Cyp27a1 (NM_178847) Rat Tagged ORF Clone

CAT#: RR207658

Cyp27a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp27a1
• Synonyms: Cyp27; P450C27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207658 representing NM_178847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGTTGAGCCGCATGAGACTGAGATGGGCGCTTCTGGACACTCGTGTGATGGGCCATGGCCTCT
  GCCCACAAGGGGCCAGAGCCAAGGCCGCGATCCCTGCAGCCCTCCGGGATCACGAGAGCACGGAGGGTCC
  AGGAACAGGTCAAGACCGACCGCGCCTGCGGAGTCTGGCGGAGCTTCCGGGACCCGGAACGCTACGCTTT
  TTATTCCAGCTATTTCTACGAGGCTATGTGCTGCACTTGCACGAGCTCCAGGCGCTGAACAAGGCCAAGT
  ACGGCCCAATGTGGACAACCACCTTTGGGACTCGCACCAATGTGAATCTGGCTAGCGCCCCGCTCTTGGA
  GCAAGTGATGAGACAGGAGGGCAAGTACCCCATAAGAGACAGCATGGAGCAGTGGAAGGAGCACCGAGAC
  CACAAAGGCCTCTCCTATGGGATCTTCATCACACAAGGACAGCAGTGGTACCATCTGCGTCATAGTTTGA
  ATCAGCGGATGCTGAAGCCTGCTGAGGCAGCCCTCTACACAGATGCCTTAAACGAGGTCATCAGTGACTT
  TATTGCCCGGCTGGACCAGGTGCGGACAGAGAGTGCATCAGGGGATCAGGTGCCAGATGTGGCACATCTT
  CTCTACCACCTTGCCTTGGAAGCCATCTGCTATATCCTGTTTGAGAAAAGGGTTGGCTGCCTGGAGCCCT
  CCATCCCTGAGGACACCGCCACCTTCATCAGATCTGTTGGACTCATGTTCAAGAACTCAGTCTATGTCAC
  TTTCCTTCCCAAGTGGTCTCGGCCTCTGCTGCCCTTTTGGAAGCGATACATGAATAACTGGGATAACATT
  TTCTCCTTCGGGGAGAAGATGATTCATCAAAAAGTCCAGGAGATAGAAGCCCAGCTACAGGCGGCTGGGC
  CAGATGGGGTCCAGGTATCTGGCTACCTGCACTTCCTGCTGACTAAGGAATTGCTCAGTCCTCAAGAGAC
  TGTCGGCACCTTTCCTGAGCTGATCTTGGCTGGGGTAGACACGACATCCAATACACTGACCTGGGCCCTG
  TATCACCTTTCAAAGAACCCAGAGATCCAGGAAGCCTTGCACAAGGAAGTGACTGGTGTGGTACCCTTCG
  GGAAGGTGCCCCAGAACAAGGACTTTGCCCACATGCCCCTGCTAAAAGCTGTGATTAAGGAGACCCTGCG
  CCTCTACCCTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTCCTC
  TTCCCTAAGAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGAGATCCCAGTGTCTTTCCTGAGC
  CCGAGAGCTTCCAGCCTCACCGATGGCTGAGGAAGAGAGAGGACGATAACTCCGGGATCCAACACCCATT
  TGGCTCTGTGCCCTTTGGCTATGGGGTTCGGTCCTGCCTGGGTCGCAGGATTGCAGAACTGGAGATGCAA
  CTCCTGCTGTCAAGGCTGATACAAAAGTATGAGGTGGTCCTGTCTCCCGGGATGGGAGAAGTGAAGTCTG
  TGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTGAGCCTACGCTTTCTGCAGAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207658 representing NM_178847
  Red=Cloning site Green=Tags(s)
  
  MAVLSRMRLRWALLDTRVMGHGLCPQGARAKAAIPAALRDHESTEGPGTGQDRPRLRSLAELPGPGTLRF
  LFQLFLRGYVLHLHELQALNKAKYGPMWTTTFGTRTNVNLASAPLLEQVMRQEGKYPIRDSMEQWKEHRD
  HKGLSYGIFITQGQQWYHLRHSLNQRMLKPAEAALYTDALNEVISDFIARLDQVRTESASGDQVPDVAHL
  LYHLALEAICYILFEKRVGCLEPSIPEDTATFIRSVGLMFKNSVYVTFLPKWSRPLLPFWKRYMNNWDNI
  FSFGEKMIHQKVQEIEAQLQAAGPDGVQVSGYLHFLLTKELLSPQETVGTFPELILAGVDTTSNTLTWAL
  YHLSKNPEIQEALHKEVTGVVPFGKVPQNKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFL
  FPKNTQFVLCHYVVSRDPSVFPEPESFQPHRWLRKREDDNSGIQHPFGSVPFGYGVRSCLGRRIAELEMQ
  LLLSRLIQKYEVVLSPGMGEVKSVSRIVLVPSKKVSLRFLQRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178847
• ORF Size: 1599 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178847.1, NM_178847.2, NM_178847.3, NP_849178.2
• RefSeq Size: 2150
• RefSeq ORF: 1602
• Locus ID: 301517
• MW: 60.7 kDa
• Gene Summary: mitochondrial enzyme with both vitamin D3 25-hydroxylase and cholesterol 26-hydroxylase activities [RGD, Feb 2006]