Pde3b (NM_017229) Rat Untagged Clone

CAT#: RN209284

Pde3b (untagged ORF) - Rat phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10 ug)

Product Images

Circular map for RN209284

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pde3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_017229 ORF sequence, RN209284 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGAAGGACGAGCGCGAGCGGGACACGCCAGCCATGAGGTCCCCGCCGCCGCCGCCGCCGCCGGCC
  ACGGCCACGGCCGCCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTG
  CGGCAGGACCCTCCGCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCC
  TCGCTCCGCGCCGGGGCACGCCTCTCGCTCGCCGCTCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGC
  GCGGGGCCCGAGCGCTGGGCGGCCGCGGCGACCGGGCTTCGGACGCTGCTGAGCGCTTGCTCGCTCAGC
  CTCAGCCCGCTCTTCAGCATCGCCTGTGCCTTCTTCTTCCTCACCTGCTTCCTGACGCGCGCGCAGCGC
  GGCCCGGACCGCGGCGCCGGCTCCTGGTGGCTGCTGGCGCTGCCTGCCTGCTGCTACCTGGGCGACTTC
  GCGGCGTGGCAGTGGTGGTCGTGGCTGCGCGGGGAGCCGGCGGCGGCGGCGGCCGGCCGGCTCTGCCTG
  GTGCTGAGCTGCGTGGGGCTGCTGACGCTCGCGCCTCGCGTGAGGCTGCGGCACGGAGTCCTGGTGCTG
  CTCTTCGCCGGCCTGGTGTGGTGGGTGTCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCT
  CTGCTGTCGTGCCTGGTCGGGGGCGCGGGATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTC
  CGGGGAGCCTCACCTCCCCCGCGCTCGGCGAGTACCGCGGATGAAAAAGTGCCTGTGATCAGACCCCGG
  AGGAGGTCCAGCTGCGTGTCGCTGGGAGAAAGCGCGGCCGGTTACTATGGCAGTGGCAAGATGTTCAGG
  AGACCGTCGTTGCCTTGTATTTCTCGAGAACAGATGATTCTCTGGGACTGGGACTTGAAGCAGTGGTGT
  AAACCTCATTACCAGAATTCTGGAGGTGGAAATGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAAT
  ATGGTGTCAGACCTTCTGATTGACCCAAGCCTTCCCCCACAAGTCATTTCTTCTTTGCGGAGTATCAGT
  AGCTTGATGGGTGCTTTCTCAGGTTCCTGTAGGCCAAAGATTAATTCTTTCACACCATTTCCTGGATTT
  TATCCCTGTTCTGAAGTAGAAGATCCAGTAGAGAAAGGCGATCGAAAACTTCACAAGGGGTTGAGTAGC
  AAACCCAGTTTCCCGACTGCACAGCTGAGGAGGAGCTCAGGAGCGTCAGGATTGCTGACCAGTGAGCAC
  CACTCACGGTGGGATCGCAGCGGTGGCAAAAGGCCTTACCAAGAACTCAGTGTTTCAAGTCATGGATGC
  CACCTAAATGGGCCTTTTAGTTCAAATCTTATGACAATTCCAAAGCAGAGGTCATCATCTGTGTCGCTG
  ACGCACCATGCAGGTCTGAGAAGAGCTGGTGCTTTGCCCAGCCCCAGTCTTCTGAATTCTTCAAGCCAT
  GTACCCGTGTCTGCTGGCTGTCTAACTAATCGATCACCCGTAGGATTTCTTGATACCAGTGATTTTCTT
  ACTAAGCCAAGTGTTACTTTACATAGATCACTGGGCAGTGTATCCAGTGCAGCAGATTTCCATCAGTAC
  CTTAGGAACTCTGACAGCAGTCTGTGTAGCAGCTGTGGACACCAAATACTCAAGTATGTTTCGACATGT
  GAACCTGATGGTACAGACCACCACAATGAAAAATCAGGTGAAGAAGACAGCACTGTTTTCTCAAAAGAA
  CGATTGAACATTGTGGAAACTCAAGAAGAAGAGACAGTGAAGGAAGACTGCAGGGAATTATTTTTGGAA
  GGTGATGATCACCTAATGGAAGAGGCCCAGCAACCGAATATTGACCAGGAAGTGTTATTGGACCCGATG
  TTAGTGGAAGATTATGATTCATTAATAGAAAAGATGAGCAACTGGAACTTTCAGATTTTTGAGCTTGTA
  GAAAAAATGGGAGAAAAATCAGGAAGGATTCTCAGCCAGGTTATGTATACTTTATTTCAAGATACTGGT
  TTATTGGAAACATTTAAAATTCCCACTCAAGAATTTATGAATTATTTTCGTGCACTAGAAAATGGCTAC
