Samm50 (NM_001004241) Rat Tagged ORF Clone

CAT#: RR208059

Samm50 (Myc-DDK-tagged ORF) - Rat sorting and assembly machinery component 50 homolog (S. cerevisiae) (Samm50), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Samm50
• Synonyms: MGC94439
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208059 representing NM_001004241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGTGCACGCCAGGAGTCTGGAGCCTCTCCCATCAAGTGGAACTGACTTTGGAGCACTGGGGG
  AGGAAGCGGAGTTTGTGGAAGTTGAACCTGAAGCAAAACAGGAAATCCTGGAAAACAAAGATGTGGTGGT
  TCAGCATGTTCATTTTGATGGACTTGAACGGACCAAGGATGACATCATCATATGTGAAATCGGAGAGGTT
  TTTAAGGCTAAAAACCTCATTGAGGTAATGCGGCGGTCTCATGAGGCCCGTGAAAAACTGCTTCGCCTAG
  GAATTTTTAGACAAGTGGATGTTTTGATAGACACGTGTCATGGCGAAGATGCCCTGCCAAATGGGCTAGA
  TGTCACCTTTGAAGTTACAGAGCTAAGGAGACTGACGGGCAGTTACAACACCATGGTTGGAAACAACGAA
  GGCAGTATGGTACTCGGCCTCAAACTCCCCAATCTTCTGGGACGTGCAGAAAAAGTGACTTTCCAGTTTT
  CTTATGGAACCAAAGAAACTTCCTACGGCCTGTCTTTCTTCAAGCCACAGCCTGGGAACTTCGAGAAAAA
  CTTCTCGGTAAACTTATATAAAGTTACCGGGCAGTTCCCATGGAGCTCACTTCGGGAGACGGACAGAGGA
  GTGTCTGCTGAGTACAGTTTTCCACTATGCAAGACCAGTCACACTGTCAAGTGGGAGGGTGTGTGGCGGG
  AACTGGGCTGCCTCGCGCGGACTGCGTCATTCGCTGTGCGGAAGGAAAGTGGACACTCATTGAAGTCGTC
  TCTCTCGCATGCCATGGTCATTGACTCTCGGAATTCATCTATCTTGCCAAGAAGAGGGGCCTTGCTCAAA
  GTCAACCAGGAGCTGGCAGGCTACACTGGAGGAGATGTGAGCTTCATCAAGGAAGACTTTGAGCTTCAGC
  TGAATAAGCCGCTTGTATTGGATTCGGTGTTCTCCACGTCCCTCTGGGGTGGAATGCTGGTGCCCATCGG
  TGACAAGCTATCCAGCATTGCTGACAGGTTTTACCTGGGAGGCCCCACGAGCGTCCGAGGATTTAGCATG
  CACAGCATCGGACCCCAGAGCGAAGGAGATTACCTGGGCGGGGAGGCATACTGGGCCGGGGGCCTGCACC
  TCTATACCCCACTGCCTTTCCGGCCAGGCCAGGGTGGCTTCGGAGAGCTTTTCAGAACTCACTTTTTCCT
  CAATGCTGGCAACCTGTGCAACCTCAACTACGGTGAGGGCCCCAGAGCCCACATCCGGAAGCTGGCTGAA
  TGCATCCGCTGGTCCTATGGTGCAGGCATCGTCCTCCGGCTTGGCAACATTGCTCGGCTGGAGCTGAACT
  ACTGCATTCCTATGGGTGTGCAGCGAGGCGACAGGATTTGTGATGGCGTCCAGTTTGGAGCTGGGATTCG
  GTTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208059 representing NM_001004241
  Red=Cloning site Green=Tags(s)
  
  MGTVHARSLEPLPSSGTDFGALGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLERTKDDIIICEIGEV
  FKAKNLIEVMRRSHEAREKLLRLGIFRQVDVLIDTCHGEDALPNGLDVTFEVTELRRLTGSYNTMVGNNE
  GSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPQPGNFEKNFSVNLYKVTGQFPWSSLRETDRG
  VSAEYSFPLCKTSHTVKWEGVWRELGCLARTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLK
  VNQELAGYTGGDVSFIKEDFELQLNKPLVLDSVFSTSLWGGMLVPIGDKLSSIADRFYLGGPTSVRGFSM
  HSIGPQSEGDYLGGEAYWAGGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPRAHIRKLAE
  CIRWSYGAGIVLRLGNIARLELNYCIPMGVQRGDRICDGVQFGAGIRFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004241
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004241.1, NP_001004241.1
• RefSeq Size: 1638 bp
• RefSeq ORF: 1410 bp
• Locus ID: 300111
• Cytogenetics: 7q34
• MW: 52 kDa