Wasf1 (NM_001025114) Rat Tagged ORF Clone

CAT#: RR207646

Wasf1 (Myc-DDK-tagged ORF) - Rat WAS protein family, member 1 (Wasf1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wasf1
• Synonyms: MGC109303
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207646 representing NM_001025114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGGTGAAAAGAAACATCGACCCTAGGCACTTGTGCCACACAGCACTGCCCAGGGGCATTAAGA
  ACGAGCTGGAATGTGTGACCAACATCTCCCTGGCAAATATAATTAGACAACTGAGTAGCCTAAGTAAATA
  TGCTGAAGACATATTTGGAGAATTATTCAATGAAGCACATAGTTTTTCCTTCAGAGTTAACTCATTACAA
  GAGCGCGTGGACCGATTGTCCGTTAGTGTCACACAGCTTGATCCTAAAGAAGAAGAACTGTCCTTACAAG
  ACATAACGATGAGAAAAGCTTTCCGGAGCTCTACAATTCAGGACCAGCAGCTTTTCGACCGCAAGACTTT
  GCCTATCCCGCTGCAGGAAACGTACGATGTGTGCGAGCAGCCTCCGCCTCTCAATGTCCTCACACCCTAC
  AGGGACGATGGTAAGGAGGGGCTGAAGTTTTATACCAACCCTTCGTATTTCTTTGATCTGTGGAAAGAAA
  AGATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGAAAGCAGAAGCAGAAAAATCTAGACCGTCC
  TCATGAACCAGAAAAAGTGCCAAGAGCACCACATGACAGGCGGCGAGAATGGCAGAAGCTGGCCCAAGGT
  CCAGAGCTGGCTGAAGATGATGCCGATCTCCTCCACAAGCACATCGAAGTTGCTAACGGCCCAGCCTCTC
  ATTTTGAGACAAGGCCGCAGACATACGTGGACCACATGGATGGATCTTACTCACTCTCTGCCTTGCCATT
  CAGTCAGATGAGTGAACTCCTAAGCAGAGCTGAGGAGAGGGTCTTAGTCAGACCCCATGAACCACCTCCA
  CCTCCACCAATGCATGCAGCAGGGGATGCAAGACCCACCCCCACCTGTGTCAGCTCTGCTGCGGGTTTGA
  TAGAGAATCGTCCCCAGTCCCCAGCTGCAGGCAGAACACCCGTGTTTGTGAGCCCCACGCCCCCACCTCC
  TCCACCACCTCTCCCGTCTGCCCTGTCCACCTCCTCACTGAGAGCTTCCATGACCTCAACTCCTCCCCCA
  CCGGTGCCTCCCCCGCCTCCTCCTCCAGCCCCTGCCTTGCAGGCCCCAGCAGTGCCCCCGCCTCCAGCCC
  CTCTTCAGATTGCCCCTGGTGTCCTTCACCCAGCTCCTCCTCCAATCGCTCCTCCTCTAGTACAGCCCTC
  TCCCCCGGTAGCCAGAGCCGCCCCAGTGTGTGAGACTGTACCCGTTCATCCACTCCCACAAGGTGAAGTG
  CAGGGGCTGCCTCCGCCCCCGCCGCCGCCGCCTCTGCCTCCTCCAGGCATTCGGCCATCATCACCTGTCA
  CGGTCGCAGCTCTTGCGCATCCTCCCTCTGGGCTCCACCCAACTCCATCTCCTGCCCCTGGTCCCCATGC
  TCCATTAATGCCTCCGTCCCCACCTTCACAAGTCCTGCCTGCCTCTGAGCCAAAGCGCCATCCTTCCACC
  CTACCCGTGATCAGTGACGCGAGGAGTGTGCTGCTGGAGGCCATACGGAAAGGCATTCAGCTTCGCAAAG
  TGGAAGAGCAGCGTGAACAGGAAGCAAAGCATGAGCGGATTGAAAACGACGTGGCCACCATCCTGTCACG
  CCGCATCGCTGTGGAGTACAGCGACTCGGAAGACGACTCGGAGTTCGATGAAGTAGACTGGCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207646 representing NM_001025114
  Red=Cloning site Green=Tags(s)
  
  MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQ
  ERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPY
  RDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQG
  PELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPP
  PPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPP
  PVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEV
  QGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPST
  LPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025114
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025114.1, NP_001020285.1
• RefSeq Size: 2579 bp
• RefSeq ORF: 1680 bp
• Locus ID: 294568
• Cytogenetics: 20q13
• MW: 61.5 kDa