Kcnj1 (NM_017023) Rat Tagged ORF Clone

CAT#: RR209907

Kcnj1 (Myc-DDK-tagged ORF) - Rat potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj1
• Synonyms: KAB-1; Kcnj; Kcnj1_v1; Kcnj1_v3; kir1.1; ROMK1; ROMK2; ROMK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209907 representing NM_017023
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCTTCGGAACGGAGTGTGTTCAGAGTGCTGATCAGGGCACTGACAGAAAGGATGTTCAAACACC
  TCCGAAGATGGTTTGTCACTCACATATTTGGGCGTTCCCGGCAACGGGCAAGGCTGGTCTCTAAAGAAGG
  AAGATGTAACATCGAGTTTGGCAATGTGGATGCACAGTCAAGGTTTATATTCTTTGTGGACATCTGGACA
  ACTGTGCTGGACCTGAAATGGAGGTACAAAATGACCGTGTTCATCACAGCCTTCTTGGGGAGTTGGTTCC
  TCTTTGGTCTCCTGTGGTATGTCGTAGCGTATGTTCATAAGGACCTCCCAGAGTTCTACCCGCCTGACAA
  CCGCACTCCTTGTGTGGAGAACATTAATGGCATGACTTCAGCCTTTCTGTTTTCTCTAGAGACTCAAGTG
  ACCATAGGTTACGGATTCAGGTTTGTGACAGAACAGTGCGCCACTGCCATTTTCCTGCTTATCTTCCAAT
  CTATTCTTGGAGTGATCATCAATTCCTTCATGTGTGGTGCCATTTTAGCCAAGATCTCTAGACCTAAAAA
  ACGTGCTAAAACCATTACGTTCAGCAAGAATGCGGTGATCAGCAAGCGTGGCGGGAAGCTCTGCCTCCTC
  ATCCGAGTGGCCAATCTTAGGAAGAGCCTTCTGATTGGCAGCCACATATATGGCAAGCTTCTAAAGACAA
  CCATCACTCCTGAAGGCGAGACCATCATTTTGGATCAGACCAACATCAACTTTGTCGTCGACGCTGGCAA
  TGAAAATTTGTTCTTCATATCCCCACTGACGATCTACCACATTATTGACCACAACAGCCCTTTCTTCCAC
  ATGGCAGCAGAAACTCTTTCCCAACAGGACTTTGAGCTGGTGGTCTTTTTAGATGGCACAGTGGAATCCA
  CCAGTGCAACCTGCCAGGTCCGCACGTCATACGTCCCAGAGGAGGTACTTTGGGGCTACCGTTTCGTTCC
  TATTGTGTCCAAGACCAAGGAAGGGAAATACCGAGTGGATTTTCATAACTTCGGTAAGACAGTGGAAGTG
  GAGACCCCTCACTGTGCCATGTGCCTCTATAATGAGAAAGATGCCAGGGCCAGGATGAAGAGAGGCTATG
  ACAACCCTAACTTTGTCTTGTCAGAAGTTGATGAAACGGACGACACCCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209907 representing NM_017023
  Red=Cloning site Green=Tags(s)
  
  MGASERSVFRVLIRALTERMFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWT
  TVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQV
  TIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLL
  IRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFH
  MAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEV
  ETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017023
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017023.1, NM_017023.2, NP_058719.2
• RefSeq Size: 2999
• RefSeq ORF: 1176
• Locus ID: 24521
• MW: 44.9 kDa
• Gene Summary: potassium channel involved in K+ secretion in the renal distal nephron [RGD, Feb 2006]