Cyp27a1 (NM_178847) Rat Untagged Clone

CAT#: RN207658

Cyp27a1 (untagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cyp27a1
• Synonyms: Cyp27; P450C27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207658 representing NM_178847
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGTTGAGCCGCATGAGACTGAGATGGGCGCTTCTGGACACTCGTGTGATGGGCCATGGCCTCT
  GCCCACAAGGGGCCAGAGCCAAGGCCGCGATCCCTGCAGCCCTCCGGGATCACGAGAGCACGGAGGGTCC
  AGGAACAGGTCAAGACCGACCGCGCCTGCGGAGTCTGGCGGAGCTTCCGGGACCCGGAACGCTACGCTTT
  TTATTCCAGCTATTTCTACGAGGCTATGTGCTGCACTTGCACGAGCTCCAGGCGCTGAACAAGGCCAAGT
  ACGGCCCAATGTGGACAACCACCTTTGGGACTCGCACCAATGTGAATCTGGCTAGCGCCCCGCTCTTGGA
  GCAAGTGATGAGACAGGAGGGCAAGTACCCCATAAGAGACAGCATGGAGCAGTGGAAGGAGCACCGAGAC
  CACAAAGGCCTCTCCTATGGGATCTTCATCACACAAGGACAGCAGTGGTACCATCTGCGTCATAGTTTGA
  ATCAGCGGATGCTGAAGCCTGCTGAGGCAGCCCTCTACACAGATGCCTTAAACGAGGTCATCAGTGACTT
  TATTGCCCGGCTGGACCAGGTGCGGACAGAGAGTGCATCAGGGGATCAGGTGCCAGATGTGGCACATCTT
  CTCTACCACCTTGCCTTGGAAGCCATCTGCTATATCCTGTTTGAGAAAAGGGTTGGCTGCCTGGAGCCCT
  CCATCCCTGAGGACACCGCCACCTTCATCAGATCTGTTGGACTCATGTTCAAGAACTCAGTCTATGTCAC
  TTTCCTTCCCAAGTGGTCTCGGCCTCTGCTGCCCTTTTGGAAGCGATACATGAATAACTGGGATAACATT
  TTCTCCTTCGGGGAGAAGATGATTCATCAAAAAGTCCAGGAGATAGAAGCCCAGCTACAGGCGGCTGGGC
  CAGATGGGGTCCAGGTATCTGGCTACCTGCACTTCCTGCTGACTAAGGAATTGCTCAGTCCTCAAGAGAC
  TGTCGGCACCTTTCCTGAGCTGATCTTGGCTGGGGTAGACACGACATCCAATACACTGACCTGGGCCCTG
  TATCACCTTTCAAAGAACCCAGAGATCCAGGAAGCCTTGCACAAGGAAGTGACTGGTGTGGTACCCTTCG
  GGAAGGTGCCCCAGAACAAGGACTTTGCCCACATGCCCCTGCTAAAAGCTGTGATTAAGGAGACCCTGCG
  CCTCTACCCTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTCCTC
  TTCCCTAAGAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGAGATCCCAGTGTCTTTCCTGAGC
  CCGAGAGCTTCCAGCCTCACCGATGGCTGAGGAAGAGAGAGGACGATAACTCCGGGATCCAACACCCATT
  TGGCTCTGTGCCCTTTGGCTATGGGGTTCGGTCCTGCCTGGGTCGCAGGATTGCAGAACTGGAGATGCAA
  CTCCTGCTGTCAAGGCTGATACAAAAGTATGAGGTGGTCCTGTCTCCCGGGATGGGAGAAGTGAAGTCTG
  TGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTGAGCCTACGCTTTCTGCAGAGACAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178847
• ORF Size: 1602 bp
• Insert Size: 1602
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_178847.2, NP_849178.2
• RefSeq Size: 2150
• RefSeq ORF: 1602
• Locus ID: 301517
• Gene Summary: mitochondrial enzyme with both vitamin D3 25-hydroxylase and cholesterol 26-hydroxylase activities [RGD, Feb 2006]