  CGAGATATTCCATATCACAATCGTGTGCATGCCACAGATGTCCTACATGCTGTTTGGTATTTGACAACA
  CGACCAATTCCTGGCTTACAGCAGCTCCATAATAACCATGAAACAGAAACCAAAGCAGATTCAGATGCT
  AGACTTAGTTCTGGACAGATTGCTTACCTTTCTTCGAAGAGTTGCTGTATTCCAGATAAGAGTTATGGC
  TGCCTGTCTTCAAACATTCCTGCGTTAGAACTGATGGCTTTATATGTGGCAGCTGCCATGCACGATTAT
  GATCACCCAGGAAGAACAAATGCATTCCTAGTGGCTACAAATGCACCTCAGGCAGTTTTATACAATGAC
  AGATCTGTTCTAGAAAATCATCATGCCGCATCAGCGTGGAATCTGTATCTTTCTCGCCCAGAATACAAC
  TTCCTCCTTAACCTTGATCACATGGAATTCAAGCGTTTTCGATTTTTAGTCATAGAAGCAATCCTTGCT
  ACAGATCTCAAAAAACATTTTGAGTTCCTTGCTGAATTCAATGCCAAGGCCAATGATGTAAATAGTAAC
  GGTATAGAATGGAGCAGTGAAAACGATCGACTCTTAGTCTGCCAGGTGTGCATCAAATTAGCAGACATC
  AACGGCCCAGCAAAAGATCGGGATCTTCATTTGAGATGGACAGAAGGCATTGTGAATGAGTTTTATGAG
  CAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCCCTTCATGGATCGTTCTTCTCCTCAACTA
  GCAAAACTCCAAGAGTCATTTATCACCCACATTGTGGGCCCCCTGTGCAACTCCTATGATGCTGCTGGC
  TTGCTGCCTGGTCAGTGGATAGAAGCAGAAGAGGGTGACGATACAGAAAGTGATGATGATGATGATGAT
  GATGATGATGATGATGATGATGATGATGATGATGATGAAGAGTTAGATTCAGATGATGAAGAAACAGAA
  GACAATCTAAATCCTAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGCCAACTAATGCACCAC
  CTCACTGAAAACCACAAGATCTGGAAGGAAATCATAGAAGAGGAAGAAAAATGTAAAGCTGAGGGGAAC
  AAACTGCAGGTGGATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTCATTGAAGAAGCAGATGAA
  GAGGAAGAACAAATGTTTGAATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MRKDERERDTPAMRSPPPPPPPATATAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAA
  SLRAGARLSLAALAAFVLAALLGAGPERWAAAATGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQR
  GPDRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVL
  LFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTADEKVPVIRPR
  RRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARN
  MVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSS
  KPSFPTAQLRRSSGASGLLTSEHHSRWDRSGGKRPYQELSVSSHGCHLNGPFSSNLMTIPKQRSSSVSL
  THHAGLRRAGALPSPSLLNSSSHVPVSAGCLTNRSPVGFLDTSDFLTKPSVTLHRSLGSVSSAADFHQY
  LRNSDSSLCSSCGHQILKYVSTCEPDGTDHHNEKSGEEDSTVFSKERLNIVETQEEETVKEDCRELFLE
  GDDHLMEEAQQPNIDQEVLLDPMLVEDYDSLIEKMSNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTG
  LLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLQQLHNNHETETKADSDA
  RLSSGQIAYLSSKSCCIPDKSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYND
  RSVLENHHAASAWNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFEFLAEFNAKANDVNSN
  GIEWSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQL
  AKLQESFITHIVGPLCNSYDAAGLLPGQWIEAEEGDDTESDDDDDDDDDDDDDDDDDDEELDSDDEETE
  DNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADE
  EEEQMFE*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Fully Sequenced ORF

