Bop1 (NM_001024250) Rat Tagged ORF Clone

CAT#: RR207789

Bop1 (Myc-DDK-tagged ORF) - Rat block of proliferation 1 (Bop1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bop1
• Synonyms: MGC109114
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207789 representing NM_001024250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGCGTGTGGTAAGCACCACATGTCACCGGGATCGCTGCCAGGGAAACGGCGTTTGGAACCTG
  ATCAGGAGCCACAGATACAAGAGCCTCTTCTCCTCAGCGACCCTGACTCCAGTCTCTCTGACAGCGAGGA
  GAGTGTGTTTTCAGGCCTTGAAGATTCTGGCAGTGACACGAGTGACGAGGACACTGAAGAAGTGGCCAGA
  GCTGGTTGTGACAAAGACAATAGAACAGAGAAGACCTCTGAGGAGCAGGAACAGGCTGTGCCCCCTTGCC
  CAAGGGCAGAGGAAGCAGGTGCTCTGACCAGAGATGAGTATGAAGAGGACAGCTCTGATGAGGAGGACAT
  TCGGAACACCGTGGGCAATGTGCCCCTGGCGTGGTATGACGATTTCCCACATGTGGGCTATGACCTGGAC
  GGCAAACGTATCTATAAGCCCCTGCGGACACGAGATGAGCTGGACCAGTTTCTGGACAAAATGGATGACC
  CAGATTTCTGGCGCACTGTGCAAGACAAGATGACAGGGAGTGATCTTCGGCTGACTGATGAGCAGGTTGC
  CCTGGTACATCGGCTTCAGAGAGGCCAGTTTGGAGATTCAGGCTTTGACCCCTATGAGCCAGCTGTGGAT
  TTCTTCAGTGGTGACATCATGATCCACCCCGTGACCAACCGCCCAGCTGACAAACGTAGTTTCATCCCGT
  CCCTAGTTGAGAAGGAGAAGGTGTCTCGATTGGTGCATGCCATCAAGATGGGTTGGATCAAGCCTCGACG
  GCCCCCCGACTCCACTCCTAGCTTCTATGACCTGTGGGCCCAGGAGGATCCAAATGCTGTGTTGGGGCGC
  CACAAGATGCACGTGCCTGCACCCAAGCTGGCCCTGCCCGGCCATGCGGAGTCTTACAACCCACCTCCCG
  AGTACCTGCCCACTGAGGAGGAGCGCTTGGCATGGATGCAGCAGGAGCCCATTGAGAGGAAGCTTAACTT
  CTTACCACAGAAGTTCCCCAGCCTGAGGACAGTGCCTGCTTATGGCCGCTTCATCCAGGAGCGTTTTGAA
  CGCTGTCTTGATTTGTATCTGTGCCCACGGCAGCGCAAGATGAGGGTGAATGTGGATCCCGAAGACCTCA
  TCCCCAAGCTCCCTCGGCCAAGAGACCTTCAGCCTTTCCCTGTGTGCCAGGCCCTTGTCTACAGGGGCCA
  CAGCGACCTTGTCCGTTGCCTCAGTGTCTCCCCAGGGGGCCAGTGGCTAGCTTCAGGTTCAGACGACGGC
  ACATTGAGGCTTTGGGAGGTGGCCACTGCCCGCTGCATGAAGACGGTGCGTGTTGGAGGTGTGATACGGA
  GCATTGCTTGGAACCCCAATCCCACCATATGCTTAGTAGCCGCTGCCATGGATGATGCCGTGGTGCTGCT
  GAACCCAGCCCTGGGGGACCGGCTGCTGGTGGGCAGCACAGACCAGCTGCTGGAAACCTTCACTCCCCCT
  GAAGAGCCAACTTTGCAGCCTGCCCACTGGCTGGAGGTCTCAGAGGAAGAACGCCAGAGGGGCCTTCGAC
  TACGTATCTGCCACAGTAAACCAGTGACACAGGTGACCTGGCATGGGCGAGGGGACTACCTGGCTGTGGT
  GCTGTCCAGCCAAGGGCACACTCAAGTGCTGATCCACCAGCTGAGCAGAAGGCGCAGCCAGAGCCCATTC
  CGCCGCAGCCACGGACAGGTGCAGTGTGTGGCTTTCCATCCCACGCGGCCCTTCCTGCTTGTGGCCTCCC
  AGCGCAGCATCCGCATTTACCACCTGCTACGCCAGGAGCTTACGAAGAAGCTGATGCCTAACTGCAAGTG
  GGTGTCTAGCATGGCTGTGCATCCAGCAGGTGACAACATCATCTGTGGCAGCTATGACAGCAAACTGGTG
  TGGTTTGACCTGGATCTTTCCACCAAGCCATACAAAGTGCTGAGGCACCACAAGAAGGCCTTGCGGGCTG
  TGGCCTTCCACCCCCGATACCCACTCTTTGCATCCGGCTCAGACGACGGCAGCGTTATCGTTTGCCATGG
  CATGGTGTACAATGACCTGCTGCAGAACCCATTGCTGGTGCCCGTCAAGGTGCTTAAGGGACACAGCCTG
  ACCCGAGATCTGGGTGTACTGGATGTGGCCTTCCACCCCACACAGCCATGGGTCTTCTCCTCCGGGGCAG
  ATGGCACCATTCGACTCTTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207789 representing NM_001024250
  Red=Cloning site Green=Tags(s)
  
  MAGACGKHHMSPGSLPGKRRLEPDQEPQIQEPLLLSDPDSSLSDSEESVFSGLEDSGSDTSDEDTEEVAR
  AGCDKDNRTEKTSEEQEQAVPPCPRAEEAGALTRDEYEEDSSDEEDIRNTVGNVPLAWYDDFPHVGYDLD
  GKRIYKPLRTRDELDQFLDKMDDPDFWRTVQDKMTGSDLRLTDEQVALVHRLQRGQFGDSGFDPYEPAVD
  FFSGDIMIHPVTNRPADKRSFIPSLVEKEKVSRLVHAIKMGWIKPRRPPDSTPSFYDLWAQEDPNAVLGR
  HKMHVPAPKLALPGHAESYNPPPEYLPTEEERLAWMQQEPIERKLNFLPQKFPSLRTVPAYGRFIQERFE
  RCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPVCQALVYRGHSDLVRCLSVSPGGQWLASGSDDG
  TLRLWEVATARCMKTVRVGGVIRSIAWNPNPTICLVAAAMDDAVVLLNPALGDRLLVGSTDQLLETFTPP
  EEPTLQPAHWLEVSEEERQRGLRLRICHSKPVTQVTWHGRGDYLAVVLSSQGHTQVLIHQLSRRRSQSPF
  RRSHGQVQCVAFHPTRPFLLVASQRSIRIYHLLRQELTKKLMPNCKWVSSMAVHPAGDNIICGSYDSKLV
  WFDLDLSTKPYKVLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVKVLKGHSL
  TRDLGVLDVAFHPTQPWVFSSGADGTIRLFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024250
• ORF Size: 2193 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024250.1, NP_001019421.1
• RefSeq Size: 2587 bp
• RefSeq ORF: 2196 bp
• Locus ID: 300050
• Cytogenetics: 7q34
• MW: 82.6 kDa