTCCTCACTGGTATTGGCCTGGATACCACGGTCATGATGCGCAGCGTGCCCCTCCGAGG
  CTTTGACCAGCAAATGGCGTCTTTGGTCACAGAGCACATGGAGTCTCATGGCACCCGGTTCCTGAAAGGC
  TGTGTCCCCTCCCTCATCAGAAAACTCCCGACTAACCAACTGCAGGTCACTTGGGAGGATCTCGCTTCTG
  GCAAGGAGGACGTGGGCACCTTTGACACTGTCCTGTGGGCCATAGGGCGAGTTCCAGAGACCAGAAATTT
  GAATCTGGAGAAGGCTGGCGTTAATACCAACCCTAAGAATCAGAAGATCATTGTGGATGCCCAGGAGGCC
  ACCTCTGTCCCCCACATCTATGCCATTGGAGATGTTGCTGAGGGGCGGCCTGAGCTGACACCCACAGCTA
  TCAAGGCAGGAAAGCTTCTGGCTCAGCGGCTCTTTGGGAAATCCTCAACCTTAATGAATTACAGCAACGT
  CCCCACAACTGTCTTTACACCACTGGAGTATGGCTGTGTGGGACTGTCTGAGGAGGAGGCTGTGGCTCTC
  CACGGCCAGGAGCATATAGAGGTTTACCATGCATATTACAAGCCCCTAGAGTTCACAGTGGCAGATCGGG
  ATGCATCACAGTGCTACATAAAGATGGTATGCATGAGGGAGCCCCCACAACTGGTACTGGGCCTGCACTT
  CCTTGGCCCCAACGCTGGAGAAGTCACACAAGGATTTGCTCTTGGGATCCAGTGTGGGGCTTCATACGCA
  CAGGTGATGCAGACAGTAGGGATCCACCCCACCTGCTCTGAGGAGGTGGTTAAGCTGCACATCTCCAAGC
  GCTCTGGCCTGGATCCTACTGTGACTGGCTGCTGAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207441 representing NM_022584
  Red=Cloning site Green=Tags(s)
  
  MAAIVAALRGSSGRFRPQTRVLTRGTRGAAGAASAAGGQQNFDLLVIGGGSGGLACAKEAAQLGRKVAVA
  DYVEPSPRGTKWGLGGTCVNVGCIPKKLMHQAALLGGMIRDAQHYGWEVAQPVQHNWKAMAEAVQNHVKS
  LNWGHRVQLQDRKVKYFNIKASFVNEHTVHGVDKAGKVTQLSAKHIVIATGGRPKYPTQVKGALEHGITS
  DDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTVMMRSVPLRGFDQQMASLVTEHMESHGTRFLKG
  CVPSLIRKLPTNQLQVTWEDLASGKEDVGTFDTVLWAIGRVPETRNLNLEKAGVNTNPKNQKIIVDAQEA
  TSVPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTLMNYSNVPTTVFTPLEYGCVGLSEEEAVAL
  HGQEHIEVYHAYYKPLEFTVADRDASQCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIQCGASYA
  QVMQTVGIHPTCSEEVVKLHISKRSGLDPTVTGC*G
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022584
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_022584.1, NM_022584.2, NM_022584.3, NP_072106.1
• RefSeq Size: 2011
• RefSeq ORF: 1581
• Locus ID: 50551
• MW: 56.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2017]