Galnt14 (NM_001012109) Rat Tagged ORF Clone

CAT#: RR209138

Galnt14 (Myc-DDK-tagged ORF) - Rat UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14) (Galnt14), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galnt14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209138 representing NM_001012109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGCTGACTCGCCGCTTGGCGCTGCCCATCTTTGGGGTGCTTTGGATCACCGTGCTGCTGTTCT
  TCTGGGTCACCAAGAGGAAGTTGGAGGTGCCGCTGGGACCCGAAGTGCAGACCCCCAAGCCTTCTGATGC
  GGACTGGGATGACCTGTGGGAGCAGTTTGATGAGCGGCGGTATCTGAATGCCAAAAAGTGGCGTGTCGGT
  GATGATCCCTACAAGCTGTACGCTTTCAACCAGCGGGAGAGCGAGCGGATATCCAGCAATCGGGCTGTCC
  CAGACACACGCCATAAGAGATGTTCACTGCTTGTGTACTGCACGGACCTGCCTCCCACCAGCATCATCAT
  CACCTTCCACAACGAGGCACGTTCCACTCTGCTCAGAACTATCCGCAGTGTGTTAAACCGCACCCCTATG
  CATCTGATCCAGGAAATCATATTAGTGGATGACTTCAGCAATGACCCTGAGGACTGCAAACAGCTCATCA
  AACTGCCCAAGGTGAAGTGCTTACGCAACAGTGAGCGGCAAGGTCTGGTCCGGTCCCGGATGCGAGGTGC
  TGACATTGCCCAAGGCACCACTCTGACCTTCTTGGACAGCCACTGTGAGGTGAACAGGGATTGGCTGCAG
  CCCTTGTTACACAGGGTCAAAGAGGATTACACAAGGGTAGTGTGCCCTGTGATCGACATCATTAACCTGG
  ACACCTTCAACTATATTGAGTCTGCCTCAGAGCTCAGAGGGGGGTTTGACTGGAGCCTGCACTTCCAGTG
  GGAGCAGCTCTCCGTAGAACAGAAAGCTTTGCGCCTAGACCCGACTGAGCCCATCCGGACTCCAATCATA
  GCTGGAGGGCTCTTCGTGATTGACAAAGCCTGGTTCGATTACCTGGGTAAATATGACGTGGACATGGACA
  TCTGGGGTGGGGAGAACTTTGAAATCTCTTTCCGAGTGTGGATGTGCGGAGGTGGCCTGGAGATCATCCC
  CTGCAGTCGGGTGGGGCATGTCTTCAGGAAAAAGCATCCATATGTCTTCCCTGATGGCAACGCCAACACA
  TACATAAAGAACACCAAGCGGACAGCTGAAGTATGGATGGATGAATACAAGCAATACTACTATGCAGCCC
  GGCCTTTTGCCCTGGAGAGACCCTTTGGAAACATTGAGAACAGGCTGAACCTGAGGAAGAATCTGCACTG
  TCAGACCTTCAAGTGGTACCTGGAAAATGTCTACCCAGAACTCAGGGTCCCCCCAGACTCCTCCATCCAG
  AAGGGCAATATCCGACAGCGGCAGAAGTGCCTTGAGTCTCAAAAGCAGAAAAACCAAGAGACTCCACACC
  TCAGGCTAAGCCCCTGTGCCAAGGTCAAAGGAGATAGAGCAAAGTCTCAGGTGTGGGCCTTCACATACAC
  CCAACAGATCATCCAGGAAGAGCTGTGCCTGTCTGTGGTCACCCTGTTTCCTGGTGCCCCAGTAGTTCTT
  GTTCTATGCAAGAATGGGGATGAAAGACAGCAATGGACCAAGACCGGTGCTCGCATTGAGCACATAGCAT
  CCCACCTCTGCCTGGACACGGACATGTTCGGTGACAGCACCGAGGATGGCAAGGAGGTCGTTGTCAACCC
  TTGTGAGTCGTCACTCATGAGCCAGCACTGGGACATTGTGAGCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209138 representing NM_001012109
  Red=Cloning site Green=Tags(s)
  
  MRRLTRRLALPIFGVLWITVLLFFWVTKRKLEVPLGPEVQTPKPSDADWDDLWEQFDERRYLNAKKWRVG
  DDPYKLYAFNQRESERISSNRAVPDTRHKRCSLLVYCTDLPPTSIIITFHNEARSTLLRTIRSVLNRTPM
  HLIQEIILVDDFSNDPEDCKQLIKLPKVKCLRNSERQGLVRSRMRGADIAQGTTLTFLDSHCEVNRDWLQ
  PLLHRVKEDYTRVVCPVIDIINLDTFNYIESASELRGGFDWSLHFQWEQLSVEQKALRLDPTEPIRTPII
  AGGLFVIDKAWFDYLGKYDVDMDIWGGENFEISFRVWMCGGGLEIIPCSRVGHVFRKKHPYVFPDGNANT
  YIKNTKRTAEVWMDEYKQYYYAARPFALERPFGNIENRLNLRKNLHCQTFKWYLENVYPELRVPPDSSIQ
  KGNIRQRQKCLESQKQKNQETPHLRLSPCAKVKGDRAKSQVWAFTYTQQIIQEELCLSVVTLFPGAPVVL
  VLCKNGDERQQWTKTGARIEHIASHLCLDTDMFGDSTEDGKEVVVNPCESSLMSQHWDIVSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012109
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012109.1, NP_001012109.1
• RefSeq Size: 2310 bp
• RefSeq ORF: 1659 bp
• Locus ID: 313878
• Cytogenetics: 6q13
• MW: 64.3 kDa