Cyp2u1 (NM_001024779) Rat Tagged ORF Clone

CAT#: RR209549

Cyp2u1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 2, subfamily u, polypeptide 1 (Cyp2u1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp2u1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209549 representing NM_001024779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGATCGGGGGCCTGCGTCCCGCGGCCGGGGAGCAGCCGGGAGTTGGGCCGCACCTACAGGCGG
  TGGGAGGCGCGCTGCTGCTTTGCGGGCTGGCGGTCCTGCTGGACTGGGTTTGGCTGCAGCGGCAGCGCGC
  CGGCGGCATCCCTCCGGGGCCCAAGCCTCGGCCACTCGTGGGTAACTTCGGGTACTTGCTGCTGCCGCGC
  TTCCTACGGCTGCACTTCTGGCTGGGCTCGGGCAGCCAGACCGACACAGTAGGCCGGCATGTGTACCTGG
  CCAGGCTGGCCCGTGTCTATGGCAACATCTTCAGCTTCTTCATCGGCCACCGTCTGGTGGTGGTCCTCAG
  CGACTTCCAGAGCGTGCGCGAGGCGCTTGTGCAGCAGGCCGAAGTGTTCAGTGACCGCCCGCGGATGCCG
  CTCATCTCCATCTTGACCAAGGAGAAGGGAATCGTGTTCGCACACTATGGTCCGATCTGGAAGCAGCAGA
  GAAGATTCTCCCACTCAACCCTTCGTCACTTCGGCCTGGGCAAGCTTAGCCTGGAGCCCAGGATCATCGA
  GGAGTTCGCGTACGTGAAAGCAGAGATGCAGAAGCACGGGGAGGCCCCCTTCAGCCCCTTCCCAGTCATC
  AGCAATGCCGTCTCCAACATCATCTGCTCCCTGTGCTTCGGCCAGCGCTTCGACTACACCAACAAGGAGT
  TTAAAAAGGTGCTGGACTTCATGTCTCGAGGGCTAGAAATCTGCTTGCACAGCCAGCTCTTCCTGATCAA
  CCTATGTCCCTGGTTTTACTACCTTCCCTTTGGGCCGTTTAAGGAACTGAGGCAAATCGAAAGGGACATA
  ACCTGCTTCCTTAAAAACATCATCAAAGAGCACCAAGAGTCTCTGGATGCCAACAACCCCCAGGACTTCA
  TAGACATGTACCTTCTGCACACGCAGGAGGAGAAGGACAAGTGCAAGGGCACCAACTTCGATGAGGACTA
  CCTGTTCTACATCATCGGGGACCTCTTCATCGCGGGCACGGACACCACAACCAACTCTTTGCTCTGGTGC
  CTGCTGTACATGTCACTGAACCCCGGCGTGCAAAAAAAGGTTCATGAAGAAATTGAAAGGGTCATTGGCC
  GTGACCGTGCACCTTCCCTCACAGACAAGGCCCAGATGCCATACACAGAAGCCACCATCATGGAGGTACA
  GCGGTTATCCATGGTGGTACCACTTGCCATTCCTCACATGACCTCGGAGAAAACAGTCCTCCAAGGGTAC
  AGTATTCCTAAAGGCACAGTGGTCCTACCCAATTTGTGGTCCATACACAGAGACCCAGTCATTTGGGAGA
  AGCCAGACGACTTCTGTCCTCATCGATTTCTGGATGACCAGGGACAACTTTTGAAAAGAGAAACGTTTAT
  CCCTTTTGGGATAGGGAAGCGGGTGTGTATGGGAGAGCAGCTGGCCAAGATGGAGCTATTCCTGATGTTT
  GTGAGCCTGATGCAGAGTTTCACCTTTGCTTTACCTGAGGGCTCCGAGAAGCCCATCATGACCGGCAGAT
  TTGGTCTAACATTGGCCCCACATCCCTTTAATGTAACCGTCTCAAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209549 representing NM_001024779
  Red=Cloning site Green=Tags(s)
  
  MSSIGGLRPAAGEQPGVGPHLQAVGGALLLCGLAVLLDWVWLQRQRAGGIPPGPKPRPLVGNFGYLLLPR
  FLRLHFWLGSGSQTDTVGRHVYLARLARVYGNIFSFFIGHRLVVVLSDFQSVREALVQQAEVFSDRPRMP
  LISILTKEKGIVFAHYGPIWKQQRRFSHSTLRHFGLGKLSLEPRIIEEFAYVKAEMQKHGEAPFSPFPVI
  SNAVSNIICSLCFGQRFDYTNKEFKKVLDFMSRGLEICLHSQLFLINLCPWFYYLPFGPFKELRQIERDI
  TCFLKNIIKEHQESLDANNPQDFIDMYLLHTQEEKDKCKGTNFDEDYLFYIIGDLFIAGTDTTTNSLLWC
  LLYMSLNPGVQKKVHEEIERVIGRDRAPSLTDKAQMPYTEATIMEVQRLSMVVPLAIPHMTSEKTVLQGY
  SIPKGTVVLPNLWSIHRDPVIWEKPDDFCPHRFLDDQGQLLKRETFIPFGIGKRVCMGEQLAKMELFLMF
  VSLMQSFTFALPEGSEKPIMTGRFGLTLAPHPFNVTVSKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024779
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024779.1, NP_001019950.1
• RefSeq Size: 2154 bp
• RefSeq ORF: 1593 bp
• Locus ID: 310848
• Cytogenetics: 2q43
• MW: 60.4 kDa