Cep55 (NM_001025646) Rat Tagged ORF Clone

CAT#: RR209096

Cep55 (Myc-DDK-tagged ORF) - Rat centrosomal protein 55kDa (Cep55), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cep55
• Synonyms: RGD1305340
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209096 representing NM_001025646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAGAAGTCCCAAAGATTTAATTAAAAGCAAATGGGGATCGAGGCCTAGCAGCTCCAAGTCAG
  ACACTGCGCTAGAGAAATTTAAGGGAGAAATTGCAGCCTTCAAAACATCCCTGGATGAAATCACCAATGG
  GAAAGGAAAAGTGGCCAATAAAGACAGAAGCAAACTTCTGGAGAAAATTCAAGTTCTTGAAGCCGAGAGA
  GAAAAGAATGTTTATTACCTCATGGAGAAGGACAAAGAAATACAGAGACTCAAAGACCACCTAAGGTCAA
  GATATAGCTCTTCGTCATTGCTCGAGCAGCTGGAGGAGAAAACCAAGGAGTGTGAGAAGAAGCAGCAGTT
  GCTGGAGTCCTTATCCAGAGAGACAGATATCCTGAAAAAGCAGTTGTCTGCCACGACGAAAAGACTTTCC
  GAACTTGAAAGCAAAGCCAGTACACTCCACCTATCGCAGAGCGTGCCTGCAAACTGCTTCAACTCATCAA
  TGAATAGTATTCATGAAAAGGAAATGCAGCTGAAAGATGCTCTGGAGAAAAATCAGCAGTGGCTTGTGTA
  CGACCAGCAGCGAGAAGCCTACGTTAAAGGGCTTTTAGCAAAGATCTTTGAGCTTGAAAAGAGAACAGAG
  ACTGCCGCTGCTTCCCTCCCACAGCAGATGAAAAAGACTGAATCAGAAGGTTATCTTCAAGAGGAGAAGC
  AAAAATATGATCATCTCTTGGAAAATGCCAAAAAAGATCTTGAAGTTGAGCGACAAACCGTAACTCAGTT
  GCGTTTAGAACTGGATGAATTTCGAAGGAAATATGAAGAAACTCAAAAAGAAGTTGAAGATTTAAACCAG
  CTGTTGAGTTCTCAGCGCAAGGCAGACATACAGCACCTGGAAGAGGACAAGCATAAGACGGAGAAAATCC
  AGAAGCTCAGGGAAGAGAGCAGTGTCTTCAAGGGGAAACTGGAAGAGGAGAGGAAGAAGTCTGAAGAGCT
  TTTATCTCAGGTCCGAATTCTCTACGATTCTCTGTTAAAGCACCAAGAGGAGCAGAGCAGGGTGGCTCTG
  CTGGAACGGCAGATGCAGGCATGTACTCTAGACTTTGAAAATGAAAAACTCGACCGCCAGAATATGCAGC
  ATCAGCTGTATGTGATTCTTAAGGAGCTCCGGAAAGCAAAAAGTCAAATAACACAGTTGGAATCCTTGAA
  GCAGCTACATGGATTTACCTTCACAGAGCAGCCGTTCCCACTCCAGGGAGAGCCTGAAAACAGAGTGAAA
  GCTACCTCACCTAAGAGTCCCACTGCTGTTCTCAACGAAAGCCTAGTGGAATGTCCCAAGTGCAGCGTAC
  AGTACCCAGCCACCGAACATCGAGACCTGCTTGTCCATGTGGAATACTGCATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209096 representing NM_001025646
  Red=Cloning site Green=Tags(s)
  
  MSSRSPKDLIKSKWGSRPSSSKSDTALEKFKGEIAAFKTSLDEITNGKGKVANKDRSKLLEKIQVLEAER
  EKNVYYLMEKDKEIQRLKDHLRSRYSSSSLLEQLEEKTKECEKKQQLLESLSRETDILKKQLSATTKRLS
  ELESKASTLHLSQSVPANCFNSSMNSIHEKEMQLKDALEKNQQWLVYDQQREAYVKGLLAKIFELEKRTE
  TAAASLPQQMKKTESEGYLQEEKQKYDHLLENAKKDLEVERQTVTQLRLELDEFRRKYEETQKEVEDLNQ
  LLSSQRKADIQHLEEDKHKTEKIQKLREESSVFKGKLEEERKKSEELLSQVRILYDSLLKHQEEQSRVAL
  LERQMQACTLDFENEKLDRQNMQHQLYVILKELRKAKSQITQLESLKQLHGFTFTEQPFPLQGEPENRVK
  ATSPKSPTAVLNESLVECPKCSVQYPATEHRDLLVHVEYCMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025646
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025646.1, NP_001020817.1
• RefSeq Size: 2481 bp
• RefSeq ORF: 1389 bp
• Locus ID: 294074
• Cytogenetics: 1q53
• MW: 54 kDa