Neil1 (NM_001025754) Rat Tagged ORF Clone

CAT#: RR208819

Neil1 (Myc-DDK-tagged ORF) - Rat nei endonuclease VIII-like 1 (E. coli) (Neil1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neil1
• Synonyms: MGC116267
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208819 representing NM_001025754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGGGCCCAGAGCTGCACCTGGCCAGCCACTTTGTGAATGAGATATGTAAAGGGCTGGTATTCG
  GTGGGTGTGTGGAGAAGTCCTCTGTCAGCCGAAACCCGGAGGTGCCCTTTGAGAGCAGTGCCTACCACAT
  CTCAGCTTTAGCCCGAGGCAAGGAGCTGCGCTTGACATTGAGCCCCCTGCCTGGGTCCCATCCCCCTCAG
  AAACCACTGTCCCTTGTCTTCCGCTTTGGTATGTCTGGATCCTTCCAGCTGGTACCCGCAGAAGCACTGC
  CGCGCCACGCCCATCTACGTTTCTATACGGCCCCACCTGCCCCCCGGCTTGCCCTTTGCTTCGTAGACAT
  CCGCCGCTTTGGCCACTGGGACCCTGGAGGTGAATGGCAGCCAGGCCGCGGACCCTGTGTCTTGTTGGAG
  TATGAACGGTTCAGAGAGAACGTGCTTCGGAACCTATCGGACAAAGCCTTTGACCGGCCCATCTGCGAGG
  CCTTGTTGGACCAGAGGTTCTTCAATGGAATCGGCAACTATCTGCGGGCAGAGATCTTGTATCGGCTGAA
  GATCCCCCCTTTTGAGAAGGCCCGTACAGTTCTAGAGGCCCTGCAACAGTGCCGGCTGAGCCCAGAGCTG
  ACCCTGAGCCAGAAGGTCAAGGCCAAACTGCAGAACCCAGACCTGCTGGAACTGTGTCACTTGGTGCCCA
  AGGAAGTGGTTCAGCTGGGGGGCAAAGGCTATGGGCCAGAGCGTGGAGAGGAAGATTTTGCTGCCTTTCG
  AGCCTGGCTTCGGTGCTATGCTGTGCCAGGCATGAGCTCCCTGAGAGACCGGCATGGCCGTACCATTTGG
  TTCCAGGGTGATCCTGGACCCTTGGCACCCAAAGGGGGCAGATCCCGAAAAAAGAAGTCACAGGAAACAC
  AGCTGGAGGCTGAGGACAGAAAAGAGGACCCTCCAAGCAAGTCCATTTCCAGGACTCAGAGGGCCAGGAA
  GCACTGTCCTAAGAATGTACCTCAACAGTCTGAAGGGGCCAGCCTCCCACAAAACCTGGAAACCCCTGCA
  GTCCCTGAGAAAGGAAAGAGAAGGGGGAGACGGGCGAGCACAGGCCGCCACAGAGGACCAAAGAGTAAGC
  CTGATACTCAACCCAGGGAGGCTGGGGAGATTTCAGCCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208819 representing NM_001025754
  Red=Cloning site Green=Tags(s)
  
  MPEGPELHLASHFVNEICKGLVFGGCVEKSSVSRNPEVPFESSAYHISALARGKELRLTLSPLPGSHPPQ
  KPLSLVFRFGMSGSFQLVPAEALPRHAHLRFYTAPPAPRLALCFVDIRRFGHWDPGGEWQPGRGPCVLLE
  YERFRENVLRNLSDKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARTVLEALQQCRLSPEL
  TLSQKVKAKLQNPDLLELCHLVPKEVVQLGGKGYGPERGEEDFAAFRAWLRCYAVPGMSSLRDRHGRTIW
  FQGDPGPLAPKGGRSRKKKSQETQLEAEDRKEDPPSKSISRTQRARKHCPKNVPQQSEGASLPQNLETPA
  VPEKGKRRGRRASTGRHRGPKSKPDTQPREAGEISAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025754
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025754.1, NP_001020925.1
• RefSeq Size: 1768 bp
• RefSeq ORF: 1164 bp
• Locus ID: 367090
• Cytogenetics: 8q24
• MW: 43.3 kDa