Qtrt1 (NM_022250) Rat Tagged ORF Clone

CAT#: RR207670

Qtrt1 (Myc-DDK-tagged ORF) - Rat queuine tRNA-ribosyltransferase 1 (Qtrt1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Qtrt1
• Synonyms: Tgt; Tgut
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207670 representing NM_022250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTAGGCAGCCCAGGTTCGCTTGAGTCCGCTCCACGAATCATGCGGCTAGTCGCTGAGTGCA
  GTCGCTCCAGAGCTCGGGCCGGTGAGCTGCGGCTGCCGCATGGAACGGTAGCCACCCCTGTGTTCATGCC
  TGTGGGTACACAGGCCACCATGAAGGGTATCACCGCGGAGCAGCTGGACTCCCTGGGCTGCCGCATCTGC
  TTGGGCAACACCTACCATCTGGGGTTGAGGCCGGGTCCGGAGCTGATCCAGAAAGCTCACGGTCTTCACG
  GTTTCATGAATTGGCCCCACAATCTGCTGACGGATAGCGGCGGCTTCCAGATGGTGTCTCTCATCTCCTT
  GTCCGAGGTGACGGAGGAGGGCGTCCGCTTCCGCTCGCCCTATGATGGAGAAGAGACACTTTTGAGCCCA
  GAGAGGTCGGTGGAGATCCAGAACGCTCTGGGCTCGGACATCATCATGCAGTTGGACGATGTGGTGAGCA
  GCACGGTGACAGGCCCTCGAGTGGAGGAGGCCATGCACAGGTCAGTCCGCTGGCTGGACAGATGCATCGC
  AGCCCATAAGCGTCAGGACAAGCAGAACCTCTTTGCCATCATCCAGGGCGGACTGAATGCTGATCTTCGG
  ACCACTTGCCTGAAAGAGATGACCAAGAGGGATGTGCCGGGCTTTGCCATCGGAGGTCTGAGCGGAGGAG
  AGAGCAAGGAGCAGTTCTGGAAGATGGTGGCATTGAGCACCTCCATGCTGCCTAAGGACAAGCCACGCTA
  CCTGATGGGGGTTGGCTATGCCACCGATTTGGTGGTCTGCGTGGCCCTTGGATGTGACATGTTCGACTGT
  GTATACCCCACACGGACAGCGCGTTTTGGATCCGCCCTTGTGCCCACTGGGAACCTGCAGTTGAAGAAGC
  AGCAGTACGCCAAGGACTTCAGCCCCATAAACCCCGAGTGTCCCTGTGCCACCTGCCAGACGCACAGCCG
  CGCCTTCTTGCACACCCTGCTGCACAGTGACAACACCGCAGCGCTGCACCACCTCACCGTGCACAACATT
  GCCTATCAGCTGCAGCTCCTGAGCGCCGCGCGCAGCAGTATCTTGGAACAGCGCTTTCCCGACTTCGTGC
  GGAACTTCATGCGGACCATGTATGGGGACCACAGCCTCTGTCCTGCCTGGGCCATTGAAGCACTGGCCTC
  TGTGGGAATCACACTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207670 representing NM_022250
  Red=Cloning site Green=Tags(s)
  
  MAAVGSPGSLESAPRIMRLVAECSRSRARAGELRLPHGTVATPVFMPVGTQATMKGITAEQLDSLGCRIC
  LGNTYHLGLRPGPELIQKAHGLHGFMNWPHNLLTDSGGFQMVSLISLSEVTEEGVRFRSPYDGEETLLSP
  ERSVEIQNALGSDIIMQLDDVVSSTVTGPRVEEAMHRSVRWLDRCIAAHKRQDKQNLFAIIQGGLNADLR
  TTCLKEMTKRDVPGFAIGGLSGGESKEQFWKMVALSTSMLPKDKPRYLMGVGYATDLVVCVALGCDMFDC
  VYPTRTARFGSALVPTGNLQLKKQQYAKDFSPINPECPCATCQTHSRAFLHTLLHSDNTAALHHLTVHNI
  AYQLQLLSAARSSILEQRFPDFVRNFMRTMYGDHSLCPAWAIEALASVGITLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022250
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022250.2, NP_071586.2
• RefSeq Size: 1431
• RefSeq ORF: 1212
• Locus ID: 64016
• MW: 44.2 kDa
• Gene Summary: catalyzes exchange of queuine (the base of queuosine, Q) in tRNA queuosine biosynthesis [RGD, Feb 2006]