Pde3b (NM_017229) Rat Tagged ORF Clone

CAT#: RR209284

Pde3b (Myc-DDK-tagged ORF) - Rat phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209284 representing NM_017229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGGACGAGCGCGAGCGGGACACGCCAGCCATGAGGTCCCCGCCGCCGCCGCCGCCGCCGGCCA
  CGGCCACGGCCGCCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCG
  GCAGGACCCTCCGCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCG
  CTCCGCGCCGGGGCACGCCTCTCGCTCGCCGCTCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGG
  GGCCCGAGCGCTGGGCGGCCGCGGCGACCGGGCTTCGGACGCTGCTGAGCGCTTGCTCGCTCAGCCTCAG
  CCCGCTCTTCAGCATCGCCTGTGCCTTCTTCTTCCTCACCTGCTTCCTGACGCGCGCGCAGCGCGGCCCG
  GACCGCGGCGCCGGCTCCTGGTGGCTGCTGGCGCTGCCTGCCTGCTGCTACCTGGGCGACTTCGCGGCGT
  GGCAGTGGTGGTCGTGGCTGCGCGGGGAGCCGGCGGCGGCGGCGGCCGGCCGGCTCTGCCTGGTGCTGAG
  CTGCGTGGGGCTGCTGACGCTCGCGCCTCGCGTGAGGCTGCGGCACGGAGTCCTGGTGCTGCTCTTCGCC
  GGCCTGGTGTGGTGGGTGTCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGT
  GCCTGGTCGGGGGCGCGGGATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTC
  ACCTCCCCCGCGCTCGGCGAGTACCGCGGATGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGC
  TGCGTGTCGCTGGGAGAAAGCGCGGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGC
  CTTGTATTTCTCGAGAACAGATGATTCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCA
  GAATTCTGGAGGTGGAAATGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTT
  CTGATTGACCCAAGCCTTCCCCCACAAGTCATTTCTTCTTTGCGGAGTATCAGTAGCTTGATGGGTGCTT
  TCTCAGGTTCCTGTAGGCCAAAGATTAATTCTTTCACACCATTTCCTGGATTTTATCCCTGTTCTGAAGT
  AGAAGATCCAGTAGAGAAAGGCGATCGAAAACTTCACAAGGGGTTGAGTAGCAAACCCAGTTTCCCGACT
  GCACAGCTGAGGAGGAGCTCAGGAGCGTCAGGATTGCTGACCAGTGAGCACCACTCACGGTGGGATCGCA
  GCGGTGGCAAAAGGCCTTACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAG
  TTCAAATCTTATGACAATTCCAAAGCAGAGGTCATCATCTGTGTCGCTGACGCACCATGCAGGTCTGAGA
  AGAGCTGGTGCTTTGCCCAGCCCCAGTCTTCTGAATTCTTCAAGCCATGTACCCGTGTCTGCTGGCTGTC
  TAACTAATCGATCACCCGTAGGATTTCTTGATACCAGTGATTTTCTTACTAAGCCAAGTGTTACTTTACA
  TAGATCACTGGGCAGTGTATCCAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAGTCTG
  TGTAGCAGCTGTGGACACCAAATACTCAAGTATGTTTCGACATGTGAACCTGATGGTACAGACCACCACA
  ATGAAAAATCAGGTGAAGAAGACAGCACTGTTTTCTCAAAAGAACGATTGAACATTGTGGAAACTCAAGA
  AGAAGAGACAGTGAAGGAAGACTGCAGGGAATTATTTTTGGAAGGTGATGATCACCTAATGGAAGAGGCC
  CAGCAACCGAATATTGACCAGGAAGTGTTATTGGACCCGATGTTAGTGGAAGATTATGATTCATTAATAG
  AAAAGATGAGCAACTGGAACTTTCAGATTTTTGAGCTTGTAGAAAAAATGGGAGAAAAATCAGGAAGGAT
  TCTCAGTCAGGTTATGTATACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAA
  GAATTTATGAATTATTTTCGTGCACTAGAAAATGGCTACCGAGATATTCCATATCACAATCGTGTGCATG
  CCACAGATGTCCTACATGCTGTTTGGTATTTGACAACACGACCAATTCCTGGCTTACAGCAGCTCCATAA
  TAACCATGAAACAGAAACCAAAGCAGATTCAGATGCTAGACTTAGTTCTGGACAGATTGCTTACCTTTCT
  TCGAAGAGTTGCTGTATTCCAGATAAGAGTTATGGCTGCCTGTCTTCAAACATTCCTGCGTTAGAACTGA
  TGGCTTTATATGTGGCAGCTGCCATGCACGATTATGATCACCCAGGAAGAACAAATGCATTCCTAGTGGC
  TACAAATGCACCTCAGGCAGTTTTATACAATGACAGATCTGTTCTAGAAAATCATCATGCCGCATCAGCG
  TGGAATCTGTATCTTTCTCGCCCAGAATACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGTT
  TTCGATTTTTAGTCATAGAAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCTGAATT
  CAATGCCAAGGCCAATGATGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGACTCTTAGTC
  TGCCAGGTGTGCATCAAATTAGCAGACATCAACGGCCCAGCAAAAGATCGGGATCTTCATTTGAGATGGA
  CAGAAGGCATTGTGAATGAGTTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCC
  CTTCATGGATCGTTCTTCTCCTCAACTAGCAAAACTCCAAGAGTCATTTATCACCCACATTGTGGGCCCC
  CTGTGCAACTCCTATGATGCTGCTGGCTTGCTGCCTGGTCAGTGGATAGAAGCAGAAGAGGGTGACGATA
  CAGAAAGTGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGAAGAGTTAGATTCAGA
  TGATGAAGAAACAGAAGACAATCTAAATCCTAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGC
  CAACTAATGCACCACCTCACTGAAAACCACAAGATCTGGAAGGAAATCATAGAAGAGGAAGAAAAATGTA
  AAGCTGAGGGGAACAAACTGCAGGTGGATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTCATTGA
  AGAAGCAGATGAAGAGGAAGAACAAATGTTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209284 representing NM_017229
  Red=Cloning site Green=Tags(s)
  
  MRKDERERDTPAMRSPPPPPPPATATAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAAS
  LRAGARLSLAALAAFVLAALLGAGPERWAAAATGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGP
  DRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFA
  GLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTADEKVPVIRPRRRSS
  CVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDL
  LIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSSKPSFPT
  AQLRRSSGASGLLTSEHHSRWDRSGGKRPYQELSVSSHGCHLNGPFSSNLMTIPKQRSSSVSLTHHAGLR
  RAGALPSPSLLNSSSHVPVSAGCLTNRSPVGFLDTSDFLTKPSVTLHRSLGSVSSAADFHQYLRNSDSSL
  CSSCGHQILKYVSTCEPDGTDHHNEKSGEEDSTVFSKERLNIVETQEEETVKEDCRELFLEGDDHLMEEA
  QQPNIDQEVLLDPMLVEDYDSLIEKMSNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQ
  EFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLQQLHNNHETETKADSDARLSSGQIAYLS
  SKSCCIPDKSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASA
  WNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLV
  CQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGP
  LCNSYDAAGLLPGQWIEAEEGDDTESDDDDDDDDDDDDDDDEELDSDDEETEDNLNPKPQRRKGRRRIFC
  QLMHHLTENHKIWKEIIEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017229
• ORF Size: 3324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017229.1, NP_058925.1
• RefSeq Size: 4381
• RefSeq ORF: 3327
• Locus ID: 29516
• MW: 123.1 kDa
• Gene Summary: catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006]