  >RN209284 representing NM_017229
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGGACGAGCGCGAGCGGGACACGCCAGCCATGAGGTCCCCGCCGCCGCCGCCGCCGCCGGCCA
  CGGCCACGGCCGCCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCG
  GCAGGACCCTCCGCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCG
  CTCCGCGCCGGGGCACGCCTCTCGCTCGCCGCTCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGG
  GGCCCGAGCGCTGGGCGGCCGCGGCGACCGGGCTTCGGACGCTGCTGAGCGCTTGCTCGCTCAGCCTCAG
  CCCGCTCTTCAGCATCGCCTGTGCCTTCTTCTTCCTCACCTGCTTCCTGACGCGCGCGCAGCGCGGCCCG
  GACCGCGGCGCCGGCTCCTGGTGGCTGCTGGCGCTGCCTGCCTGCTGCTACCTGGGCGACTTCGCGGCGT
  GGCAGTGGTGGTCGTGGCTGCGCGGGGAGCCGGCGGCGGCGGCGGCCGGCCGGCTCTGCCTGGTGCTGAG
  CTGCGTGGGGCTGCTGACGCTCGCGCCTCGCGTGAGGCTGCGGCACGGAGTCCTGGTGCTGCTCTTCGCC
  GGCCTGGTGTGGTGGGTGTCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGT
  GCCTGGTCGGGGGCGCGGGATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTC
  ACCTCCCCCGCGCTCGGCGAGTACCGCGGATGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGC
  TGCGTGTCGCTGGGAGAAAGCGCGGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGC
  CTTGTATTTCTCGAGAACAGATGATTCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCA
  GAATTCTGGAGGTGGAAATGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTT
  CTGATTGACCCAAGCCTTCCCCCACAAGTCATTTCTTCTTTGCGGAGTATCAGTAGCTTGATGGGTGCTT
  TCTCAGGTTCCTGTAGGCCAAAGATTAATTCTTTCACACCATTTCCTGGATTTTATCCCTGTTCTGAAGT
  AGAAGATCCAGTAGAGAAAGGCGATCGAAAACTTCACAAGGGGTTGAGTAGCAAACCCAGTTTCCCGACT
  GCACAGCTGAGGAGGAGCTCAGGAGCGTCAGGATTGCTGACCAGTGAGCACCACTCACGGTGGGATCGCA
  GCGGTGGCAAAAGGCCTTACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAG
  TTCAAATCTTATGACAATTCCAAAGCAGAGGTCATCATCTGTGTCGCTGACGCACCATGCAGGTCTGAGA
  AGAGCTGGTGCTTTGCCCAGCCCCAGTCTTCTGAATTCTTCAAGCCATGTACCCGTGTCTGCTGGCTGTC
  TAACTAATCGATCACCCGTAGGATTTCTTGATACCAGTGATTTTCTTACTAAGCCAAGTGTTACTTTACA
  TAGATCACTGGGCAGTGTATCCAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAGTCTG
  TGTAGCAGCTGTGGACACCAAATACTCAAGTATGTTTCGACATGTGAACCTGATGGTACAGACCACCACA
  ATGAAAAATCAGGTGAAGAAGACAGCACTGTTTTCTCAAAAGAACGATTGAACATTGTGGAAACTCAAGA
  AGAAGAGACAGTGAAGGAAGACTGCAGGGAATTATTTTTGGAAGGTGATGATCACCTAATGGAAGAGGCC
  CAGCAACCGAATATTGACCAGGAAGTGTTATTGGACCCGATGTTAGTGGAAGATTATGATTCATTAATAG
  AAAAGATGAGCAACTGGAACTTTCAGATTTTTGAGCTTGTAGAAAAAATGGGAGAAAAATCAGGAAGGAT
  TCTCAGTCAGGTTATGTATACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAA
  GAATTTATGAATTATTTTCGTGCACTAGAAAATGGCTACCGAGATATTCCATATCACAATCGTGTGCATG
  CCACAGATGTCCTACATGCTGTTTGGTATTTGACAACACGACCAATTCCTGGCTTACAGCAGCTCCATAA
  TAACCATGAAACAGAAACCAAAGCAGATTCAGATGCTAGACTTAGTTCTGGACAGATTGCTTACCTTTCT
  TCGAAGAGTTGCTGTATTCCAGATAAGAGTTATGGCTGCCTGTCTTCAAACATTCCTGCGTTAGAACTGA
  TGGCTTTATATGTGGCAGCTGCCATGCACGATTATGATCACCCAGGAAGAACAAATGCATTCCTAGTGGC
  TACAAATGCACCTCAGGCAGTTTTATACAATGACAGATCTGTTCTAGAAAATCATCATGCCGCATCAGCG
  TGGAATCTGTATCTTTCTCGCCCAGAATACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGTT
  TTCGATTTTTAGTCATAGAAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCTGAATT
  CAATGCCAAGGCCAATGATGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGACTCTTAGTC
  TGCCAGGTGTGCATCAAATTAGCAGACATCAACGGCCCAGCAAAAGATCGGGATCTTCATTTGAGATGGA
  CAGAAGGCATTGTGAATGAGTTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCC
  CTTCATGGATCGTTCTTCTCCTCAACTAGCAAAACTCCAAGAGTCATTTATCACCCACATTGTGGGCCCC
  CTGTGCAACTCCTATGATGCTGCTGGCTTGCTGCCTGGTCAGTGGATAGAAGCAGAAGAGGGTGACGATA
  CAGAAAGTGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGAAGAGTTAGATTCAGA
  TGATGAAGAAACAGAAGACAATCTAAATCCTAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGC
  CAACTAATGCACCACCTCACTGAAAACCACAAGATCTGGAAGGAAATCATAGAAGAGGAAGAAAAATGTA
  AAGCTGAGGGGAACAAACTGCAGGTGGATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTCATTGA
  AGAAGCAGATGAAGAGGAAGAACAAATGTTTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_017229
• Insert Size: 3336 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017229.2
• RefSeq Size: 5368 bp
• RefSeq ORF: 3327 bp
• Locus ID: 29516
• UniProt ID: Q63085
• Cytogenetics: 1q34
• MW: 123.5 kDa
• Gene Summary: catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006]