Ctcf (NM_031824) Rat Tagged ORF Clone

CAT#: RR207558

Ctcf (Myc-DDK-tagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctcf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207558 representing NM_031824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTGAGGCGGTTGAAGCCATTGTGGAAGAGTCTGAAACTTTCATTAAAGGAAAGGAAAGAAAGA
  CTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCTTGCCACCTACCCCAGAACCAGACAGACGG
  GGGTGAGGTGGTCCAGGATGTCAACAGTAGCGTACAGATGGTAATGATGGAACAGCTGGACCCTACCCTT
  CTCCAAATGAAGACTGAAGTAATGGAGGGGACAGTGGCTCCTGAAGCAGAAGCTGCAGTGGACGATACCC
  AAATCATAACCCTGCAGGTTGTAAACATGGAGGAACAGCCCATTAATATAGGAGAACTTCAGCTTGTCCA
  AGTACCTGTTCCTGTGACTGTACCTGTTGCTACTACGTCAGTAGAAGAACTTCAGGGGGCTTATGAGAAT
  GAAGTGTCTAAAGAGGGCCTTGCAGAAAGTGAACCGATGATATGTCACACCTTACCTTTACCTGAAGGAT
  TTCAGGTGGTAAAAGTGGGGGCCAATGGAGAAGTGGAGACACTAGAGCAGGGGGAGCTTCCTCCTCAGGA
  AGACCCTAGCTGGCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCAAAAAGAGT
  AAACTTCGTTACACAGAGGAGGGCAAAGACGTGGATGTGTCTGTGTATGATTTTGAGGAAGAACAGCAGG
  AAGGACTGCTGTCTGAGGTTAATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCGAAGCCAACAAAAAT
  TAAAAAAAAAGGTGTAAAGAAAACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT
  TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGCAGAGCATTCA
  GAACAGTAACCCTCCTGAGGAATCATCTTAACACACACACAGGTACTCGTCCTCACAAGTGCCCCGACTG
  CGACATGGCTTTTGTGACCAGTGGAGAGTTGGTGCGGCATCGTCGTTATAAACACACTCATGAGAAACCA
  TTTAAGTGTTCCATGTGTGATTATGCCAGTGTAGAAGTCAGCAAATTAAAACGACACATTCGCTCTCATA
  CTGGAGAGCGCCCGTTCCAGTGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCA
  TATGAGAACCCATTCAGGTGAAAAACCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAGAGTGGT
  ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCATTGTCCCCACTGTGACA
  CTGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGAGAAAGCAGCATTCCTATATTGAGCAGGGCAA
  AAAATGTCGCTACTGTGATGCTGTGTTTCATGAGAGATATGCTCTTATCCAGCATCAAAAATCACACAAA
  AATGAGAAGCGCTTCAAGTGCGACCAGTGTGATTATGCTTGTAGACAGGAGAGGCACATGATCATGCACA
  AGCGCACTCACACGGGGGAGAAGCCTTATGCCTGCAGCCACTGCGACAAGACCTTCCGCCAGAAACAGCT
  CCTTGACATGCATTTCAAGCGCTATCATGATCCCAACTTTGTCCCCGCTGCCTTTGTCTGTTCTAAGTGT
  GGGAAAACATTCACACGCCGGAACACAATGGCGAGACACGCAGATAACTGTGCTGGTCCAGACGGTGTAG
  AAGGGGAAAATGGAGGAGAGACAAAGAAGAGCAAACGAGGAAGAAAAAGAAAGATGCGGTCTAAAAAAGA
  AGACTCCTCTGACAGTGAAAATGCTGAGCCGGATCTCGATGACAATGAGGAGGAGGAGGAGCCTGCTGTA
  GAAATTGAACCTGAGCCAGAGCCGCAGCCGCAGCCTCAGCCACAGCCGCAGCCGCAGCCTGTGGCCCCAG
  CCCCACCACCTGCCAAGAAGAGAAGAGGGCGACCTCCTGGAAGAACCAACCAACCCAAACAGAACCAGCC
  AACAGCCATCATCCAGGTTGAAGACCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAGTCAAAAAA
  GAGCCAGATGCCGAGCCTGCGGAGGGGGAAGAGGAGGAGGCTCAGGCAGCACCCGCAGACGCCCCCAACG
  GAGACCTCACGCCTGAGATGATTCTCAGCATGATGGACCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207558 representing NM_031824
  Red=Cloning site Green=Tags(s)
  
  MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTL
  LQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYEN
  EVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKS
  KLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSN
  LDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKP
  FKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSG
  TMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHK
  NEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKC
  GKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEEEEEPAV
  EIEPEPEPQPQPQPQPQPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKK
  EPDAEPAEGEEEEAQAAPADAPNGDLTPEMILSMMDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031824
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031824.1, NP_114012.1
• RefSeq Size: 3708
• RefSeq ORF: 2214
• Locus ID: 83726
• MW: 83.9 kDa
• Gene Summary: binds to and protects the H19 differentially methylated domain from de novo methylation during oocyte growth and is involved in normal preimplantation development [RGD, Feb 2006]