Atp5j (NM_053602) Rat Tagged ORF Clone

CAT#: RR209022

Atp5j (Myc-DDK-tagged ORF) - Rat ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (Atp5j), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp5j
• Synonyms: Atp5j
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209022 representing NM_053602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTTCAGAGGATCTTCAGGCTCTCCTCTGTCCTTCGGTCAGCAGTCTCTGTGCATTTGAGGAGGA
  ACATTGGTGTTACAGCTGTGGCGTTTAATAAGGAACTTGATCCTGTACAGAAACTCTTCTTGGACAAGAT
  AAGAGAGTACAAAGCAAAGCGACTGGCGTCTGGAGGACCTGTTGATACTGGCCCAGAATATCAGCAAGAG
  GTGGACAGAGAGCTTTTTAAGCTTAAACAAATGTATGGTAAAGGAGAGATGGATAAGTTTCCTACCTTCA
  ATTTTGAGGATCCCAAATTTGAAGTCCTCGACAAACCCCAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209022 representing NM_053602
  Red=Cloning site Green=Tags(s)
  
  MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQE
  VDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053602
• ORF Size: 324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053602.1, NM_053602.2, NP_446054.1
• RefSeq Size: 571
• RefSeq ORF: 327
• Locus ID: 94271
• MW: 12.5 kDa
• Gene Summary: coupling factor 6 of the mitochondrial ATP synthase complex [RGD, Feb 2006]