Arhgef1 (NM_021694) Rat Tagged ORF Clone

CAT#: RR209197

Arhgef1 (Myc-DDK-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef1
• Synonyms: Lbcl2; Lsc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209197 representing NM_021694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAGTCGCCGGAGGGGCGGCCCCAGGGCCTCCCCGGTCTGGCCTGGTGTCCATCATCATCGGGG
  CTGAGGATGAGGACTTTGAGAATGAACTGGAGGCGAACCCAGAAGATCAAAACAGCCAGTTCCAGAGCCT
  GGAGCAAGTGAAGCGTCGCCCAGCCCACCTCATGGCCCTCCTGCAACACGTGGCCCTGCAGTTCGAGCCA
  GGACCACTGCTCTGCTGCCTGCATGCTGACATGCTAAGCTCACTGGGCCCCAAAGAAGCCAAGAAGGCCT
  TCCTCGATTTCTATCACAGTTTCCTGGAGAAGACTGCGGTTCTGCGGGTGCCGGTCCCTCCCAGCGTTGC
  TTTTGAACTTGATCGTACTCGGCCTGATCTCATCTCTGAGGATGTCCAGAGGCGGTTCATACAAGAGGTG
  GTACAGAGCCAGCAGGCAGCCGTGACCCGTCAGCTAGAGGACTTCCGCTCCAAACGGCTCATGGGCATGA
  CACCCTGGGAGCAGGAACTGAGCCTGCTAGAGCCCTGGATTGGGAAAGACCGGGGCAACTATGAGGCCCG
  GGAGCGGCATGTTGCAGAGCGGCTGCTCTCCCACCTAGAGGAAATGCAACATACCATCTCCACAGATGAA
  GAAAAAAGTGCTGCTGTGGTCACTGCAATCAGCCTGTATATGCGCCACCTTGGGGTCCGGACCAAGAGTG
  GGGACAAGAAGTCAGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAATCGGCGGTCAGACGAGCCCCC
  CAAGACAAAGAAAGGGCTGAGCAGTATCCTAGATCCTGCCCGCTGGAACCGGGGAGAGCCATCTGCTCCA
  GACTGTCGACATCTAAAGGTCGAGGTTGATGAGAAGCCAGGCCCTGCAGACCGGAAGGGAAGCCTGGGTA
  TTTCATCTCGGGACAGGACTGTTGGGACTCCTGGGCAGGACAACCCAGGAGTCTCCCTGCACCCTCTGTC
  TGTAGACAGCCTGGATTCCCGGGAACCAGGTGTGGATACCCCGCAGGAGCCAGGAGATACACCCCCACAA
  GGCCCTACAAGCCTGGAGCCCCTGGCACCCCCAGAGAGCACAGAGGACAATGGCGAGACTGAAAGCCCTG
  AGCCTGGAGATGATGGAGAGCCAGGGCGGTCAGGCCTGGAACAGGAACCAGAAGAACCTCCTGGGTGGAG
  GGAACTGGTGCCCTCAGACACCCTGCTCGGCCTGCCCAAGAACCAAGTGAAGCGGCAAGAGGTCATCAGT
  GAGCTGCTGGTGACGGAGGCGGCTCACGTCCGCATGCTACGGGTACTACATGACCTCTTCTACCAGCCCA
  TGGCAGAAGGGGGCTTCTTCCCTCTGGAGGAACTGCAGAACATCTTTCCCAGCCTGGATGAGCTCATCGA
  GGTGCACTCCCTGTTCCTCGATCGCTTGATGAAGCGGAGACAAGAGAGTGGCTACCTCATTGAGGAGATT
  GGTGATGTGCTACTGGCCCGGTTCGATGGTGCTGAGGGCTCATGGTTCCAGAAGATCTCTTCCCGCTTCT
  GCAGCCGCCAGTCATTCGCTCTAGAGCAGCTCAAAGCCAAGCAGCGCAAGGAGCCTCGATTCTGTGCCTT
  TGTGCAGGAAGCTGAGAGCCGCCCCCGCTGCCGGCGGCTGCAGTTGAAGGACATGATCCCCACTGAGATG
  CAGCGGCTGACCAAGTACCCACTGCTGCTGCAGAGTATTGGGCAGAACACAGAAGAGCCTGCCGAGCGGG
  CAAAGGTGGAACTTGCAGCTGAGTGCTGCCGTGAGATTCTGCACCACGTTAATCAAGCTGTCCGTGACAT
  GGAGGACCTGCTGCGGCTCAAGGATTACCAGCGGCGCCTGGACTTGACTCACCTGCGGCAGAGCAATGAC
  CCAATGCTGAGCGAGTTCAAGAACCTGGATATCACTAAGAAGAAGTTGGTCCATGAAGGCCCCCTCACGT
  GGCGACTAACCAAAGACAAAGCTGTAGAGGTCCACGTGCTATTGCTGGACGACCTGCTGCTGCTGCTCCA
  GCGCCAGGATGAGAGGCTGCTGCTCAAGTCACACAGCCGGACGCTGACACCCACACCCGACGGCAAGACC
  ATGCTGCGGCCTGTGCTCCGGCTCACCTCTGCCATGACCCGGGAGGTGGCCACTGATCACAAAGCTTTTT
  ACGTCATTTTTACCTGGGACCAGGAGGCCCAGATATATGAGCTGGTGGCACAGACATCGTCGGAACGCAA
  GAGCTGGTGTGCCCTCATCACAGAGACTGCCGGATCCCTGAAGGTCCCTGCCCCTGCCTCCCGCCCCAAA
  CCCCGGCCCAGCCCAAGCAGCACCCGAGAACCCCTGCTCAGCAGCTCTGAGAATGGCACTGGAGGCACAG
  AGGCCGCTCCAGCTGATGCCCGGACAGAGCGGATCCTCAACGACCTCCTGCCCTTCTGCAGACCAGGCCC
  AGAGGGCCAGCTTGCTGCCACAGCCCTCCAGAAAGTACTGTCCCTGAAACAGATCCTGTTGAGCACTGAG
  GAAGACAGTGGAGCAGGGCCTCCCCGCGATGGGGATGGGGTGCCTGGTGGTGGTGCCCCAGGCCCAACAC
  ACACCCAGGAGGTTGAGGAAAACCTGTTGAGCCTAGAAGTGGTCATCAAACAACTGGAGGAGTTAGAAGA
  GGAATTTTGTCGCCTAAGACCCTTCCTGTCTCAGCTTGGGGAGATTCTGTCCCCCAACCTGGCTGCACCT
  GAGCGCTCTGCTCAGACAGGCCTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209197 representing NM_021694
  Red=Cloning site Green=Tags(s)
  
  MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANPEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEP
  GPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEV
  VQSQQAAVTRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEEMQHTISTDE
  EKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAP
  DCRHLKVEVDEKPGPADRKGSLGISSRDRTVGTPGQDNPGVSLHPLSVDSLDSREPGVDTPQEPGDTPPQ
  GPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLEQEPEEPPGWRELVPSDTLLGLPKNQVKRQEVIS
  ELLVTEAAHVRMLRVLHDLFYQPMAEGGFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEI
  GDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEM
  QRLTKYPLLLQSIGQNTEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSND
  PMLSEFKNLDITKKKLVHEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKT
  MLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKSWCALITETAGSLKVPAPASRPK
  PRPSPSSTREPLLSSSENGTGGTEAAPADARTERILNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTE
  EDSGAGPPRDGDGVPGGGAPGPTHTQEVEENLLSLEVVIKQLEELEEEFCRLRPFLSQLGEILSPNLAAP
  ERSAQTGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021694
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021694.1, NM_021694.3, NP_067726.3
• RefSeq Size: 3243
• RefSeq ORF: 2760
• Locus ID: 60323
• MW: 102.8 kDa
• Gene Summary: mouse homolog is an oncogene with similarity to the Dbl family of guanine nucleotide exchange factors [RGD, Feb 2006]