Serpinf2 (NM_001011892) Rat Tagged ORF Clone

CAT#: RR209003

Serpinf2 (Myc-DDK-tagged ORF) - Rat serine (or cysteine) peptidase inhibitor, clade F, member 2 (Serpinf2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Serpinf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209003 representing NM_001011892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGCTCCGGGGGCTCCTGGTACTTAGCTTGTCCTGTCTGCAAGGTCCCAGTTCAATGTTCTCTC
  CTGTGAGTGCCATGGATCTCCCGGGCCAGCAGCCAGTGAGTGAGCAAGCCCAGCAGAAGCTGCCCCCACT
  TGCCCTCCTCAAGTTGGGCAACCAGGATCTTGGTGACCATGCTACCCTGAAGAGGTCCCCAGGAGATTGC
  AAGAGTGCCCCAACTACAGAGGAGACTCGCAGGCTGTCTCAGGCCATGATGGCTTTTACTACTGACCTGT
  TCTCCCTGGTGGCCCAAACATCCACCAGCTCCAACCTTGTCCTGTCACCCCTTAGTGTGGCCCTAGCACT
  CTCTCACCTGGCACTAGGTGCTCGGAACCAAACACTGGAGAACCTGCAACGAGTGCTGCACATGAACATG
  GGATCCTGCATCCCGCATCTACTGAGCCATTTCTGCCAGAACCTAAACCCCGGGACAATCCGACTGGCGG
  CCAGAATATACTTGCAGAAAGGATTTCCCATCAAAGATGATTTCCTGGAGCAATCGGAAAAGCTCTTTGG
  CGCGAAGCCCGTGAAACTGACTGGAAGGCAGGAAGAAGACCTGATGAACATCAACAAATGGGTGAAGGAG
  GCCACAGAAGGGAAGATTGAGGATTTCCTCTCTGAGCTGCCGGATAACACCGTGCTGCTCCTCCTCAATG
  CCATCCACTTTCACGGTTTCTGGAGGACCAAGTTTGACCCCAGCCTCACCCAGAAAGACTCCTTCCACCT
  GGACGAGCAGTTCACGGTGCCAGTGGCCATGATGCACGCACAGTCATACCCCCTTCGATGGTTCCTGCTG
  GAGCAACCTGAGATACAGGTGGCTCATTTCCCATTTCAGAACAACATGAGCTTCGTGGTCATAATGCCCA
  CTTACTTTGGGTGGAACGTGTCCGAGGTACTAGCCAACCTGACCTGGGACACTCTGTACCAGCCCTCGAT
  GCGGGAGAAGCCCACCAAGGTGCGGCTGCCTAAACTTCATCTGGAACAGCATCTGGACCTGGTGGCCACC
  CTCAGCAAGCTGGGCCTACAGGACTTGTTCCAGAGCCCAGACCTGCGTGGGATCTCTGACCAGAGTCTGG
  TGGTGTCTAGTGTGCAGCACCAGTCTACCATGGAGCTCAGTGAGGCTGGTGTGGAGGCAGCGGCAGCTAC
  CAGCACAGCCATGACTCGAATGTCCCTTTCCTCCTTCTTTTTGAACCGACCATTCATCTTCTTCATCATG
  GAGGAGACCATAGGCATACCCCTCTTTGTGGGAAGTGTGAGAAACCCTGACCCCAGTGCACAGCCCCAGC
  CCCAGGAACAGCAAGACTCCCCTGACAACAGGCGCTTAGACCAGAATGACAAAGCTGACATCCCCGGAGG
  CAAGACCTTTGCTCCTGACTTGAAACTTGTGCCCCGCCTGGAGGAAGATTATCCCCAGTTTAGCTCCCCC
  AAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209003 representing NM_001011892
  Red=Cloning site Green=Tags(s)
  
  MALLRGLLVLSLSCLQGPSSMFSPVSAMDLPGQQPVSEQAQQKLPPLALLKLGNQDLGDHATLKRSPGDC
  KSAPTTEETRRLSQAMMAFTTDLFSLVAQTSTSSNLVLSPLSVALALSHLALGARNQTLENLQRVLHMNM
  GSCIPHLLSHFCQNLNPGTIRLAARIYLQKGFPIKDDFLEQSEKLFGAKPVKLTGRQEEDLMNINKWVKE
  ATEGKIEDFLSELPDNTVLLLLNAIHFHGFWRTKFDPSLTQKDSFHLDEQFTVPVAMMHAQSYPLRWFLL
  EQPEIQVAHFPFQNNMSFVVIMPTYFGWNVSEVLANLTWDTLYQPSMREKPTKVRLPKLHLEQHLDLVAT
  LSKLGLQDLFQSPDLRGISDQSLVVSSVQHQSTMELSEAGVEAAAATSTAMTRMSLSSFFLNRPFIFFIM
  EETIGIPLFVGSVRNPDPSAQPQPQEQQDSPDNRRLDQNDKADIPGGKTFAPDLKLVPRLEEDYPQFSSP
  K
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011892
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011892.1, NP_001011892.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1476 bp
• Locus ID: 287527
• Cytogenetics: 10q24
• MW: 54.9 kDa